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活力测定

活力测定的相关文献在1983年到2022年内共计200篇,主要集中在生物化学、化学工业、药学 等领域,其中期刊论文146篇、会议论文27篇、专利文献79534篇;相关期刊123种,包括生物化学与生物物理进展、中国麻风皮肤病杂志、中国药学杂志等; 相关会议23种,包括2015年中国马铃薯大会、中国作物学会作物种子专业委员会2015年学术年会、2015年陕西省食品科学技术学会学术年会等;活力测定的相关文献由602位作者贡献,包括彭必雨、王进、颜霞等。

活力测定—发文量

期刊论文>

论文:146 占比:0.18%

会议论文>

论文:27 占比:0.03%

专利文献>

论文:79534 占比:99.78%

总计:79707篇

活力测定—发文趋势图

活力测定

-研究学者

  • 彭必雨
  • 王进
  • 颜霞
  • 万树栋
  • 张春晓
  • 李津婴
  • 罗凤香
  • 高蒙初
  • 丁家宜
  • 严谈松
  • 期刊论文
  • 会议论文
  • 专利文献

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    • 周希梅; 赵华; 黄萌萌; 李竞前; 闫奎友
    • 摘要: 本文重点介绍了凝乳酶的种类和凝乳机理,以及自制羔羊凝乳酶及其活力测定的方法,对引导我国家庭牧场和小型羊乳企业突破凝乳酶自制核心技术,创新开展羊奶酪生产研发,填补国产羊奶酪市场空白与丰富羊乳制品种类,具有重要的指导意义。
    • 黄青; 鲁素雅; 汪岱迪; 金卫红; 刘洋; 陆益红; 史清水
    • 摘要: 目的 优化现行标准中用于测定转移因子制剂活力的脱E受体法,并建立可用于替代的新方法,用上述方法对来自3个生产企业的57批转移因子制剂进行测定.方法 采用改进的细胞涂片-染色法制备样品的细胞涂片;采用新鲜猪胸腺、兔胸腺分别与绵羊红细胞进行交叉试验;建立微量板-白细胞黏附抑制替代方法,对方法的线性、重复性、专属性等进行考察.结果 改进的涂片-染色法,镜检效果清晰可靠,便于读数和保存.猪胸腺淋巴细胞与兔胸腺淋巴细胞均能与绵羊红细胞形成E玫瑰花结,两种方法试验结果无显著差异.白细胞黏附抑制率结果与玫瑰花结活力测定结果无显著性差异,可用于替代.结论 以改进的染色涂片方法替代原有的细胞计数板法,能更清晰的反应样品的活力值,且可长期保存,适合对大批量样品的随时读片和复核.微量板-白细胞黏附试验替代方法,降低了人为主观因素,较原方法更加准确、快速、重现性好、专属性强,且适合大批量样本的高通量检测.
    • 陈忠文; 周黎; 王德英; 黄超; 文西强; 王宏飞; 潘晶晶
    • 摘要: It is important link to understanding the pollen viability of jicama for crossbreeding.The results indicated that there were significantly differences for the pollen viability of jicama measured by methods of culture in vitro,TTC and I2-KI.For the same viability pollen,the pollen viability was within 36% by culture in vitro,while the pollen viability was more than 92% by I2-KI.There were also differences in the pollen viability of different varieties by the same test method.The pollen germination result was the best when the pollen cultured for 5 ~ 6 h in vitro with 20% of sucrose,10 mg/L of boric acid,pH 7.22.The staining time of I2-KI was the fastest and dyeing deeply,its appropriate concentration was 0.5%.%了解豆薯花粉活力是开展杂交育种的重要环节.通过对豆薯不同品种花粉活力测定表明:离体培养、TTC法和I2-KI法测定豆薯花粉活力结果差异较大,以花粉离体培养测定花粉活力在36%以内,I2-KI法测定染色效果最为明显达92%以上.不同品种采用同一方法测定的花粉活力亦有差异.花粉离体培养萌发在蔗糖浓度20%、硼酸浓度10 mg/L,pH值7.22条件下5~6h观察结果最佳.I2-KI染色时间快、染色深,浓度以0.5%为宜.
    • 傅丹桂; 孙雁; 黄正仙; 姚宗泽; 何丽萍
    • 摘要: [目的]为筛选出一种或多种与田间出苗试验密切相关的室内水稻种子活力测定方法.[方法]选取不同类型的8个水稻品种为材料,开展标准发芽、幼苗生长、胚根突出、加速老化、冷处理发芽以及田间出苗试验.[结果]冷处理试验、25°C胚根突出试验第96小时和30°C第66小时的发芽率均与田间出苗率呈极显著正相关,相关系数分别为0.942和0.876;而幼苗生长试验25°C第10天的幼苗芽长(r=?0.848)和30°C第7天的幼苗芽长(r=?0.902)与田间出苗率呈极显著负相关关系.同时冷处理试验与胚根突出25°C、96 h之间呈极显著相关(r=0.913)、与胚根突出试验30°C、66 h(r=0.797)以及30°C第7天的幼苗芽长(r=?0.761)为显著相关.此外,30°C第7天的幼苗芽长与胚根突出试验25°C、96 h的计数(r=?0.750)显著相关;胚根突出试验中的25°C第96小时计数和30°C第66小时计数为极显著相关(r=0.872).[结论]冷处理发芽试验、胚根突出试验(25°C第96小时和30°C第66小时计数)可推荐作为水稻种子活力测定方法.%[Purpose]The aim of this study was to find one or more inexpensive, uncomplicated, rapid, objective, reproducible, and related to field emergence for rice seeds vigor testing methods.[Method]In this study, 8 different type rice varieties as experiment materials, the following tests were used which containing: standard germination test, seedling growth test (the root length and shoot length of 7, 10, 14 days were measured at 25 °C and 30 °C, respectively), radicle emergence test (25 °C and 30 °C), accelerated aging test, cold treatment test and field emergence test. [Results]The results of correlation analysis between tests showed that the first cold treatment test, radicle emergence test of 25 °C at 96 h (r=0.942) and 30 °C at 66 h (r=0.876), and 25 °C seedling growth test on the 10 d of shoot length (r=?0.848) and on the 7 d of the shoot length (r=?0.902) were extremely significantly correlated with field emergence. Secondly, the cold treatment test and radicle emergence test of 25 °C at 96 h (r=0.913) was extremely significant correlation, and radicle emergence test of 30 °C at 66 h (r=0.876) was significantly correlated. The radicle emergence test between 25 °C by 96 h count and30 °C by 66 h count was extremely significant correlation (r=0.872). [Conclusion]In view of the above results, cold treatment test, the radicle emergence test (25 °C by 96 h and 30 °C by 66 h count) should be recommendation for a vigor testing methods in rice seeds.
    • 张延波; 王佩玲; 赵会利; 张平
    • 摘要: 以番茄小果型材料10876、中果型材料10869、大果型材料11559为试验材料,在室温(25°C)、4°C、-20°C条件下,采用红墨水染色法研究了不同温度条件下不同储藏时间对番茄花粉活力的影响.结果表明,中果型材料花粉活力下降最快,以花粉活力50%为下限,小果型和大果型材料在室温下可以保存8 h,中果型可以保存4 h;4°C条件下,小果型和大果型材料可以保存10 d,中果型可以保存8 d;在-20°C环境中,小果型和大果型材料可以保存28 d,中果型可以保存20 d.
    • 王乐; 刘松; 尹艳丽; 黄巍; 吕扬勇; 胡元森; 惠明; 王金水
    • 摘要: Objective To separate out and identify a high yield strain for acid lipase production. Methods A strain of acid lipase was obtained by preliminary screening with plates containing the culture medium of the bromocresol purple indicator, and secondary screening by the transparent circle method with glycerin triglyceride butyrate. The enzyme activity of the screened strain was determined by the methods of plate transparent ring, olive oil emulsion titration and the method of colorimetry. Results A strain of high yield acid lipase was isolated and named WY19, and its crude enzyme activity reached 11000 U/L. Through the morphological identification of the strains WY19, identification, physiological and biochemical experiments and molecular biology combined with the phylogenetic tree analysis, the high-producing strain WY19 was determined as Klebsiella. Conclusion The lipase of strain WY19 is an acid lipase, and it has good application prospects in the fields of food, medicine, oil processing.%目的 分离筛选出一株酸性脂肪酶高产菌株并对其进行鉴定.方法 通过溴甲酚紫酸碱指示剂进行平板初筛,甘油三丁酸酯透明圈法以及摇瓶培养复筛,筛选获得了一株酸性脂肪酶产生菌株;利用平板透明圈法、橄榄油乳化液滴定法和对硝基苯酚比色法3种方法对所筛菌株进行酶活力测定.结果 分离获得一株具有高产酸性脂肪酶活力的菌株,将其命名为菌株WY19.经过检测,其粗酶活力达到11000 U/L.通过对菌株WY19进行形态学鉴定、生理生化实验以及分子生物学鉴定,结合系统发育树分析,确定本研究获得的酸性脂肪酶产生菌为克雷伯氏菌.结论 菌株WY19所产脂肪酶为酸性脂肪酶,在食品、医药、油脂加工等领域方面具有较好的应用前景.
    • 杨媛; 张剑
    • 摘要: The activity of lipase was determined by alkali titration method and the conditions for the determination of lipase activity were studied. Results showed that the lipase activity is stable when the volume fraction of olive oil is 25% and the temperature is 40 °C,pH =7.5. Under such conditions,the effects of adding anionic and nonionic surfactants were investigated. Results showed that effect of nonionic surfactants on the activity of lipase is less than that of anionic surfactants. Finally,the analysis method was applied to determine the activity of lipase in the commercial detergent products. Results showed that the method can be used for the determination of lipase activity in both liquid detergent and laundry powder. However,the temperature and pH value of the detergent solution should be controlled strictly during the conduction of the determination.%采用碱滴定法测定脂肪酶活力,结果显示,在橄榄油体积分数为25%,温度为40°C,pH=7.5时酶活力较为稳定。在较佳条件下,考察了添加阴离子、非离子表面活性剂对测定脂肪酶活力的影响,实验结果表明,非离子表面活性剂对测定脂肪酶活力的影响较小。将该分析方法应用于市售洗涤产品中脂肪酶活力的测定,研究结果显示,方法可用于液体洗涤剂和洗衣粉中脂肪酶活力的测定,但需严格控制洗涤剂或洗衣粉溶液的温度及pH。
    • 余燕; 梁蔚阳; 邓锋; 蒋玉辉
    • 摘要: 目的 评价脑蛋白水解物对H2O2诱导的PC12细胞损伤的修复效果,建立一种脑蛋白水解物注射液的生物活性检测方法.方法 以脑蛋白水解物的进口代表药物注射用脑蛋白水解物为例,采用PC12细胞培养,分析不同细胞接种浓度、不同浓度的H2O2损伤细胞、不同浓度脑蛋白水解物对抗干预的影响;采用MTT比色法对样品组、对照组和损伤组的细胞进行染色后,用酶标仪测定OD值,计算修复率,以评价脑蛋白水解物对模型细胞PC12损伤的保护作用;同时将该损伤修复评价方法用于国产脑蛋白水解物的生物活性检测.结果在 8×104~12×104·mL-1细胞接种量、0.5 mmol·L-1H2O2损伤浓度下、60μg·L-1药物浓度(以含氮量计)下可成功的建立H2O2诱导的PC12细胞损伤模型.结论 采用该方法对8批国产脑蛋白水解物注射液样品及注射用脑蛋白水解物进行对比检测,发现对H2O2损伤的细胞均具有良好修复作用.
    • 赵震锡; 苏斯思
    • 摘要: 以塔里木河下游采集的成熟胡杨种子为材料,采用室内实验法分别对不同贮藏条件下胡杨种子活力和不同水分胁迫下的胡杨种子发芽率进行了研究.结果表明,胡杨种子不耐贮藏,4°C密封贮藏能够有效延长种子寿命;不同浓度的PEG胁迫处理对种子的萌发均有一定的抑制作用,种子的发芽率、发芽势随胁迫强度的增加呈现明显下降趋势;26%的PEG胁迫处理时,胡杨种子的发芽率和发芽势相对保持在较高水平,可以将其作为评价种子活力状况的关键浓度.
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