摘要：
对番茄红素纳米结构脂质体(lyco-NLC)的制备工艺进行了研究,以产品的平均粒径、多分散性系数(PDI)和包封率为指标,通过单因素实验和正交实验研究了物料因素和操作因素对lyco-NLC的影响,确定最佳制备工艺.并对lyco-NLC进行了形貌观察和贮藏稳定性研究.结果表明,lyco-NLC的最佳制备工艺条件为:总脂质质量浓度7.5 g/100 mL,固液脂质量比3.5∶1,表面活性剂吐温80质量浓度2 g/100 mL,均质压力50 MPa,均质次数3次.在最佳制备工艺条件下,lyco-NLC平均粒径为230 nm,PDI为0.23,包封率达到91.95％,产品中番茄红素载量高达4％,具有很好的水分散性.透射电子显微镜结果表明lyco-NLC粒子呈规则的球形,分散均匀.30 d的贮藏实验表明lyco-NLC具有良好的物理稳定性(平均粒径＜270 nm,PDI＜0.3).%The preparation process of lycopene-loaded nanostructured lipid (lyco-NLC) was studied.With product average particle size,polydispersity index(PDI) and encapsulation efficiency as indexes,the influences of material and operation factors on lyco-NLC were studied using single factor experiment and orthogonal experiment to obtain the optimal preparation process.The morphology of lyco-NLC was observed,and its storage stability was studied.The results showed that the optimal preparation process conditions were obtained as follows:total lipid mass concentration 7.5 g/100 mL,mass ratio of solid lipid to liquid lipid 3.5∶ 1,surfactant Tween 80 mass concentration 2 g/100 mL,homogenization pressure 50 MPa,homogenization times 3.Under these conditions,the average particle size of lyco-NLC was 230 nm with PDI 0.23,the encapsulation efficiency was 91.95％ and the lycopene loading amount in the product was up to 4％.The product possessed good water dispersibility.Particles of the lyco-NLC were found to be spherical and uniform in shape under transmission electron microscopy (TEM).The 30 d storage experiment results showed that lyco-NLC had good physical stability (average particle size ＜270 nm,PDI ＜ 0.3).
