模型,生物学

摘要： 非酒精性脂肪性肝病(NAFLD)已成为我国第一大慢性肝病,以及欧美发达国家肝移植的主要病因.在NAFLD的各种表型中,非酒精性脂肪性肝炎(NASH)极有可能发展为包括肝硬化、肝癌在内的终末期肝病,导致肝脏相关的死亡率增加.但目前尚无针对NASH的治疗药物,许多新药正在开展临床试验中.NASH新药的研发,需要依据抗代谢、抗炎以及抗纤维化作用方式来选择合适的目标人群和治疗终点.总结和论述了目前NASH新药研发中的一些关键因素.

摘要： 乳腺癌已成为在全球范围内女性中发病率最高的恶性肿瘤,且其发病率仍以每年3％～4％的速度不断增长,严重威胁了女性的生活质量和生命健康.在乳腺癌的基础研究中,建立理想的乳腺癌模型至关重要.本文在分析了乳腺癌病因与分类、生物标志物和治疗靶点的基础上,着重阐述了在乳腺癌研究中常用到的2类细胞模型(二维细胞培养和三维细胞培养)及5类动物模型(自发、诱导、移植、基因工程和远处转移),并结合具体实验流程,详细地分析了各类模型适用的研究领域,帮助研究者了解其最新的研究进展,以期更好地探究乳腺癌的病因、机制、治疗和预防.最后,本文系统地比对了各种细胞模型与动物模型的优势与不足.

摘要： 大数据对肿瘤临床研究有非常重要的意义,骨肿瘤病理亚型众多,且发病率均不高,因此导致单一医疗机构的病例数据量比较少,目前国内一些大型的骨肿瘤诊治中心也建立了一定规模的数据库,如积水潭医院的骨肿瘤数据库[1],然而全国范围的骨肿瘤登记系统及大数据库仍然是空缺。本期刊出的柳昌全等[2]回顾性分析 1995 年至 2014 年,来自 SEER 数据库 1910 例四肢骨肉瘤患者,通过单因素 Log-rank 分析和多因素 COX 分析。

摘要： Objective To investigate the protective effect of berberine (BBR) on neuronal damage induced by oxygen-glucose deprivation (ODD) in HT22 mouse hippocampal neuronal cell,and the role of superoxide dismutase 2 (SOD2) in it.Methods HT22 cells were exposed to OGD for 4 h and then reoxygenated for 24 h to simulate ischemia-reperfusion injury.The HT22 cells were divided into control group,OGD group,BBR + OGD group,SOD2-siRNA + BBR + OGD group,and scrambled (SC)-siRNA + BBR + OGD group.Cell viability was measured by thiazole blue method.Cell morphology was observed by phase contrast microscopy.Medium lactate dehydrogenase (LDH) level,intracellular glutathione (GSH),and catalase (CAT) content were detected by colorimetric assay.The cell apoptosis rate was detected by flow cytometry.The expression level of cleaved caspase-3 was detected by Western blot analysis.Results Compared with the control group,OGD significantly decreased cell viability,intracellular GSH,and CAT level (all P ＜0.05),increased cell LDH release,apoptosis rate,and cleaved caspase-3 protein expression level (all P＜0.05).At the same time,cell morphology destruction was observed.BBR significantly reduced the above damage of HT22 cells induced by OGD (all P ＜0.05),while SOD2-siRNA significantly reversed the protective effect of BBR on HT22 cells (all P ＜0.05).Conclusions BBR significantly alleviated neuronal damage induced by recovery of oxygen-glucose after OGD.SOD2 might mediate its protective effect.%目的 探讨小蘖碱(berberine,BBR)对氧-葡萄糖剥夺(oxygen glucose deprivation,OGD)后复氧复糖所致HT22小鼠神经元损伤的保护作用,以及超氧化物歧化酶2(superoxide dismutase 2,SOD2)在其中所起的作用.方法 将HT22细胞暴露于OGD 4 h,然后复氧复糖24 h,模拟缺血再灌注损伤.将HT22细胞分为对照组、OGD组、BBR+ OGD组、SOD2-siRNA+ BBR+ OGD组和乱序(scrambled,SC)-siRNA+ BBR+ OGD组,采用噻唑蓝法检测细胞活力,相差显微镜观察细胞形态,酶标仪比色法检测培养基乳酸脱氢酶(lactic dehydrogenase,LDH)水平以及细胞内还原型谷胱甘肽(glutathione,GSH)和过氧化氢酶(catalase,CAT)含量,流式细胞术检测细胞凋亡率,蛋白质印迹分析检测凋亡相关蛋白cleaved caspase-3表达水平.结果 与对照组相比,OGD可显著降低细胞活力以及细胞内GSH和CAT水平(P均＜0.05),增加细胞LDH释放量、凋亡率以及cleaved caspase-3蛋白表达水平(P均＜0.05),同时观察到细胞形态破坏.BBR可显著减轻OGD所致HT22细胞损伤(P均＜0.05),而SOD2-siRNA可显著逆转BBR对HT22细胞的保护作用(P均＜0.05).结论 BBR可显著减轻OGD后复氧复糖所致神经元损伤,SOD2可能介导了其保护作用.

摘要： Objective:To observe inhibitory effect of zoledronic acid on polyethylene particle-induced periprosthetic osteolysis model.Methods:Thirty male adult specific-pathogen-free SD rats (weighted 250 to 300 g) were randomly divided into sham group,model group,and zoledronic acid group,10 in each group.Modeling were building by titanium screws and polyethylene particles implanted into right femur of rats,sham group and model group were performed hypodermic injection by 0.9％ saline with 2 ml/kg,zoledronic acid with 0.1 ml/mg were injected into zoledronic acid group,once a week for 8 weeks.After 8 weeks,right femur specimens were drawn and used to san microstructure of femoral cancellous bone in rats model was examined by Micro-CT,and the images were treated with three-dimension reconstruction and analysis software BMD,BV/TV,Tb.N,Tb.Th,SMI,BS/BV,Tb.Sp and Tb.Pf and other parameters include.Results:According to Micro-CT three-dimensional imaging,BMD in model group was significantly decreased than sham group,bone microstructure damage was serious,bone trabecula changed thinning continuity;while bone microstructure was obviously improved compared with model group and zoledronic acid group.After analyzing Micro-CT parameters of femur microstructure,BMD in model group (0.081±0.020) was significantly decreased than control group (0.160±0.018) and zoledronic acid group (0.125±0.012);BV/TV in model group (10.563±1.070) was obviously lower than control group(27.935±1.834) and zoledronic acid group(14.559±1.258);Tb.N in model group (1.005±0.165) was lower than control group (2.058±0.108) and zoledronic acid group (1.515±0.126);while Tb.Th in model group (0.075±0.016) was decreased than control group (0.158±0.016) and zoledronic acid group (0.124±0.011).Meanwhile,SM I in model group (1.817 ±0.127) was significantly higher than control group (1.104 ±0.120) and zoledronic acid group (1.547±0.122);BS/BV in model group (35.784±1.650) was higher than control group (21.506±2.771) and zoledronic acid group (30.399±2.730);Tb.Sp in model group (0.735±0.107) was higher than control group (0.423±0.057) and zoledronic acid group (0.577±0.082),TB.Pf in model group (9.088±1.283) was higher than control group (2.447±0.703) and zoledronic acid group (5.862±1.042).Conclusion:Zoledronic acid could change bone microstructure of rats to inhibit polyethylene particle-induced bone solution,which provides a scientific basis for prevent bone solution by zoledronic acid as a therapeutic intervention.%目的:研究唑来膦酸对磨损颗粒诱导假体周围骨溶解大鼠模型的骨微结构的影响.方法:选取30只成年雄性SPF级SD大鼠(重250～300 g),随机分成假手术组、模型组和唑来膦酸组,每组10只.通过往大鼠右侧股骨置入钛钉和聚乙烯颗粒进行造模,术后3d假手术组、模型组皮下注射0.9％生理盐水2ml/kg,唑来膦酸组皮下注射唑来磷酸0.1 mg/kg,每周1次,共8周.8周后取各组大鼠右侧股骨标本采用Micro-CT对大鼠股骨松质骨微结构进行扫描,应用三维重建处理和分析软件进行处理得到图像及BMD、BV/TV、Tb.N、Tb.Th、SMI、BS/BV、Tb.Sp、Tb.Pf等参数进行分析.结果:Micro-CT三维成像显示,模型组大鼠股骨骨密度较假手术组明显下降,骨微结构破坏严重,骨小梁稀疏变细,连续性降低;唑来膦酸组与模型组比较,骨微结构又出现明显改善.对大鼠股骨微结构的Micro-CT参数进行分析,模型组大鼠股骨BMD (0.081 ±0.020)明显低于假手术组(0.160±0.018)及唑来膦酸组(0.125±0.012),差异有统计学意义;模型组大鼠股骨BV/TV(10.563±1.070)低于假手术组(27.935±1.834)及唑来膦酸组(14.559±1.258),差异有统计学意义;模型组大鼠股骨Tb.N (1.005±0.165)低于假手术组(2.058±0.108)及唑来膦酸组(1.515±0.126),差异均有统计学意义;模型组大鼠股骨Tb.Th (0.075±0.016)明显低于假手术组(0.158±0.016)及唑来膦酸组(0.124±0.011),差异均有统计学意义.同时,模型组大鼠股骨SMI(1.817±0.127)明显高于假手术组(1.104±0.120)及唑来膦酸组(1.547±0.122),差异均有统计学意义;模型组大鼠股骨BS/BV(35.784±1.650)明显高于假手术组(21.506±2.771)及唑来膦酸组(30.399±2.730);模型组大鼠股骨Tb.Sp(0.735±0.107)高于假手术组(0.423±0.057)及唑来膦酸组(0.577±0.082),差异均有统计学意义;模型组大鼠股骨Tb.Pf(9.088±1.283)明显高于假手术组(2.447±0.703)及唑来膦酸组(5.862±1.042),差异有统计学意义.结论:唑来膦酸可以通过改变大鼠骨微结构达到抑制磨损颗粒诱发的骨溶解的作用,为使用唑类酸作为一种治疗性干预手段来防止假骨溶解提供了科学依据.

摘要： Objective To evaluate the effects of individual extralevator abdominalperineal excision (ELAPE) for rectal neoplasms below levator hiatus on pelvic floor by finite element analysis. Methods MIMICS 10.01, GeoMagic Studio 12 and ANSYS Workbench 14.0 were used to deal with magnetic resonance data of 27 healthy nulliparous volunteers'pelvic, and then three types of finite element models were developed:intact models, ELAPE models and individual ELAPE models. The maximum stress in non levator ani tissue under the same load were measured in three types of models, and levator ani 's maximal stresses were measured in intact model and individual ELAPE and their stress distributions under the same pressure were analyzed and compared. Results The maximal stresses of non-levator ani tissue were (1.963±0.061) MPa, (5.127±0.070) MPa and (4.703±0.110) MPa for intact model, ELAPE model and individual ELAPE model respectively. The maximal stress was lower in individual ELAPE model than that in ELAPE model, but which was higher than that of intact model (P<0.01). The high-stress zone was found at the joints with surrounding structures on both sides of intact model and ELAPE model. The high-stress zone was found in front of the joints with surrounding structures on both sides in individual ELAPE model. The maximal stresses of three types of models were found in front of both sides. In intact model levator ani 's maximal stress was (0.812 ± 0.042) MPa, which was higher than that of individual ELAPE model (0.719 ± 0.027) MPa (P<0.01). The high-stress zone of intact model was found in front of the joints on both sides. The maximal stress was showed at ventral ends on both sides. For the individual ELAPE model the high-stress zone was found at the anterior part of the levator ani muscle and the surrounding structure. The maximum stress appeared at the top end of the left and right sides. Conclusion This individual ELAPE is able to decrease the stress of non-levator ani tissue, which suggests that the risk of postoperative pelvic floor hernia is relatively reduced.%目的 应用有限元分析方法探讨个体化经肛提肌外腹会阴联合直肠切除(ELAPE)术治疗肛提肌裂孔下方的直肠肿瘤对术后盆底生物力学的影响.方法 利用MIMICS 10.01、GeoMagic Studio 12及ANSYS Workbench 14.0软件,处理27例健康未育女性志愿者的盆底核磁共振数据并建立3种盆底有限元模型:正常模型、ELAPE模型和个体化ELAPE模型,测量3种模型在相同载荷作用下非肛提肌组织内的最大应力及正常模型与个体化ELAPE模型肛提肌内的最大应力,并观察应力分布.结果 正常模型、ELAPE模型和个体化ELAPE模型的非肛提肌组织内最大应力分别为(1.963±0.061)MPa、(5.127±0.070)MPa和(4.703±0.110)MPa,个体化ELAPE模型最大应力低于ELAPE模型,但高于正常模型(均P<0.01);前两者的高应力区出现在左右两侧与周围结构的连接处,后者的高应力区出现在左右两侧与周围结构连接处的前部,三者最大应力均出现在左右两侧的最前端.正常模型肛提肌内最大应力为(0.812±0.042)MPa,高于个体化ELAPE模型的(0.719±0.027)MPa(P<0.01);前者的高应力区出现在左右两侧与周围结构连接处的前部,最大应力出现在左右两侧的最前端;后者的高应力区出现在剩余肛提肌与周围结构连接处的前部,最大应力出现在左右两侧最前端的上部.结论 该个体化ELAPE术对于盆底非肛提肌组织内的应力有降低作用,在一定程度上能够降低术后发生盆底疝的风险.

摘要： 目的:比较动态对比增强磁共振(DCE-MRI)三种血流动力学模型定量灌注参数鉴别诊断富细胞型子宫平滑肌瘤的价值.方法:连续收集2015年9月至2016年9月绍兴市人民医院经病理学检查证实的子宫平滑肌瘤患者64例(普通型30例,富细胞型13例,退变型21例).所有患者术前行盆腔DCE-MRI扫描,应用Reference Region(RR)模型、Extended Tofts(ET)模型和Exchange(EC)模型测量子宫平滑肌瘤的定量灌注参数值,对比分析子宫平滑肌瘤不同病理类型间定量灌注参数的差异,并采用受试者工作特征(ROC)曲线评价其诊断富细胞型子宫平滑肌瘤的效能.结果:富细胞型子宫平滑肌瘤RR模型中容量转运常数(Ktrans)、速率常数(Kep),ET模型中Ktrans、Kep、血管间隙容积分数(Vp),EC模型中血管外细胞外间隙容积分数(Ve)、血浆流量(Fp)值高于普通型子宫平滑肌瘤(均P0.05).ROC曲线分析结果显示,ET模型中的Ktrans诊断富细胞型子宫平滑肌瘤的效率较高,曲线下面积为0.929,敏感度和特异度分别为92.3%和83.7%;此外,EC 模型中的Fp、RR模型中的Ktrans和ET模型中的Kep诊断富细胞型子宫平滑肌瘤的效率也较高,曲线下面积分别为0.867、0.849和0.837,敏感度分别为91.7%、84.6%和92.3%,特异度分别为78.0%、76.0%和73.5%.结论:三种血流动力学模型定量灌注参数鉴别富细胞型子宫平滑肌瘤均具有较高价值,其中以ET模型中的Ktrans诊断富细胞型子宫平滑肌瘤的敏感度和特异度最高.%Objective: To assess the application of the dynamic-contrast enhanced magnetic resonance imaging (DCE-MRI) pharmacokinetics models in differential diagnosis of cellular uterine leiomyoma. Methods: Sixty four patients with uterine leiomyoma confirmed by surgery and pathology were enrolled in the study between September 2015 and September 2016, including 30 cases of classical leiomyoma, 13 cases of cellular leiomyoma and 21 cases of degenerative leiomyoma. All patients underwent DCE-MRI before surgery. Reference region (RR) model, extended tofts (ET) model and exchange (EC) model were used to quantitatively analyze DCE-MRI data,and their differences among different pathological types of uterine leiomyoma were observed. Receiver operating characteristic (ROC) curve was used to evaluate the efficiency of the quantitative perfusion parameters in differential diagnosis of cellular uterine leiomyoma. Results: The values of Ktrans(transfer constant), Kep(efflux rate constant) in RR model,Ktrans,Kep,Vp(blood plasma volume ratio) in ET model and Ve(plasma volume ratio),Fp(plasma flow)in EC model of cellular uterine leiomyoma were higher than those of classical type(P 0.05). ROC curves revealed that the Ktransof the ET model was more effective in diagnosing cellular uterine leiomyoma, the area under the curve (AUC) was 0. 929, and the sensitivity and specificity were 92.3% and 83.7%, respectively; meanwhile, the AUCs of Fpof the EC model, Ktransof the RR model and Kepof the ET model in diagnosis of cellular uterine leiomyoma were 0. 867, 0. 849 and 0. 837, the sensitivities were 91. 7%, 84.6% and 92. 3%, and the specificities were 78. 0%, 76. 0% and 73. 5%, respectively. Conclusions: Three pharmacokinetics models can be used in the differentiation of cellular uterine leiomyoma from other types of uterine leiomyoma. Ktrans of the ET model has higher sensitivity and specificity in differential diagnosis of cellular uterine leiomyoma.

摘要： 目的:评估动态增强磁共振成像(DCE-MRI)参照物模型定量参数与乳腺浸润性导管癌患者预后相关因素和乳腺癌分子病理分型之间的相关性.方法:回顾性分析50例经病理学检查证实的浸润性导管癌患者的MRI和病理学检查资料,运用DCE-MRI参照物模型测量药代动力学定量参数,包括病灶相对肌肉的容量转运常数(RR Ktrans)、病灶的速率常数(Kep)、病灶的容量转运常数与肌肉的血管外细胞外间隙容积比值(Ktrans/Ve),分析上述定量参数与乳腺癌患者预后相关因素和乳腺癌分子病理分型之间的相关性.结果:组织学分级为3级的病灶的平均RR Ktrans和Kep值高于组织分级为1 ~2级的病灶(均P <0.05);雌激素受体(ER)阴性者和孕激素受体(PR)阴性者的平均RR Ktrans值和Kep值分别高于ER阳性者和 PR 阳性者(均 P <0.05).三阴性乳腺癌患者的 RR Ktrans和 Kep高于Luminal型乳腺癌患者(均P<0.05).结论:DCE-MRI参照物模型所得定量参数RR Ktrans和Kep有助于预测乳腺癌的预后和鉴别乳腺癌的分子病理分型.%Objective: To investigate the association of parameters in dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) using reference region model with prognostic factors and molecular subtypes of breast cancer. Methods: MRI and pathological data of 50 patients with pathologically confirmed invasive ductal carcinoma of the breast were retrospectively analyzed. Reference region model was applied to analyze pharmacokinetic quantitative parameters including volume transfer constant (RR Ktrans), rate constant (Kep) and the ratio of Ktransto extracellular space volume (Ktrans/ Ve). The associations of the above parameters with prognostic factors and molecular subtypes of breast cancer were analyzed. Results:RR Ktransand Kepwere significantly higher in patients of histological grade 3 compared with those of histological grade 1 &2 (all P <0.05); and the patients with estrogen receptor (ER)-negative and/or progesterone receptor (PR)-negative also had higher RR Ktransand Kepthan those with ER-positive or PR-positive (all P<0.05). For immunohistochemistry, RR Ktransand Kepwere significantly higher in triple negative breast cancer compared with luminal type breast cancer (all P<0.05). Conclusion: High RR Ktransand Kepare associated with poor prognosis of breast cancer,and which can also be used to distinguish molecular subtypes of breast cancer.

摘要： 抗核抗体（ANA）是多种自身免疫病（AID）特别是ANA相关的风湿性疾病（AARD）诊断的重要血清标志物。以HEp－2细胞为基质的间接免疫荧光（ IIF）法是ANA检测的重要方法，被美国风湿病学会（ACR）、欧洲自身免疫标准化促进会（EASI）等专业学会推荐为ANA检测的参考方法。2014年8月28日在巴西圣保罗举行的ANA荧光模型国际共识第一次会议上，来自15个国家的66位专家研讨形成ANA HEp－2细胞间接免疫荧光染色模型命名标准化国际共识。2015年9月22日在德国德累斯顿召开的ANA荧光模型国际共识第二次会议上形成了ANA结果报告方式国际共识。认真解读和推广ANA HEp－2细胞间接免疫荧光模型及其结果报告标准化国际共识有助于提高我国ANA检测质量和充分发挥ANA在AID临床诊疗中的价值。（中华检验医学杂志，2016，39：804－810）%Antinuclear antibodies ( ANA) are important serum markers in the diagnosis of a variety of autoimmune diseases ( AID ) , especially ANA-associated rheumatic diseases ( AARD ) . Indirect immunofluorescence assay using HEp-2 cells as substrate is an important method for the detection of ANA , which is considered as the reference method for ANA detection by the American College of Rheumatology ( ACR) and the European Autoimmunity Standardization Initiative ( EASI ) . The first meeting for the International Consensus on Antinuclear antibody Pattern ( ICAP) was held in Sao Paulo , Brazil, on August 28, 2014.During the conference , a consensus on the nomenclature of staining patterns observed in the antinuclear antibody ( ANA) indirect immunofluorescence test on HEp-2 cells was established by 66 experts representing 15 countries. The second meeting for the ICAP was held in Dresden , Germany, on 22 September 2015, and achieved a consensus regarding the reporting ANA test results .It is helpful to improve the quality of ANA testing in China and give full play to the value of ANA in the clinical diagnosis and treatment of AID , by careful interpretation and promotion of the international consensus on standardized indirect immunofluorescence nomenclature of antinuclear antibody HEp -2 cell patterns and reporting ANA results.

摘要： 目的 应用物理方法构建正常人肝细胞株7702细胞的低氧饥饿自噬模型,为研究细胞自噬对肝脏功能的影响机制奠定基础.方法 选用7702细胞,使用完全DMEM培养基,在37°C和5％ C02培养箱中培养(正常对照组),使用Binder三气培养箱,设置温度为37°C,C02浓度为5％、02浓度为0.3％来提供缺氧环境,使用无血清DMEM培养细胞来诱导饥饿,将其分为低氧饥饿6、12、18和24 h4个时间组.用Western Blot检测正常对照组和不同时间点实验组的Beclin-1、Atg5以及LC3蛋白表达水平的变化;通过实时荧光定量PCR法检测各组Beclin-1 mRNA、Atg5 mRNA的表达;转染LC3质粒后利用免疫荧光法观察各组细胞发生自噬的情况.计量资料多组间比较采用方差分析,进一步两两比较采用LSD-t检验;计数资料组间比较采用x2检验.结果 低氧饥饿处理6h组的Beclin-l、Atg5及LC3的蛋白表达量高于正常对照组及其他处理组;低氧饥饿6h组较其他各组的Beclin-1mRNA和Atg5 mRNA的表达量显著增高,且其增高最显著,差异均有统计学意义(P值均＜0.05);低氧饥饿组自噬小体数量均高于正常对照组,其中低氧饥饿6h组的自噬小体数量最高,差异均有统计学意义(P值均＜0.05).结论 通过物理方法构建的低氧饥饿可以诱导7702细胞发生自噬,并且在低氧饥饿6h时,细胞发生自噬最为明显.

摘要： 本发明公开了一种肺腺癌生物标志物筛选、预后模型构建及生物学验证的新方法，其方法为：步骤一、对原始的基因表达矩阵进行数据的预处理；步骤二、在经过预处理后得到的数据上进行生物标志物筛选；步骤三、使用经过上述多步骤特征选择的肺腺癌预后标志物构建预后模型；步骤四、建模之后对选出的标志物进行生信分析。有益效果：使用线性支持向量机模型验证45个基因标志物，利用五折较差验证的AUC和ACC作为模型评估指标，最终的结果为AUC＝0.98，ACC＝0.92。该结果明显优于大多数传统基因标志物选择方法，本发明不仅能找出与肺癌相关的标志物，准确预测肺癌患者的生存期，同时还能发掘与肺腺癌发生相关的通路，探究肺腺癌的发生机制。

摘要： 本发明涉及体内筛选对肿瘤细胞生物学有影响生物大分子的动物模型，具体的涉及一些对肿瘤血管生成有影响的基因或蛋白，无法通过体内试验证明其对肿瘤生物学功能有影响，利用此模型可以有效屏蔽新生血管影响肿瘤生长这一干扰因素。本发明对现有肿瘤血管生成因子和抑制因子的进一步功能的研究提供可靠模型，为他们的临床应用提供基础，具有重要的意义。

摘要： 在生物学信息利用系统(100)中，测量系统(110)包括测量部(111)，测量被检者的生物学信息；时钟，检测出测量了生物学信息的测量时刻；通信部(12)，向服务器(120)发送包含测量时刻的生物学信息；服务器(120)包括从多个测量系统(110)中接收多个生物学信息的通信部(121)，存储生物学信息的生物学信息存储部(126)，根据在生物学信息存储部(126)中存储的多个生物学信息，生成表示生物学信息的地理分布或生物学信息的地理分布的时间推移的附加价值信息的附加价值信息生成部(123)，将所生成的附加价值信息提供给测量系统(110)和服务提供目的地的PC(130)与便携式电话机(110)的通信部(121)，PC(130)和便携式电话机(120)将所提供的附加价值信息提示输出给使用者。

摘要： 本发明公开了一种肺腺癌生物标志物筛选、预后模型构建及生物学验证的新方法，其方法为：步骤一、对原始的基因表达矩阵进行数据的预处理；步骤二、在经过预处理后得到的数据上进行生物标志物筛选；步骤三、使用经过上述多步骤特征选择的肺腺癌预后标志物构建预后模型；步骤四、建模之后对选出的标志物进行生信分析。有益效果：使用线性支持向量机模型验证45个基因标志物，利用五折较差验证的AUC和ACC作为模型评估指标，最终的结果为AUC＝0.98，ACC＝0.92。该结果明显优于大多数传统基因标志物选择方法，本发明不仅能找出与肺癌相关的标志物，准确预测肺癌患者的生存期，同时还能发掘与肺腺癌发生相关的通路，探究肺腺癌的发生机制。

摘要： 本发明涉及体内筛选对肿瘤细胞生物学有影响生物大分子的动物模型，具体的涉及一些对肿瘤血管生成有影响的基因或蛋白，无法通过体内试验证明其对肿瘤生物学功能有影响，利用此模型可以有效屏蔽新生血管影响肿瘤生长这一干扰因素。本发明对现有肿瘤血管生成因子和抑制因子的进一步功能的研究提供可靠模型，为他们的临床应用提供基础，具有重要的意义。

摘要： 本发明公开了蛋白酶清除口腔色素生物膜的分子生物学模型及其制备方法，本发明在QCM-D或SPR的芯片表面形成自组装的蛋白膜，建立起了口腔蛋白质生物膜的体外模型；随后加入常用食用色素化合物，在QCM和SPR上原位、实时和动态监测色素与生物芯片上的蛋白质的吸附和解吸附反应，建立牙和修复材料表面的着色生物膜模型，研究色素与蛋白质之间相互作用的机制，探索色素导致人然牙和人工材料表面发生着色的机理。进而通过蛋白酶(如木瓜蛋白酶等)，催化降解吸附的蛋白/色素膜，考察不同实验条件下生物蛋白酶降解色素的效率，探索不同实验条件下生物蛋白酶的生物活性，从而可以筛选出安全、有效的生物蛋白酶水解色素生物膜的最佳实验条件。

摘要： 浓缩膜，其用于浓缩生物学粒子，上述浓缩膜包含亲水性复合多孔质膜，上述亲水性复合多孔质膜具备：多孔质基材、和被覆上述多孔质基材的至少一个主面及空孔内表面的亲水性树脂，上述亲水性复合多孔质膜的膜厚t(μm)与利用掌上气孔计(palm porometer)测得的平均孔径x(μm)之比t/x为50～630。生物学粒子50的浓缩设备10，其具备：壳体20，其具有入口21及出口22，并且构成为包含生物学粒子50及水的被处理液40通过入口21与出口22的压差从入口21被注入并从出口22被排出；浓缩膜30，其是在壳体20内以隔开入口21与出口22的方式设置且不吸附生物学粒子50的亲水性多孔膜，上述浓缩膜30使排出液42从入口21侧的面透过至出口22侧的面，上述排出液42是自被处理液40减少了生物学粒子50的浓度而得的液体；和浓缩空间部24，其是壳体20内的浓缩膜30的上游侧的空间，且用于收纳浓缩液41，上述浓缩液41是利用浓缩膜30而自被处理液40增加了生物学粒子50的浓度而得的液体。

摘要： 本发明公开了一种高盐废水生物学脱盐装置及生物学脱盐方法，本发明高盐废水生物学脱盐装置包括多个脱盐单元，所述的脱盐单元包括废水输入管道、废水输出管道、充氧泵、中转池、检测池和多行多列脱盐池，脱盐池中均设置有浮板，浮板上设置有用于定植西洋海笋的定植杯，本发明高盐废水生物学脱盐方法，采用前述的高盐废水生物学脱盐装置，将废水排入脱盐单元的脱盐池中，并在定植杯中定植西洋海笋，将脱盐达标的废水从脱盐池中排出。本发明通过在废水池中设置浮板和定植杯，通过种植西洋海笋来吸收废水的盐，具有较好的脱盐效果，并且成本低无二次污染，还能绿化环境。

摘要： 本发明涉及基于超小型自组装肽的有机凝胶及乳剂。其还涉及用于制备此类有机凝胶及乳剂的方法以及所述有机凝胶及乳剂在生物学和非生物学应用中的用途。

摘要： 公开用于利用获自基于反射的光谱测量系统的反射测量结果估计生物物理学模型生物学参数向量的系统和方法。本方法利用半经验生物物理学模型来描述皮肤性质和估计反射光谱，和利用计算效率的基础向量来减少估计的和测量的反射光谱的维数。算法的混合用于产生初始参数组，其进而利用迭代的基于对照的技术被进一步细化，其中将得自测量光谱的参数之间的差错与从估计光谱计算的参数进行比较。然后处理这些差错以对初始参数组产生小δ。重复该过程直到估计的虚拟生物学参数和测量的虚拟生物学参数之间的差错降到零或另外在预先限定的阈水平之下。