摘要：
目的:观察中药复方天香胶囊对晕动病模型大鼠行为学与前庭核c-fos蛋白表达的影响.方法:选择雄性SD大鼠并将其随机分为正常组、模型组、阳性药组、天香胶囊低、中、高剂量组.灌胃预给药3天后,采用双轴旋转刺激法建立大鼠晕动病模型,通过晕反应指数评分、自发活动实验、异嗜高岭土实验检测各组大鼠行为学变化,Western blotting法与免疫组化法检测各组大鼠前庭核c-fos蛋白的表达.结果:与正常组大鼠相比,模型组大鼠晕反应指数和高岭土摄入量均显著增高,自发活动总路程与次数均显著减少,前庭核c-fos蛋白表达水平显著增高;与模型组相比,天香胶囊可显著降低晕动病模型大鼠晕反应指数与高岭土摄入量,提高自发活动总路程与活动次数,降低大鼠前庭核组织c-fos蛋白表达.结论:天香胶囊可有效改善晕动病模型大鼠的行为学变化,其作用机制可能与下调前庭核c-fos表达有关,提示降低前庭核兴奋性可能是天香胶囊抗晕动病的内在机制之一.%Objective:To investigate the effects of Tianxiang capsule on the behavior and c-fos expression in the vestibule nucleus of rats with motion sickness.Methods:Male SD rats were randomly divided into six groups,including normal group,model group,scopolamine group,low-dose,mid-dose and high-dose Tianxiang capsule groups.Prior to biaxial rotation stimulus inducing rats motion sickness model,the drugs were intragastrically administrated for 3 days.The behavioral changes of rats were evaluated by the motion sickness index,the spontaneous activity test and Kaolin pica.Meanwhile,the expression of c-fos was detected by Western Blotting and Immunohistochemistry.Results:Compared with the normal group,the motion sickness index and Kaolin intake were increased,total traveling distance and frequency were decreased,the c-fos expression was up-regulated significantly in the model group.Compared with the model group,the motion sickness index and Kaolin intake were decreased,total traveling distance and firequency were increased,the c-fos expression was down-regulated significantly in Tianxiang capsule groups.Conclusions:Tianxiang capsule could effectively improve the behavioral changes of rats with motion sickness,which might be related to the down-regulation of c-fos in vestibular nucleus.Inhibiting the excitability of vestibular nucleus may be the pharmacological mechanisms of Tianxiang capsule in motion sickness.
