摘要：
To determine the effect of culture medium, temperature, and time on pollen germination rates, differ-ent materials: Magnolia biondii, Magnolia 'Changhua' , Magnolia 'Danxin' and Magnolia cylindrica were used to study variation in pollen viability. Treatments of sucrose, H3BO3 and PEG-4000 were used to determine the effects on pollen germination with orthogonal experiments alone as well as for interactions. Results showed that (1) Different individuals need certain concentrations of the culture medium: Magnolia biondii was 50 g·L-1 sucrose+50 mg·L-1 H3BO3+200 g·L-1 PEG-4000;Magnolia 'Changhua' was 100 g·L-1 sucrose+200 mg·L-1 H3BO3 + 200 g·L-1 PEG-4000; Magnolia 'Danxin' was 100 g·L-1 sucrose + 100 mg·L-1 H3BO3 + 150 g·L-1 PEG-4000 and Magnolia cylindrica was 50 g·L-1 sucrose + 100 mg·L-1 H3BO3+ 250 g·L-1 PEG-4000. (2) For pollen viability, 25 °C was the best temperature, higher temperatures inhibited pollen viability. (3) At 9 h, Magnolia biondii (71.19％) and Magnolia cylindrica (59.82％) had the highest germination rates (P<0.05);whereas, at 12 h Magnolia 'Changhua' (44.50％) and Magnolia 'Danxin' (23.43％) had the highest germina-tion rates(P<0.05).%以望春玉兰Magnolia biondii,长花玉兰Magnolia'Changhua',丹馨玉兰Magnolia'Danxin',黄山木兰Mag-nolia cylindrica等4种木兰属Magnolia植物为试验材料,运用不同质量浓度蔗糖、 硼酸、 分子量为4000的聚乙二醇(PEG-4000)对花粉进行单因子及正交试验处理,研究不同培养基、 培养温度及培养时间对4种植物花粉萌发率的影响.结果表明:①蔗糖、 硼酸、PEG-4000对4种木兰属植物花粉萌发均有显著影响(P<0.05).不同植物所需适宜的离体培养基组分质量浓度不同,望春玉兰为50 g·L-1蔗糖+50 mg·L-1硼酸+200 g·L-1PEG-4000;长花玉兰为100 g·L-1蔗糖+200 mg·L-1硼酸+200 g·L-1PEG-4000;丹馨玉兰为100 g·L-1蔗糖+100 mg·L-1硼酸+150 g·L-1PEG-4000;黄山木兰为50 g·L-1蔗糖+100 mg·L-1硼酸+250 g·L-1PEG-4000.②4种植物花粉萌发最佳培养温度均为25°C,温度过高会抑制花粉萌发.③随着处理时间的延长,4种植物花粉萌发率增加,但达到最大萌发率所需时间有所不同,其中望春玉兰和黄山木兰在培养9 h时萌发率最高,萌发率分别为71.19％和59.82％,而长花玉兰和丹馨玉兰在培养12 h时萌发率最高,萌发率分别为44.50％和23.43％.
