散射测浊法和比浊法

摘要： 目的 探讨血清S-100B与C反应蛋白( CRP)在脑外伤患者病情严重程度及预后评估中的临床价值.方法 选取开封市中医院2015年10月至2017年6月收治的脑外伤患者108例(脑外伤组)以及同期健康体检者30例(对照组)为研究对象,采用格拉斯哥昏迷评分( GCS)将脑外伤患者分为重度损伤者(重度组,26例),中度损伤者(中度组,58例),轻度损伤者(轻度组,24例);采用酶联免疫吸附试验( ELISA)检测所有对象血清S-100B,免疫比浊法测定CRP水平,比较不同组别血清S-100B和CRP水平,分析血清S-100B、CRP在脑外伤患者病情严重程度及预后评估中的临床价值.结果 对照组血清 S-100B 水平为(0. 36 ± 0. 12)μg/L,CRP水平为(1. 35 ± 0. 43)mg/L,与脑外伤组中三组的入院24 h血清S-100B、CRP差异均有统计学意义(F=175. 45、475. 42,均P＜0. 01);脑外伤组中重度组、中度组、轻度组24 h、3 d、7 d、14 d血清S-100B水平、CRP水平差异均有统计学意义( F=89. 62、62. 45、42. 55、43. 22、183. 36、71. 12、70. 07、28. 99,均 P＜0. 01);脑外伤患者预后良好的有92 例,预后较差的有16 例,预后良好组血清 S-100B、CRP 水平分别为(0.42 ±0.13)μg/L、(39.42 ±6.18)mg/L,均低于预后较差组的(1.73 ±0.46)μg/L、(68.42 ±7.54)mg/L,差异均有统计学意义(t=22. 93、16. 75,均P＜0. 01).结论 通过检测血清S-100B、CRP水平能评估脑外伤患者的病情严重程度,判定患者的预后.

摘要： 目的 分析应用超声分散比浊法处理的BACTEC MGIT 960(简称“MGIT 960”)培养阳性的液体培养物菌液标本用于分枝杆菌药物敏感性试验(简称“药敏试验”)的价值.方法 搜集2018年1-12月西安市胸科医院经MGIT 960液体分枝杆菌培养阳性的标本,共计1086份.其中,818份菌液标本进行MGIT 960液体药敏试验,包括MGIT 960推荐方法处理的菌液标本100份、超声分散比浊法处理的菌液标本718份;268份菌液标本进行比例法药敏试验,包括采用传统磨菌法处理的菌液标本30份、超声分散比浊法处理的菌液标本238份.与传统菌液标本处理方法比较,分析超声分散比浊法处理后的菌液标本用于MGIT 960液体药敏试验和比例法药敏试验的效果.结果 超声分散比浊法处理后的0.6～1.0麦氏单位菌液标本进行MGIT 960液体药敏试验报告结果的时间主要集中在8～10 d,而MGIT 960法处理后的菌液标本报告结果时间则较均匀分布在5～14 d.浊度为0.6、0.7、0.8、0.9、1.0麦氏单位菌液标本系统检测X200(标本活菌量少)报告率[分别为0.00％、0.00％、0.00％、0.72％(1/138)、1.31％(2/153)]均低于MGIT 960菌液处理法[11.00％％(11/100)],差异均有统计学意义(x2值分别为11.41、9.94、12.43、12.79、11.28,P值均＜0.01).进行二线抗结核药物比例法药敏试验时,增菌6～7 d后,应用超声分散比浊法处理的菌液标本基本可以达到目标浊度,而传统磨菌法处理的标本在增菌第7天时仅有80％达到目标浊度.应用超声分散比浊法处理后的浊度为0.7、0.8、0.9、1.0麦氏单位的菌液标本,药敏试验成功率分别为82.61％(19/23)、82.69％(43/52)、94.00％(47/50)、96.00％(48/50),与传统磨菌法处理的菌液标本药敏试验成功率[83.33％ (25/30)]相近,差异均无统计学意义(x2值分别为0.01、0.01、2.37、3.77,P值均＞0.05).结论 应用超声分散比浊法处理MGIT 960液体培养阳性的菌液标本,可提高MGIT 960液体药敏试验成功率,缩短比例法药敏试验时间.

摘要： 目的 探讨血清超敏C反应蛋白(hs-CRP)、癌胚抗原(CEA)、糖类抗原724(CA724)、糖类抗原199(CA199)检测对胃癌及其相关病变的早期诊断价值.方法 采用免疫比浊法和电化学发光法对就诊于新疆自治区人民医院胃癌病人86例、中重度异型增生的病人34例、萎缩性胃炎的病人63例、健康人(对照组)70例血清hs-CRR、CEA、CA724和CA199水平进行检测.结果 胃癌组血清hs-CRP、CEA、CA724和CA199水平及阳性检出率均高于萎缩性胃炎组及对照组(P0.05).胃癌病人低分化组血清hs-CRP、CA724和CA199水平高于高分化组及中分化组(P<0.05);中分化组血清hs-CRP水平高于高分化组(P<0.01).4种指标联合检测的敏感性和准确性均高于单项检测(P<0.05).结论 联合检测血清hs-CRP、CEA、CA724和CA199对胃癌的早期诊断、治疗及预后评估有重要价值.

摘要： 目的 利用脂肪乳模拟不同浓度三酰甘油(TG)的脂血样本,分析不同浓度TG对常见免疫比浊法生化检测项目的影响.方法 通过向基质血清中加入不同量的脂肪乳,模拟5个不同浓度T G的脂血样本(M 1组为0μL脂肪乳、20μL生理盐水,M 2组为5μL脂肪乳、15μL生理盐水,M 3组为10μL脂肪乳、10μL生理盐水,M 4组为15μL脂肪乳、5μL生理盐水,M 5组为20μL脂肪乳、0μL生理盐水).使用全自动生化分析仪检测不同组中免疫比浊法检测项目,随后通过高速离心(12000 r/min离心10 min),检测离心前后免疫比浊项目测定值的变化.结果 随着TG浓度的升高,纤维蛋白原(Fn)检测值逐步升高,M1～M5组Fn检测值比较,差异均有统计学意义(P0.05).M1组超速离心后所有检测指标与离心前比较,差异均无统计学意义(P>0.05);M2组超速离心后TG水平与离心前比较,M3组超速离心后TG、Fn、RBP水平与离心前比较,M4、M5组超速离心后TG、Fn、RBP、PA与离心前比较,差异均有统计学意义(P<0.05).结论 不同浓度的TG会对不同的免疫比浊项目检测造成干扰,超高速离心可减轻这种干扰,但不能完全消除.检验人员与临床医生均应知晓脂血对生化检测结果的影响,为更好地解读检验报告,更准确地诊断病情、判断预后奠定理论基础.

摘要： Objective To analysis the effect of lens opacity on the measurement of retinal vessel oxygen saturation.Methods This was a cross sectional study.Forty four eyes of 44 patients with different degrees of lens opacity were enrolled.There were 23 males and 21 females.The patients aged from 48 to 84 years,with the mean age of(71.8± 10.3) years.The mean best corrected visual acuity was 0.65±0.22.The mean intraocular pressure was (14.2± 4.3) mmHg (1 mmHg=0.133 kPa).The mean equivalent spherical degree was (-0.05 ± 2.10) D.The opitical quality analysis system was applied to measure intraocular objective scattering index (OSI) caused by lens opacity.According to the OSI,the opacity of lens was divided into four groups.Patients with OSI value ＜ 1.0 was grouped to level 1,which indicated that the lens were basically transparent;patients with OSI value between 1.0 and 3.0 was grouped to level 2,which indicated early cataract;patients with OSI value between 3.0 and 7.0 was grouped to level 3,which indicated progressive cataract;patients with OSI value ＞ 7.0 was grouped to level 4,which indicated the mature stage of cataract.The retinal oximeter Oxymap T1 was used to capture the fundus images under different wavelengths.Pearson correlation analysis was used to analyze the correlation between retinal oxygen saturation and age,intraocular pressure,equivalent spherical degree and OSI.One way ANOVA was used to analyze the difference of retinal oxygen saturation among groups.Results The mean retinal arterial oxygen saturation,venous oxygen saturation and arteriovenous difference was (90.70± 6.46)％,(47.34 ± 13.51)％,(43.36 ± 10.09)％,respectively.The correlations of retinal arterial oxygen saturation,venous oxygen saturation and arteriovenous difference with age,intraocular pressure,equivalent spherical degree was not statistically significant (all P＞0.05).The retinal arterial oxygen saturation and venous oxygen saturation was negatively correlated with OSI (r=-0.462,-0.500;P=0.002,0.001),the arteriovenous difference and OSI was positively correlated (r=0.373,P=0.013).According to lens opacity,there were 11 eyes in level 1,9 eyes in level 2,14 eyes in level 3,10 eyes in level 4.There were significant differences of retinal artery and venous oxygen saturation among different lens opacity levels (F=5.340,4.710;P=0.003,0.007);meanwhile,the arteriovenous difference was not significantly different (F=2.048,P=0.123).The retinal arterial oxygen saturation and venous oxygen saturation was significantly lower in the level 4 lens opacity group than any other three groups (all P＜0.05),but there was no statistically significant difference among level 1 to level 3 lens opacity group.Conclusion The effect of lens opacity of level 1 to level 3 is limited on the measurement of retinal oxygen saturation,but level 4 lens opacity will cause decrease of retinal artery and venous oxygen saturation.%目的 观察不同程度晶状体混浊对视网膜血氧饱和度测量结果的影响.方法 横断面研究.不同程度晶状体混浊的44例患者44只眼纳入研究.其中,男性23例,女性21例.年龄48～84岁,平均年龄(71.8±10.3)岁.患眼平均最佳矫正视力0.65±0.22;平均眼压(14.2±4.3) mmHg(1 mmHg=0.133 kPa);平均等效球镜度数(-0.05±2.10)D.采用视觉质量分析系统测量患眼眼内客观散射指数(OSI),并根据OSI对晶状体混浊程度进行分级.OSI＜1.0为1级,表示晶状体基本透明;1.0≤OSI＜3.0为2级,表示早期白内障;3.0≤OSI＜7.0为3级,表示进展期白内障;OSI＞7.0为4级,表示成熟期白内障.采用视网膜血氧饱和度分析仪拍摄患眼不同波长光束下的眼底像,应用配套软件自动计算得出视网膜动脉、静脉血氧饱和度值.采用Pearson相关分析法对视网膜血氧饱和度与年龄、眼压、等效球镜度数、OSI之间的相关性进行分析.对比分析不同程度晶状体混浊患眼之间的视网膜血氧饱和度差异.结果 患眼平均视网膜动脉、静脉血氧饱和度及动静脉血氧饱和度差值分别为(90.70±6.46)％、(47.34±13.51)％、(43.36±10.09)％.相关性分析结果显示,视网膜动脉、静脉血氧饱和度及动静脉血氧饱和度差值与年龄、眼压、等效球镜度数均无线性相关性(P＞0.05).视网膜动脉(r=-0.462,P=0.002)、静脉血氧饱和度(r=-0.500,P=0.001)与OSI呈负相关;动静脉血氧饱和度差值与OSI呈正相关(r=0.373,P=0.013).44只眼中,晶状体混浊程度为1级11只眼,2级9只眼,3级14只眼,4级10只眼.晶状体混浊程度不同分级患眼视网膜动脉、静脉血氧饱和度比较,差异有统计学意义(F=5.340、4.710,P=0.003、0.007);动静脉血氧饱和度差值比较,差异无统计学意义(F=2.048,P=0.123).4级晶状体混浊患眼的视网膜动脉、静脉血氧饱和度较1～3级晶状体混浊患眼明显降低,差异均有统计学意义(P＜0.05);1、2、3级晶状体混浊患眼之间视网膜动脉、静脉血氧饱和度比较,差异均无统计学意义(P＞0.05).结论 1～3级晶状体混浊对视网膜血氧饱和度测量结果无明显影响,4级晶状体混浊可致视网膜动脉、静脉血氧饱和度下降.

摘要： Objective To develope rate nephelometry (RN) for accurate determination of different types of polysaccharides (PSs) in 23-valent pneumococcal polysaccharide vaccine (PPV23) .Methods The 23-valent PS references and anti-sera were prepared .The RN for determination of different types of PSs in PPV23 was developed .The linearity ,precision ,accuracy ,and specificity of the developed RN were validated .The application research was carried out using the RN within the quality control range . Results Under the designed condition ,when PS concentrations were in a range of 1 .0-5 .0 μg/ml ,a good linear correlation was shown between PS concentration and its corresponding instrumental response value with coefficient of determination > 0 .99 . The method had good precision and accuracy . The coefficients of variation of intra-and inter-assay were 0 .50％-4 .98％ and 2 .81％-9 .52％ ,respectively , and the adding recovery rates were 90 .3％-118 .8％ . The specificity of the method was good as the pneumococcal anti-sera only reacted with homotype PS ,not heterotype PSs .The application research showed that the RN could accurately detect different types of PSs in PPV 23 .Conclusion The developed RN can effectively control the internal quality of PPV 23 ,and can be applied to determination of different types of PSs in PPV23 .%目的 建立准确检测23价肺炎球菌多糖疫苗(23-valent pneumococcal polysaccharide vaccine,PPV23)各型多糖(polysaccharide,PS)含量的速率散射比浊法(rate nephelometry,RN).方法 制备23价PS参考品和抗血清,建立检测PPV23各型PS含量的RN,并对建立的RN进行线性、精密度、准确度和特异性验证.采用建立的RN在质量控制范围内进行应用性研究.结果 在设定的条件下,当PS参考品质量浓度为1.0～5.0μg/ml时,PS浓度与相应响应值呈良好的线性关系,决定系数>0.99.该法的精密度和准确度良好,批内和批间变异系数分别为0.50％ ～4.98％ 和2.81％ ～9.52％,加样回收率为90.3％ ～118.8％.该法具有良好的特异性,肺炎球菌抗血清仅与同型PS发生特异性反应,不与其他型PS发生交叉反应.应用性研究显示,在质量控制范围内,该法可准确检测放置不同时间的PPV23各型PS含量.结论 建立的RN可有效控制PPV23的内在质量,可用于PPV23各型PS含量检测.

摘要： 肝素诱导的血小板减少症（ HIT）是严重的由抗体介导的肝素副作用，实验室检查有两个主要类型，包括功能分析和HIT抗体检测。功能分析试验中的5-羟色胺释放试验（ SRA）和肝素诱导的血小板活化试验（ HIPA）有高度灵敏性和特异性，是公认的参考方法，缺点是操作复杂且耗时长，不能作为常规检查手段。 HIT抗体检测方法中的ELISA、免疫比浊法、化学发光法和侧流免疫分析均有很高的诊断敏感性且可在普通实验室进行，这些试验与验前概率评分（4Ts评分）结合使用可有效进行HIT的排除诊断或诊断。（中华检验医学杂志，2016，39：795-800）%Heparin induced thrombocytopenia ( HIT ) is a severe side effect of heparin with antibody-mediation.Laboratory assays can be divided into two major categories, about functional assays and HIT antibodiesdetection.Thefunctional assays, such as the serotonin release assay ( SRA ) and heparin-induced platelet activationassay( HIPA) , are sensitive and specific for HIT.They arethe reference standard assays generally, but have thedeficiencies of complicated operation and time-consuming, and cannot be used as a routine examination. TheHIT antibodiesdetections, such as ELISA, immune turbidimetry assay, chemiluminescent assay and lateral flow immunoassay, have high diagnostic sensitivityandareavailable at routine laboratories.They can exclude the diagnosis of HIT or beused to diagnose HIT effectively combined with the pre-test probability score(4Ts score) of HIT.

摘要： 目的：探讨血红蛋白变异体对糖化血红蛋白（ HbA1c ）不同检测系统的影响。方法采用酶法（Norudia N HbA1c，SEKISUI）、免疫透射比浊法（oneHbA1c FS，DiaSys）、亲和层析高压液相色谱法（Ultra2，Trinity Biotech）、毛细管电泳法[Capillary 2 Flex Piercing（CFP）Sebia]和离子交换高压液相色谱法[HA8160，Arkary；VariantⅡTurbo（Ⅶ－T），Bio－Rad；VariantⅡTurbo 2．0（Ⅶ－T 2．0），Bio－Rad]7种检测系统测定正常样本和血红蛋白变异体样本HbA1c ，分析血红蛋白变异体对HbA1c检测结果的影响。结果对于正常样本，7种检测系统的相关性良好。对于（ HbE）杂合子，以（ CFP）为参比系统，Ⅶ－T的HbA1c结果分成高低两群，各检测系统的HbA1c结果超过6％比例范围为14．9％～73．1％。对于HbE纯合子，Ultra2检测出部分样本的糖化血红蛋白结果，CFP和Ⅶ－T 2．0未给出HbA1c结果。常见血红蛋白变异体中，J－Bangkok、Hb J－Bangkok、Hb J－Newyork、Hb G－Taipei和Hb G－Coushatta 样本的HbA1c结果偏倚大小不一。结论血红蛋白变异体对不同HbA1c检测系统有着不同的影响，CFP可提示常见血红蛋白变异体。（中华检验医学杂志，2015，38：470－474）%Objective To assess influences of hemoglobin variants on different HbA 1c measurement systems.Methods HbA1c values of normal samples and samples with hemoglobin variants were measured respectively byenzymatic assay (Norudia N HbA1c,SEKISUI), immunity transmission turbidity (oneHbA1c FS,DiaSys), boronate affinity HPLC(Ultra2,Trinity Biotech), capillary electrophoresis(Capillary 2 Flex Piercing,Sebia)and ion exchange HPLC(HA8160,Arkary;Variant Ⅱ Turbo(VⅡ-T),Bio-Rad;Variant ⅡTurbo 2.0 (VⅡ-T 2.0), Bio-Rad).HbA1c values from different hemoglobin variants and HbA1c measurement systems were analyzed .Results The HbA1c values from the 7 HbA1c measurement systems were well correlatedin normal samples .For HbE heterozygote , the HbA1c values from VⅡ-T were divided into 2 groups comparing with CFP , and HbA1c differences between CFP and other measurement systems are minor except for HA8160 and VⅡ-T.The HbA1c values of homozygous HbE were given by Ultra 2 but CFP and VⅡ-T 2.0.The differences of HbA1c values from samples with J-Bangkok are much higher than those from the samples with other hemoglobin variants .The differences of HbA1c values from samples with all kinds of hemoglobin variants(Hb J-Bangkok, Hb J-Newyork, Hb G-Taipei and Hb G-Coushatta)are dramatic for VⅡ-T.For rare Kurosaki, CFP can give a hint that there existshemoglobin variant while measuring HbA 1c. Conclusions Capillary Flex 2 Piercingcan well recognize common hemoglobin variants . Different hemoglobin variants have different influences on different HbA 1c measurement systems.The influences of J-Bangkok among HbA1c measurement systems are more evident than the other common hemoglobin variants .

摘要： 目的 考察能否用单克隆抗体(单抗)替代多克隆抗体(多抗)血清检测23价肺炎球菌多糖疫苗中各型多糖的含量.方法 使用8个血清型(2、3、4、6B、9N、17F、18C、23F)的小鼠抗肺炎球菌荚膜多糖单抗和丹麦国家血清研究所(Statens Serum Institut,SSI)的兔血清多抗,以速率散射免疫浊度法测定23价肺炎球菌多糖疫苗中相应血清型的多糖含量.通过重复性和专属性试验确定单抗的可用性.采用t检验对单抗和多抗测定结果进行比较.结果 各型单抗与多糖标准品反应标准曲线的相关系数均＞0.985 0.用单抗重复检测疫苗多糖3次,质量浓度为40.8～62.1 μg/ml,3次检测结果变异系数均＜8.00％.各型多糖回收率为81.6％～124.2％.分别用8个血清型单抗检测其余各型多糖含量,结果均低于或接近于检测下限.单抗与SSI多抗血清的检测结果差异均无统计学意义,t值为0.210 3～1.926 0,P值均＞0.05.结论 单抗检测重复性好、特异性高,与多抗血清检测结果相仿,因此,单抗可以替代SSI多抗血清用于测定23价肺炎球菌多糖疫苗中的多糖含量.%Objective To investigate whether polyclonal antibody sera could be replaced by monoclonal antibodies (Mabs) to determine contents of pneumococcal polysaccharides in a 23-valent pneumococcal polysaccharide vaccine (PPV23).Methods Mouse Mabs against polysaccharides of types 2,3,4,6B,9N,17F,18C and 23F,and rabbit sera from Statens Serum Institut (SSI)were used.The pneumococcal polysaccharides in PPV23 were determined by rate Nephelometry.Repeatability and specificity were analyzed to evaluate the usability of Mabs.The results with two kinds of antibodies were compared by t test.Results When Mabs to different serotypes reacted with standard polysaccharides,all correlation coefficients of standard curves were ＞0.985 0.The polysaccharides in PPV23 ranged from 40.8 to 62.1 μg/ml when determined 3 times by Mabs,and the coefficients of variability were ＜8.00％.The recovery rates of different serotypes of polysaccharides ranged from 81.6％ to 124.2％.Each Mab was used to test the other 22 serotypes of polysaccharides,the content levels were lower than or close to the lower limit of detection.No statistical difference was found between the results of Mabs and SSI sera,t values ranged from 0.210 3 to 1.926 0,and all P values were ＞ 0.05.Conclusion The determination results of polysaccharides with Mabs are good in repeatability and specificity,and are comparable to that of sera,therefore,the Mabs could replace SSI rabbit sera for determination of polysaccharides in PPV23.

摘要： Objective To evaluate the clinical value of serum free light chain kappa/lambda ratio (sFLC)in kidney damage of patients with chronic kidney disease (CKD).Methods According to the results of serum immune fixation electrophoresis (IFE) and serum free light chainκ/λratio,95 cases of patients with chronic kidney disease were divided into three groups:kappa light chain predominate positive group (group A)30 cases,lambda light chain positive group of 31 cases (group B),chronic renal insuffi-ciency in the control group (C group)34 cases and 30 cases of healthy physical examination were selected as control group (group D).Immunological transmission turbidimetry was used to detect serum cystatin C(Cys C),C-reactive protein(CRP),free blight chain kappa and lambda concentration levels.urine beta 2 microglobulin (β2-MG),trace albumin (MA)and 24 h urinary total pro-tein (24 h PRO)contents,serum creatinine (CREA)and urine N-acetyl-β-D glycosidase enzymes (NAG)activity were detected by enzymatic method.Results The serum CREA,CRP,Cys C and urine MA,β2-MG,24 h PRO concentration levels and NAG activity of group A,group B and group C were obviously higher than those of group D.Compared with group D,the difference was statisti-cally significant (P<0.01).The serum CRP,Cys C and urine MA,β2-MG,24 h PRO concentration levels and NAG activity of group A and group B were obviously higher than those of group C.The difference was statistically significant (P<0.01). Conclusion The measurement of serum free light chain kappa/lambda ratio in renal damage degree evaluation for patients with chronic kidney disease (CKD)has important clinical significance.%目的：探讨血清游离轻链κ/λ比值（sFLC）在慢性肾病（CKD）患者肾损伤中的诊断价值。方法根据血清免疫固定电泳（IFE）结果和免疫透射比浊法测定血清游离轻链κ/λ比值，将95例慢性肾病患者分为3组：κ轻链阳性组（A组）30例；λ轻链阳性组（B组）30例；慢性肾功能不全对照组（C组）34例。另选取30例健康体检者作为对照组（D组）。用免疫透射比浊法检测血清中胱抑素C（Cys C）、C反应蛋白（CRP）、游离轻链κ和λ浓度水平及尿液中β2微球蛋白（β2-MG）、微量清蛋白（MA）和24小时尿总蛋白（24 h PRO）水平；酶法检测血清肌酐（CREA）和尿液中N-乙酰-β-D葡萄糖苷酶（NAG）活性。结果 A 组、B组和C 组血清CREA、CRP、Cys C及尿液MA、β2-M、24 h PRO浓度水平和NAG活性均增高，与D组比较，差异有统计学意义（P＜0．01）。A组和B组血清CRP、Cys C及尿液 MA、β2-MG、24 h PRO浓度水平和NAG活性高于C组，差异有统计学意义（P＜0．01）。结论血清游离轻链κ/λ比值检测在CKD患者肾损伤程度评估中具有重要的临床意义。

摘要： 本发明涉及样本浓度测量技术领域，尤其涉及一种联合散射比浊法和透射比浊法的测量方法。它结合每个比浊法的随机误差ε和偏移f，分别得到两个比浊法的总误差TE，然后将两个总误差相比，若两个总误差不相同，则选择总误差小的测量方法作为准值，若两个总误差相同，则取散射比浊法与散射比浊法两者测量值的均值作为准值。这种测量方法测量准确性较高。

摘要： 本发明公开了一种用于提高胶乳增强免疫比浊法试剂检测准确度的方法，当胶乳增强免疫比浊法试剂为包被抗体检测抗原的胶乳增强免疫比浊法试剂，制备R2试剂时，成倍增加胶乳增强免疫比浊法试剂中的目的抗体的投料量；当胶乳增强免疫比浊法试剂为包被抗原检测抗体的胶乳增强免疫比浊法试剂，制备R2试剂时，成倍减少胶乳增强免疫比浊法试剂中的目的抗原的投料量。本发明的方法可以改善检测的临床相关性，提高胶乳增强免疫比浊法试剂的准确性，可以广泛应用于各项胶乳免疫比浊检测项目中。本发明还公开了一种胶乳增强免疫比浊法试剂。

摘要： 本发明提供了一种免疫散射比浊法的光源功率的校准装置及方法，包括集成于免疫散射比浊法探测仪中的光源模块、孔径光阑、光探测模块及计算模块，所述光源模块发出的光束沿着光路到达所述孔径光阑，并穿过所述孔径光阑形成偏离所述光路方向的混合光，所述光探测模块检测所述混合光的功率信息并发送给所述计算模块，所述计算模块根据所述混合光的功率信息判断所述光源的功率变化以得到校准系数，即刚开机测量与连续开机N小时后测量，两者测量结果的一致性，当仪器使用一段时间后温度变化导致光源功率变化，或长期使用后光源或电路老化导致光源功率变化，测量结果偏差的问题得以解决。

摘要： 本发明提供了散射比浊法检测微流控芯片及使用该芯片的生化免疫机，属于检测设备领域，包括本体、混合槽和反应小孔，本体上设有样本加液槽、样本定量槽和样本溢流槽，样本加液槽与样本定量槽连通，样本定量槽与样本溢流槽连通，本体上还设有稀释液加液槽、稀释液定量槽和稀释液溢流槽，稀释液加液槽与稀释液定量槽连通，稀释液定量槽与稀释液溢流槽连通，样本定量槽和稀释液定量槽均与混合槽连通，混合槽通过流道组合与反应小孔连通，反应小孔靠近本体圆心的一侧为平面，本体与反应小孔外圈对应位置为外凸的弧形。本发明有效的控制散射比浊法检测光线的发散，检测光强灵敏度提升，可用于比色法和透射比浊法进行检测。

摘要： 本发明公开了一种基于免疫散射比浊法的全自动检测装置及其方法，通过将采样针和两个试剂针，实现样本和双试剂针的单独加载，避免液体试剂间的交叉污染，提升检测准确度；各通道之间的加样和清洗是相互独立的，大大提高了整个检测装置的效率；反应杯可重复使用，不仅节约成本，而且实现了免疫散射比浊法的全自动检测设备的小型化智能化，具有更好的适用医院、小型诊所的检测需求；同时，采用磁场变换的底部螺旋涡流搅拌混匀及清洗技术，实现反应杯较佳的搅拌和清洗效果。

摘要： 一种多光谱散射及透射比浊法进行检测的分析仪测头，圆柱形测头轴线中央为圆柱体盲孔，即检测位，在盲孔靠近底部并在垂直于圆柱体轴线的截面上每隔30°圆心角均匀分布着12个通孔；在每隔120°圆心角通孔外端部均匀地安装着可见光、紫外光和红外光三组不同波长的冷光光源，并在与其直线相对应的通孔外端处分别设置光电转换接收器；在可见光、紫外光和红外光这三组不同波长的冷光光源两侧的通孔外端部分别安装光电转换接收器。本实用新型的优点是：1、可在可见光、紫外光和红外光三种波长光谱的范围里分别进行不同项目的检测或同一项目不同组分的透射光比浊法检测和散射光比浊法检测。2、结构紧凑，便于维修，测量精度高，造价低廉。

摘要： 本实用新型提供了散射比浊法检测微流控芯片结构，属于检测设备领域，包括本体、混合槽和反应小孔，本体上设有样本加液槽、样本定量槽和样本溢流槽，样本加液槽与样本定量槽连通，样本定量槽与样本溢流槽连通，本体上还设有稀释液加液槽、稀释液定量槽和稀释液溢流槽，稀释液加液槽与稀释液定量槽连通，稀释液定量槽与稀释液溢流槽连通，样本定量槽和稀释液定量槽均与混合槽连通，混合槽通过流道组合与反应小孔连通，反应小孔靠近本体圆心的一侧为平面，本体与反应小孔外圈对应位置为外凸的弧形。本实用新型有效的控制散射比浊法检测光线的发散，检测光强灵敏度提升，可用于比色法和透射比浊法进行检测。

摘要： 本实用新型提供一种新型的用于散射比浊法的光电测量装置，该装置是在一激光光源水平线前方，设置有透镜，透镜前方设置比色杯，杯内盛有液休，在比色杯前方设置吸收阱；在偏移吸收阱位置β角度处，设置滤色片，滤色片水平前方设置透镜，透镜前方设置光敏元件，光电二极管与电路板连接。所述偏移吸收阱位置β角度是15-45度。本实用新型的优点是，信号的信噪比高，抗干拢性能强，光路设计的尺寸小，结构简单。

摘要： 本实用新型涉及一种多光谱透射、散射比浊法和化学发光反射法检测用检测头及检测浊度仪，包括检测头本体，该检测头中部从顶端沿轴向制有一检测位盲孔和通孔；所述通孔分别设置可见光光源、紫外光光源和红外光光源及其直线相对应光电转换接收器；所述检测位盲孔的底部中心设有底部通孔和可见光源，检测头本体上制有上通孔，上通孔的内端安装有接收部件。本检测头设计结构精巧紧凑，检测速度快捷、成本低廉，集多种检测功能于一身：在可见光、紫外光和红外光三组波长光谱的范围内可分别对标本进行透射、散射多个项目或同一项目不同组分的检测；又可利用标本在光作用下的化学发光特性，进行反射法发色底物的检测，功能齐全，能够满足使用者的使用。

摘要： 一种利用激光散射透射比浊法进行蛋白质检测用测量杯，杯体的上口为圆柱形，中部“收腰”，下半部为圆柱形；杯体的内底部均布多个搅拌耳翼，杯体外部有两个搅拌柱销。本测量杯的结构简单、使用方便，使用该测量杯可提高检测效率、提高检测精度，节约检测成本。