摘要：
本文合成了钌络合物接枝的聚丙烯酸(Ru-PAA),以此作为电化学发光(ECL)聚合物.当钌络合物为PAA的16.2％(质量比)时,ECL发光聚合物中钌络合物的接枝量达到了饱和.利用Ru-PAA上残余的多羧基和被掺杂PAA的多羧基,以氮丙啶为羧基交联剂,交联制备了PAA掺杂的ECL纳米粒.在ECL纳米粒内掺杂PAA时,ECL强度会增加,表明非发光的聚电解质PAA能够增强纳米粒内部接枝的ECL组分的电致化学发光.当非发光的PAA掺杂量为1/20时,ECL信号增强了43.2％.同时,制备的纳米粒也表现出了一些良好的性质,包括在长达15天的考察期中粒径和粒径分布的结构稳定性、约20nm的小粒径和良好的水溶性.当然,表面的残余羧基也为生物标记与分析提供了良好的条件.%In this paper,ruthenium complex grafted polyacrylic acid(Ru-PAA),used as an electrochemiluminescent(ECL)polymer, had been synthesized. When ruthenium complex was 16. 2％(w/ w)of PAA,the grafting amount of ruthenium complex reached a saturation capacity. Based on the multiple carboxyl groups of PAA and the remaining ones of Ru-PAA,the ECL nanoparticles were prepared by carboxyl cross-linking polymerization of Ru-PAA and the doped PAA in the help of the cross-linker aziridine. When the ECL nanoparticles were doped by PAA,their ECL intensity increased. This indicated that the non-luminescent polyelectrolyte PAA could enhance the luminescent intensity of ECL components grafted inside the nanoparticle. When the doping amount of non-lumi-nescent PAA was 1 / 20,the ECL intensity was increased by 43. 2％. At the same time,the prepared nanoparticles also showed some good properties,including the structural stability of the particle size and its distribution,the signal stability in at least 15 days,the smaller particle size of about 20 nm,and the good water solubility. Certainly,the residual surface carboxyl groups also provided a good condition for bio-labeling and bio-analysis.
