摘要：
为研究圈养普氏野马Equus przewalskii对夏季高温刺激的生理应答,采用非损伤性取样法和放射性免疫法,随机选取5个圈养家族群中的成年雄马、成年雌马和成年带驹雌马各5匹,对其夏季高温期粪便内糖皮质激素和三碘甲腺原氨酸(T3)代谢产物的水平进行了测定.结果表明:(1)普氏野马在夏季高温期间一直维持在较高的激素水平;(2)不同类群的糖皮质激素代谢产物水平之间的差异有统计学意义(R2.60=22.885,P＜0.001),成年雄马＞成年雌马＞成年带驹雌马;(3)不同类群的T3代谢产物水平之间的差异有统计学意义(F2.60=37.780,P＜0.00l),成年雌马＞成年雄马＞成年带驹雌马;(4)3组组内不同应激程度(THI＞ 28.9 vs.THI≤28.9)下的激素水平差异无统计学意义,且组别与应激程度不存在交互作用.鉴于圈养普氏野马在夏季高温期不同类群的生理应答水平不同,建议对野马分组、分圈进行管护,防止高温造成意外损失;同时,建议利用非损伤性取样法对罔养普氏野马生理状况进行监测,为管理者进行有效的生存管理提供基础资料.%Physiological responses of captive Przewalski's horses (Equus przewalskii) in high temperature period of summer were investigated at Xinjiang Wild Horse Breeding and Research Center using radioimmunoassay by the non-invasive sampling method.We randomly selected 5 adult male horses,5 adult female horses and 5 lactating female horses from 5 family groups,to measure the levels of fecal metabolites of glucocorticoid and thyroid triiodothyronine (T3).The results showed that (1) E.przewalskii maintained high level of hormones in summer;(2) glucocorticoid metabolites level in 3 groups were significant different (F2.60 =22.885,P ＜ 0.001),and the order was male ＞ female ＞ lactating female;(3) Similarly,T3 level in different groups were also significantly different (F2.60 =37.780,P ＜ 0.001),and the order was female ＞ male ＞ lactating female;(4) However,the hormones performed no significant difference in various levels of heat stress (THI ＞28.9 vs.THI≤28.9).Meanwhile,there was no significant interaction between group and stress level.Overall,the captive E.przewalskii performed different physiological responses to high temperature stimulation in summer.In light of these findings,we propose that these horses should be fed separately to prevent accidental injury caused by high temperature in summer of Xinjiang.Moreover,the non-invasive measurement of fecal glucocorticoid and T3 can be introduced as a monitoring method for captive E.przewalskii during high temperature period of summer,and provide a scientific basis for artificial management.
