摘要：
目的 确定8种抗真菌药物对100株浅表致病真菌的体外药物敏感性.方法 参考美国临床和实验室标准化研究所(Clinical and Laboratory Standards Institute,CLSI)微量液基稀释法M27-A3和M38-A2方案,采用8种抗真菌药物对100株常见浅表致病真菌进行体外药物敏感性测定.结果 体外药敏实验结果显示,8种药物对皮肤癣菌表现出良好的体外药物敏感性,其中特比萘芬(GM MIC,0.011 μg/mL,MIC范围,0.004～0.06 μg/mL)具有最强的抗真菌活性.咪康唑(GM MIC,0.147 μg/mL,MIC范围0.015～1μg/mL)具有最强的抗念珠菌活性.酮康唑(GM MIC,0.097 μg/mL,MIC范围0.03～1 μg/mL)则对马拉色菌具有最佳体外抗真菌效果.结论 体外药敏实验结果表明常见浅表致病真菌对8种常用抗真菌药物表现出不同的敏感性.酮康唑具有广谱高效的特点,对临床常见皮肤癣菌、念珠菌、马拉色菌均具有良好的体外抗真菌活性.特比萘芬和萘替芬对皮肤癣菌具有最佳的抗真菌活性.%Objective To investigate the in vitro activity of 8 antifungal agents against 100 strains of pathogenic fungi isolated from patitens with superficial mycoses.Methods The antifungal susceptibility testing was investigated following Clinical and Laboratory Standards Institute (CLSI) reference broth microdilution methods.Eight antifungal agents were evaluated against 45 dermatophyte strains,35 Candida strains and 15 Malassezia strains.Results Our minimum inhibitory concentration (MIC) results revealed that terbinafine (GM MIC,0.011 μg/mL;MIC range,0.004-0.06 μg/mL) had the strongest antifungal activity against Dermatophyte,miconazole (GM MIC,0.147 μtg/mL;MIC range,0.015-1 μg/mL) against Candida,and ketoconazole (GM MIC,0.097 μg/mL;MIC range,0.03-1 μg/mL) against Malassezia.Conclusions The in vitro susceptibility testing indicates that common superficial pathogenic fungi exhibit variable susceptibilities to 8 antifungal agents.Ketoconazole has a potent antifungal activity for dermatophyte,Candida and Malassezia.Terbinafine and naftifine were the most active agents for Dermatophyte.
