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抗原递呈细胞

抗原递呈细胞的相关文献在1992年到2022年内共计150篇,主要集中在基础医学、肿瘤学、内科学 等领域,其中期刊论文120篇、会议论文4篇、专利文献117241篇;相关期刊87种,包括国际免疫学杂志、中国免疫学杂志、国际输血及血液学杂志等; 相关会议4种,包括中国畜牧兽医学会动物解剖及组织胚胎学分会第十八次学术研讨会、2008年首届国际中西医肿瘤研究论坛、中国美容与整形医师大会暨2005年度专科工作会议等;抗原递呈细胞的相关文献由423位作者贡献,包括林鑫、蒋俊、张学光等。

抗原递呈细胞—发文量

期刊论文>

论文:120 占比:0.10%

会议论文>

论文:4 占比:0.00%

专利文献>

论文:117241 占比:99.89%

总计:117365篇

抗原递呈细胞—发文趋势图

抗原递呈细胞

-研究学者

  • 林鑫
  • 蒋俊
  • 张学光
  • 王帆
  • 万兵
  • 侯宗柳
  • 俞英豪
  • 刘师节
  • 吴忠福
  • 周子珊
  • 期刊论文
  • 会议论文
  • 专利文献

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    • 程红燕; 向阳
    • 摘要: 虽然作用于程序性细胞死亡蛋白1/程序性死亡配体1(PD-1/PD-L1)和细胞毒性T淋巴细胞抗原4(CT-LA-4)的抗体已成功应用于晚期实体瘤的治疗,但其疗效仍不够高.需寻找新的免疫靶点以便为难治性肿瘤患者寻求替代治疗.根据受体与配体结合后发挥的作用,可将免疫靶点分为共刺激分子和共抑制分子,共抑制分子包括:T细胞免疫球蛋白黏蛋白-3(TIM-3)、含免疫球蛋白及ITIM结构域的T细胞免疫受体(TIGIT)、淋巴细胞活化基因3(LAG-3)、T细胞激活抑制物免疫球蛋白可变区结构域(VISTA)以及B7家族的B7-H3和B7-H4;共刺激分子包括CD27、OX40、4-1BB、CD40,糖皮质激素诱导的肿瘤坏死因子受体(GITR)和诱导共刺激因子(ICOS)等.本文就新兴的免疫靶点在妇科恶性肿瘤的临床前和临床研究进展作一简要阐述.
    • 梁悠燕
    • 摘要: 免疫调节是新课标高中生物的重要内容,教材章节的编排突出了免疫系统对机体稳态维持的作用,因此在教学过程中需要引导学生在生命活动调节中认识免疫,并且要关注免疫调节和神经调节、体液调节的关系,从整体上认识生命活动的调节。免疫与人类健康息息相关,但是学生缺乏相关知识,而且由于篇幅限制,教材内容较为简单,跳跃性强,学生难以理解。
    • Yonejima; 任天棋; 杨倩倩; 牛俊奇
    • 摘要: 【据《Hepatology》2019年4月报道】题:HBV感染者树突状细胞功能受损的特点(作者Yonejima A等)。树突状细胞是抗原递呈细胞,在宿主免疫反应中发挥核心作用。来自日本金泽大学的Yonejima等分析了患者因HBV感染而受损的树突状细胞功能,并发现了与其相关的基因。对64例HBV感染者和19例健康对照者的外周血单个核细胞进行分析。将外周血树突状细胞用HLA-DR/LIN-1/CD123/CD11C抗体染色,并通过荧光活化技术将细胞分为浆细胞样树突状细胞和髓样树突状细胞。采用IFNγ酶联免疫斑点法分析HBV感染者的抗原特异性反应。此外,还具体分析树突状细胞的抗原递呈能力、细胞迁移能力、吞噬能力和细胞因子产生能力的变化。通过微阵列分析树突状细胞基因表达以识别与树突状细胞功能相关的基因。
    • 刘壹菲; 薛晓旭; 李正宜; 王军朋; 张祎捷
    • 摘要: This study was designed to explore the effect of apigenin (Api) on dendritic cell (DCs) maturation and function in murine spleen cells.The single spleen cell was isolated,and then cultured with lipopolysaccharide (LPS) in the present and absence of apigenin.After 24 h,the toxicity of Api and the T cell proliferation were determined by CCK8 kit.In addition,we collected the cell-free supernatants to measure cytokine production using ELISA,collected the cells to determine the DC maturation using flow cytometry.Finally,we purified Api and/or LPS-treated CD11c+ DCs which were pulsed with ovalbumin (OVA)323-339 and then were adoptive transferred into C57BL/6 mice to detect the OVA323-339-specific T cell proliferation and T helper (Thl) and Th2 cell secreting IFN-γ and IL-4 production,respectively.We found that Api did not affect splenocyte viability,but inhibited the production of pro-inflammatory cytokine IL-1β,IL-6 and TNF-α,not anti-inflammatory cytokine IL-10.In addition,Api inhibited the expression of co-stimulatory CD80,CD86 and MHCII of CD11c + DCs.Finally,compared to LPS+OVA DCs group,DCs from Api and LPS co-treated splenocytes (Api+LPS+DCs) impaired OVA323-339-specific T cell proliferation and the production of IFN-y and IL-4 in CD4+ T cells,which had the similar responses with OVA+DCs.These data suggest that Api exhibits anti-inflammatory properties via inhibiting DC activation and function,as a new immune-modulator,which may induce immune-tolerance with a benefit to those with chronic inflammation.%探讨芹菜素对小鼠中抗原递呈细胞特别是树突状细胞(dendritic cells,DCs)成熟和递呈抗原能力的影响.体外分离成年小鼠脾细胞后,经脂多糖(lipopolysaccharide,LPS)和芹菜素共培养24 h后,Cell Counting Kit (CCK)-8、酶联免疫吸附实验(enzyme-linked immunosorbent assay,ELISA)和流式细胞术等方法分别检测芹菜素对脾细胞的毒性作用、分泌炎症细胞因子的作用、共刺激分子表达的影响;分选芹菜素和/或LPS处理后的DCs,与卵清白蛋白(ovalbumin,OVA) 323-339共孵育并过继转移到正常小鼠体内7天后,分离T细胞并经OVA323-339重新刺激后,CCK-8法和流式细胞仪检测OVA323 339特异性T细胞的增殖和分泌炎症细胞因子的能力.结果显示,芹菜素抑制LPS刺激的脾细胞分泌促炎症细胞因子白细胞介素(interleukin,IL)-1β、IL-6和肿瘤坏死因子(tumor necrosis factor,TNF)-a的产生,但不影响抗炎症细胞因子IL-10的产生,而该效应并非依赖其毒性杀伤作用.进一步研究发现,芹菜素抑制脾细胞CD11c+ DCs的共刺激分子CD80、CD86和MHCII的表达,提示芹菜素可能诱导DCs的不成熟或减弱其抗原递呈能力.体内功能实验结果显示,芹菜素处理的DCs刺激抗原特异性T细胞的增殖及分泌细胞因子[辅助性T细胞(Th)1:IFN-γ和Th2:IL-4]的能力显著减弱.综上所述,芹菜素的抗炎作用可能是通过调控脾细胞中DCs的成熟和递呈抗原的能力,从而在一定程度上抑制炎症细胞因子的产生.
    • 吴植; 郝福星; 王永娟; 刘洋; 王栋; 徐州; 袁维峰
    • 摘要: To design chimeric DNA vaccine targeted to antigen-presenting cells with enhanced effi-cacy to induce immunization.The plasmid containing the gene encoding the extracelluar domain of canine cytotoxic T-lymphocyte antigen 4(CTLA-4 1 2 5 ),was directly fused to the gene fragment for major antigenic epitopes of VP2(VP2 228 )by molecular engineering technology.The plasmid con-taining VP2 228 alone was also constructed to serve as control and transfection of COS-7 cells with the two resultant plasimds was performed respectively,followed by assay of Western blotting. The mice were immunized with the constructed two plasimds,respectively.After immunization, the antibodies anginst CPV in the immunized mice at different times were measured by HI.The spleen lymphocyte proliferation response was determined by lymphocyte proliferation assay,and the interferon-γ(IFN-γ)expression level of the mouse lymphocytes were measured by ELISA. The eukaryotic expression plasimds of CTLA-4 1 2 5-VP2 228 fusion protein or VP2 228 alone were cloned.Western blotting showed that the two recombinant proteins could be expressed.Immuni-zation results showed that the antibody levels in serum of CTLA-4 1 2 5-VP2 228-immunized mice were significantly higher than that of VP2 228-immunized mice (P <0.05).The lymphocyte stimulation indexes and secreted IFN-γ levels of the CTLA-4 1 2 5-VP2 228-immunized mice were significantly higher than that of VP2 228-immunized mice (P < 0.05 and P < 0.01 ),respectively.CTLA-4 1 2 5-VP2 228 chimeric DNA vaccine stimulates strong immune response in mice,making it possible for further exploration into chimeric DNA that target the antigen to APCs.%研究旨在构建特异性靶向犬细小病毒(canine parvovirus,CPV)融合 DNA 疫苗,探讨其诱导机体产生免疫应答的效果。试验用重组技术构建了含细胞毒性 T 淋巴细胞抗原-4胞外区(CTLA-4125)与犬细小病毒 VP2的主要抗原表位区域(VP2228)融合表达质粒 pVAX1-CTLA-4125-VP2228,同时构建了不含 CTLA-4125的 pVAX1-VP2228,体外转染 COS-7细胞,Western blotting 检测其表达产物;将 pVAX1-CTLA-4125-VP2228、pVAX1-VP2228分别免疫小鼠,免疫后进行抗体水平测定和抗体亚型分析;通过淋巴细胞增殖试验和 ELISA 分别检测淋巴细胞刺激指数和γ-干扰素表达水平。结果显示成功构建了 pVAX1-CTLA-4125-VP2228和 pVAX1-VP2228,并能在 COS-7细胞中正确表达;抗体检测结果显示 pVAX1-CTLA-4125-VP2228免疫组抗体水平显著高于 pVAX1-VP2228免疫组(P <0.05);淋巴细胞增殖试验显示 pVAX1-CTLA-4125-VP2228免疫组的刺激指数显著高于 pVAX1-VP2228免疫组(P <0.05);γ-干扰素表达水平测定显示 pVAX1-CTLA-4125-VP2228免疫组极显著高于 pVAX1-VP2228免疫组(P <0.01)。结果表明,CTLA-4125-VP2228融合 DNA 能有效增强动物对 VP2228抗原的免疫应答,为进一步研究融合 DNA 疫苗特异性的靶向递呈机制奠定基础。
    • 白伶伶; 张灵君; 郑慧; 王梅艳; 东莉洁; 李筱荣; 张晓敏
    • 摘要: Background Our previous studies found that mesenchymal stem cells (MSCs) can ameliorate experimental autoimmune uveitis (EAU) and reduce tissue impairment.Its mechanism is still pending.Objective This study was performed to investigate the effects of MSCs on T cell subsets and antigen presenting cells (APCs) in EAU rats.Methods MSCs were isolated from bone marrow of six male Wistar rats and cultured by plastic adherence method.Twelve female Lewis rats were assigned randomly into MSCs group and PBS group.EAU rat model was induced by immunization with 200 μl emulsion containing 30 μg interphotoreceptor retinoid-binding protein (IRBP) 1177-1191 polypeptide fragment R16 and complete Freund adjuvant (CFA).The eye manifestations of the rats were observed and scored under the slit lamp microscope after modeling.The R16-immunized rats were treated intravenously with 5×106/ml MSCs for 3 consecutive days from day 9 to 11 after modeling in the MSCs group,and the equivalent volume of PBS was used with the same way in the PBS group.Fifteen days after modeling,the spleens and draining lymph nodes were collected to evaluate the proportion of interferon-γ (IFN-γ) positive CD4+ T cells,interleukin-17 (IL-17)positive CD4+ T cells and forkhead helix transcription factor p3 (Foxp3) positive CD4+ T cells by flow cytometry.The T cells and APCs from the different groups were cocultured and divided into PBS cocultured group,MSCs cocultured group, PBS-MSCs cross-cultured group and MSCs-PBS cross-cultured group under the stimulation of R16 at the concentration of 0.3,1.0 or 10.0 μg/ml, and the proliferation indexes of the T cells in different groups were assayed by 5-bromodeoxyuridine (BrdU) Elisa kit.The use of experimental animals complied with the regulations on the management of experimental animals promulgated by the national science and technology commission.Results The ocular surface inflammatory scores of 11,12,13 and 14 days after modeling in the MSCs group were significantly lower than that in the PBS group (t=3.825,5.100,4.250,3.400, all at P<0.05).Compared with the PBS group, the proportions of IFN-γ positive CD4+ T cells in spleen and draining lymph notes were considerably decreased in the MSCs group (t =5.651,4.376, both at P<0.05) , so were the IL-17+ CD4+ T cells (t =3.300,4.925, both at P<0.05).However,the proportions of Foxp3 + CD4+ T cells in spleen and draining lymph notes were statistically raised in the MSCs group compared with the PBS group (t =-5.172,-2.825,both at P<0.05).The proliferation index of T cells increased with the rise of R16 dose in the PBS cocultured group, and the proliferation indexes were all declined in the MSCs cocultured group compared with the PBS cocultured group under the stimulation of 0.3,1.0 and 10.0 μg/ml of R16 (P =0.027,0.000,0.000).In addition, significant reduces of proliferation indexes of T cells were seen in the PBS-MSCs cross-cultured group and MSCs-PBS cross-cultured group in comparison with the PBS cocultured group when stimulated by 1.0 μg/ml and 10.0 μg/ml R16 (1.0 μg/ml R16 : P =0.001,0.000;10.0 μg/ml R16:P=0.000,0.000).Conclusions MSCs can ameliorate EAU by inhibiting the functions of antigen-specific T cells and APCs and up-regulating T regulatory cells in EAU rats.%背景 我们前期研究发现,间充质干细胞(MSCs)可以有效治疗大鼠实验性自身免疫性葡萄膜炎(EAU),减轻组织损害,但其具体作用机制仍在研究中.目的 研究MSCs对大鼠EAU模型中T细胞亚群和抗原递呈细胞(APCs)的影响.方法 收集6只清洁级4~6周龄Wistar雄性大鼠双侧股骨、胫骨骨髓,采用贴壁培养法纯化Wistar大鼠骨髓MSCs.采用随机数字表法将12只清洁级Lewis雌性大鼠分为MSCs组和PBS组,每组6只.于Lewis大鼠单后足及背部皮下注射200μl含30μg光感受器间维生素A类结合蛋白(IRBP) 1177-1191多肽片段R16及完全弗氏佐剂(CFA)的乳化液以建立EAU模型,造模后于裂隙灯显微镜下观察大鼠眼部炎症表现.造模后9~11d,MSCs组大鼠每日经尾静脉注射密度为5×106/ml的MSCs悬液1 ml;PBS组大鼠以同样的方法注射等容积的PBS.造模后15d,分离各组大鼠脾脏和引流淋巴结中的T细胞及APCs,采用流式细胞仪检测各组大鼠脾脏和引流淋巴结中γ干扰素(IFN-γ)阳性CD4+T细胞、白细胞介素-17(IL-17)阳性CD4+T细胞和叉头状螺旋转录因子p3(Foxp3)阳性CD4+T细胞的比例,以评估辅助性T细胞1(Th1)、Th17和调节性T细胞(Treg)细胞亚群的作用;依据各组T细胞与APCs共培养的方式不同分为PBS共培养组、PBS-MSCs交叉培养组、MSCs-PBS培养组和MSCs共培养组,分别加入不同质量浓度(0.3、1.0、10.0 μg/ml)的R16抗原进行刺激,无R16抗原刺激的细胞作为空白对照,采用5-溴脱氧尿嘧啶核苷(BrdU)法测定各组大鼠T细胞吸光度(A)值,计算T细胞增生指数.结果 造模后11、12、13和14d,MSCs组大鼠眼前节炎症评分均明显低于PBS组,差异均有统计学意义(t=3.825、5.100、4.250、3.400,均P<0.05).与PBS组大鼠比较,MSCs组大鼠脾脏和淋巴结中IFN-γ+ CD4+T细胞比例均明显下降,差异均有统计学意义(t=5.651、4.376,均P<0.05);MSCs组大鼠脾脏和引流淋巴结中IL-17+CD4+T细胞比例均明显下降,差异均有统计学意义(t=3.300、4.925,均P<0.05),Foxp3+ CD4+T细胞的比例均明显升高,差异均有统计学意义(t=-5.172、-2.825,均P<0.05).PBS共培养组大鼠脾脏T细胞增生指数随着R16抗原质量浓度的增加而升高,在各自质量浓度(0.3、1.0和10.0 μg/ml)R16刺激条件下,MSCs共培养组T细胞增生指数较PBS共培养组明显下降,差异均有统计学意义(P=0.027、0.000、0.000);在R16质量浓度为1.0 μg/ml和10.0 μg/ml条件下,MSCs-PBS交叉培养组和PBS-MSCs交叉培养组T细胞增生指数均明显低于PBS共培养组,差异均有统计学意义(1.0μg/ml R16:P=0.001、0.000;10.0μg/ml R16:P=0.000、0.000).结论 MSCs可通过同时抑制EAU大鼠体内抗原特异性T细胞和APCs的功能以及上调Treg细胞比例来发挥对大鼠EAU的治疗作用.
    • 白伶伶12; 张灵君1; 郑慧1; 王梅艳1; 东莉洁1; 李筱荣1; 张晓敏1
    • 摘要: 背景 我们前期研究发现,间充质干细胞(MSCs)可以有效治疗大鼠实验性自身免疫性葡萄膜炎(EAU),减轻组织损害,但其具体作用机制仍在研究中.目的 研究MSCs对大鼠EAU模型中T细胞亚群和抗原递呈细胞(APCs)的影响.方法 收集6只清洁级4~6周龄Wistar雄性大鼠双侧股骨、胫骨骨髓,采用贴壁培养法纯化Wistar大鼠骨髓MSCs.采用随机数字表法将12只清洁级Lewis雌性大鼠分为MSCs组和PBS组,每组6只.于Lewis大鼠单后足及背部皮下注射200μl含30μg光感受器间维生素A类结合蛋白(IRBP) 1177-1191多肽片段R16及完全弗氏佐剂(CFA)的乳化液以建立EAU模型,造模后于裂隙灯显微镜下观察大鼠眼部炎症表现.造模后9~11d,MSCs组大鼠每日经尾静脉注射密度为5×106/ml的MSCs悬液1 ml;PBS组大鼠以同样的方法注射等容积的PBS.造模后15d,分离各组大鼠脾脏和引流淋巴结中的T细胞及APCs,采用流式细胞仪检测各组大鼠脾脏和引流淋巴结中γ干扰素(IFN-γ)阳性CD4+T细胞、白细胞介素-17(IL-17)阳性CD4+T细胞和叉头状螺旋转录因子p3(Foxp3)阳性CD4+T细胞的比例,以评估辅助性T细胞1(Th1)、Th17和调节性T细胞(Treg)细胞亚群的作用;依据各组T细胞与APCs共培养的方式不同分为PBS共培养组、PBS-MSCs交叉培养组、MSCs-PBS培养组和MSCs共培养组,分别加入不同质量浓度(0.3、1.0、10.0 μg/ml)的R16抗原进行刺激,无R16抗原刺激的细胞作为空白对照,采用5-溴脱氧尿嘧啶核苷(BrdU)法测定各组大鼠T细胞吸光度(A)值,计算T细胞增生指数.结果 造模后11、12、13和14d,MSCs组大鼠眼前节炎症评分均明显低于PBS组,差异均有统计学意义(t=3.825、5.100、4.250、3.400,均P<0.05).与PBS组大鼠比较,MSCs组大鼠脾脏和淋巴结中IFN-γ+ CD4+T细胞比例均明显下降,差异均有统计学意义(t=5.651、4.376,均P<0.05);MSCs组大鼠脾脏和引流淋巴结中IL-17+CD4+T细胞比例均明显下降,差异均有统计学意义(t=3.300、4.925,均P<0.05),Foxp3+ CD4+T细胞的比例均明显升高,差异均有统计学意义(t=-5.172、-2.825,均P<0.05).PBS共培养组大鼠脾脏T细胞增生指数随着R16抗原质量浓度的增加而升高,在各自质量浓度(0.3、1.0和10.0 μg/ml)R16刺激条件下,MSCs共培养组T细胞增生指数较PBS共培养组明显下降,差异均有统计学意义(P=0.027、0.000、0.000);在R16质量浓度为1.0 μg/ml和10.0 μg/ml条件下,MSCs-PBS交叉培养组和PBS-MSCs交叉培养组T细胞增生指数均明显低于PBS共培养组,差异均有统计学意义(1.0μg/ml R16:P=0.001、0.000;10.0μg/ml R16:P=0.000、0.000).结论 MSCs可通过同时抑制EAU大鼠体内抗原特异性T细胞和APCs的功能以及上调Treg细胞比例来发挥对大鼠EAU的治疗作用.
    • 张洋林; 刘燕
    • 摘要: 人教版高中生物教材有关特异性免疫反应过程中有很多疑点,教师如果能适当对这些疑点进行解释和完善,就可以有效帮助学生突破相关重难点知识。l特异性免疫过程图解涉及的抗原为蛋白质类抗原吗?根据产生抗体时是否需要T细胞的参与可将抗原分为胸腺依赖性抗原(TD抗原)和胸腺非依赖性抗原(TI抗原)两类。
    • 于雄伟; 薄禄龙; 邓小明
    • 摘要: 背景 T细胞免疫球蛋白及黏蛋白域蛋白(T cell immunoglobulin domain and mucin domain,TIM)家族能调节T细胞免疫反应.TIM4只表达在抗原递呈细胞表面.作为T细胞活化强有力的免疫共刺激分子,调控Th细胞的分化增殖. 目的 研究TIM4与其配体结合后调节T细胞免疫反应的机制,探究TIM4在疾病中的作用. 内容 TIM4的基础研究进展,TIM4在疾病中的研究进展. 趋向TIM4对T细胞增殖的促进或抑制作用取决于其结合的配体和T细胞分化的程度.TIM4的表达异常与多种过敏性或自身免疫性疾病的发生有关,可能成为药物治疗的新靶点.
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