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心肌线粒体

心肌线粒体的相关文献在1987年到2022年内共计148篇,主要集中在体育、内科学、基础医学 等领域,其中期刊论文135篇、会议论文11篇、专利文献6045篇;相关期刊92种,包括体育科技文献通报、体育科学、中国体育科技等; 相关会议10种,包括首届岭南内科大会、全国第四次麻醉药理学学术会议暨2013年贵州省麻醉学术年会、2013年中医药防治心病学术年会等;心肌线粒体的相关文献由364位作者贡献,包括单虎、张明、潘晓青等。

心肌线粒体—发文量

期刊论文>

论文:135 占比:2.18%

会议论文>

论文:11 占比:0.18%

专利文献>

论文:6045 占比:97.64%

总计:6191篇

心肌线粒体—发文趋势图

心肌线粒体

-研究学者

  • 单虎
  • 张明
  • 潘晓青
  • 闫蕊
  • 魏瑾
  • 刘铁民
  • 林琳
  • 王欢
  • 胡元会
  • 丁树哲
  • 期刊论文
  • 会议论文
  • 专利文献

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    • 李琪琳; 胡元会; 王欢; 王强; 张林淮
    • 摘要: 心肌线粒体损伤是心力衰竭病理生理机制的重要环节之一,线粒体保护途径已成为防治心力衰竭损伤的研究热点.中医药能够通过稳定线粒体膜电位、增强呼吸链复合体活性、调节钙浓度、减轻氧化应激、调控细胞凋亡、保护mtDNA等作用来减轻心力衰竭对心肌线粒体的损伤.本文就近年来中医药改善心力衰竭心肌线粒损伤研究进展作一综述,以期为中医药防治心衰提供客观的科学依据.
    • 潘文文; 吴立明; 崔洁
    • 摘要: 为了探讨山桐子油对力竭运动大鼠心肌线粒体的保护作用,将50只SD大鼠随机分成对照组,力竭运动组,低、中、高剂量山桐子油组,其中对照组不进行任何运动,力竭运动组进行跑步运动训练,低、中、高剂量山桐子油组进行跑步运动的同时在运动之后2h分别灌胃山桐子油,实验持续6周,每周6日,周日休息.研究结果表明:力竭运动组大鼠心肌线粒体内的ATP合成能力、SOD活性、GSH-Px活性、CAT活性与对照组相比均明显降低(P< 0.05,P<0.01),Ca2+含量、MDA含量与对照组相比明显增加(P<0.05,P<0.01);低、中、高剂量山桐子油组大鼠的ATP合成能力、SOD活性、GSH-Px活性、CAT活性呈上升趋势,Ca2+含量、MDA含量呈下降趋势;高剂量山桐子油组大鼠的ATP合成能力、SOD活性、GSH-Px活性、CAT活性、Ca2+含量、MDA含量与力竭运动组相比具有显著性差异(P <0.05,P<0.01).说明山桐子油对力竭运动大鼠心肌线粒体有很好的保护作用.
    • 徐席斌; 刘晨涛
    • 摘要: To explore the effect and underlying mechanisms of resistance training on cardiac function and structure and function of myocardial mitochondria in aged rats.Establishing resistance training model of aged rats,the rats were divided into three groups:young control group (y-Ctrl),elderly control group (e-Ctrl) and elderly resistance training group (e-RT).We detected heart coefficient and haemodynamics parameters of rats,and observed myocardial morphological change and myocardial mitochondrial structure change of rats.The expressions of PGC1α,SIRT1,AMPKα1,AMPKα2 were measured in myocardium of rats.Compared with eCtrl,the heart coefficient was increased significantly in rats of e-RT,while haemodynamics parameters,including left ventricular systolic pressure,left ventricular end-diastolic pressure,the maximal ascending rate of left ventricular pressure,and the maximal descending rate of left ventricular pressure,all were reduced dramatically in rats of e-RT.Resistance training enhanced number of aged cardiocytes to make its arrangement and distribution more closely,and alleviated content of collagen fiber in myocardium of aged rats.Compared with eCtrl,the expressions of PGC1α,SIRT1,AMPKα1,AMPKα2 were improved remarkably in myocardium of rats of e-RT.Long-term resistance training can improve aged cardiac function,and reduce systolic and diastolic pressure of aged rats.In addition,it can protect structural integrality of myocardium and myocardial mitochondria,the underlying mechanisms are associated with AMPK/PGC1α/SIRT1 signaling pathway to improve myocardial mitochondrial function of aged rats.Resistance training can be regarded as an economical and safe method for preventing and delaying senescence.%探讨抗阻力运动对衰老的大鼠心功能及心肌线粒体结构功能影响的机制.建立老年大鼠的抗阻力运动模型,实验分为3组:青年对照组(y-Ctrl),老年对照组(e-Ctrl)和老年抗阻力运动组(e-RT).检测大鼠心系数、血流动力学指标,观测大鼠心肌的形态学变化以及心肌线粒体结构的改变.检测大鼠心肌PGC1α,SIRT1,AMPKα1,AMPKα2的表达.与e-Ctrl组相比,e-RT组大鼠的心系数显著提高,而左室收缩压、左室舒张末压、左室压力最大上升速率和最大下降速率等血流动力学指标都显著下降.抗阻力运动提高衰老的心肌细胞的数量,使心肌细胞排列和分布更加紧密,并且可以减少胶原纤维的含量.与e-Ctrl组相比,e-RT组大鼠心肌的PGC1α,SIRT1,AMPKα1,AMPKα2的表达显著增加.长期抗阻力运动可以改善衰老的心功能,降低老年大鼠的收缩压和舒张压,保护心肌以及心肌线粒体结构的完整性,可能通过AMPK/PGC1α/SIRT1信号通路改善老年大鼠的心肌线粒体功能.抗阻力运动可作为老年人预防和延缓衰老的一种经济安全的方法.
    • 李琪琳; 胡元会; 吴华芹; 王欢; 孙伟; 王辉奇
    • 摘要: 目的:研究心复康口服液对心力衰竭大鼠心肌线粒体蛋白质组学的影响,探讨其可能的分子作用机制.方法:SD大鼠随机分为造模组与假手术组,造模组采用结扎左冠状动脉前降支致心肌梗死后心力衰竭大鼠模型,术后随机分为模型组、心复康口服液组,各组连续灌胃给药8周后采集大鼠心肌,采用双向凝胶电泳和基质辅助激光解析电离飞行时间质谱技术,分析比较各组大鼠心肌线粒体蛋白质组学表达的差异,并运用蛋白免疫印迹技术对差异表达蛋白进行回复性验证.结果:模型组与假手术组相比有差异而心复康口服液可使差异趋势逆转的蛋白点为20个,其中13个表达上调,7个表达下调,经质谱分析成功鉴定出13个蛋白,主要涉及能量代谢、应激反应、氧化损伤、细胞骨架、细胞分化增殖等功能.Western blot结果显示模型组Stress-70、Nucleophosmin表达上调,ATP-α表达下调,心复康口服液组Stress-70、Nucleophosmin表达下调,ATP-α表达上调,与蛋白质组学结果一致.结论:心复康口服液可在一定程度上调节心衰大鼠心肌线粒体蛋白质组学变化,其干预机制可能与改善能量代谢、减轻应激反应、抗氧化损伤及调节细胞分化增殖等功能相关.采用双向凝胶电泳和基质辅助激光解析电离飞行时间质谱技术,以心肌线粒体为切入点,研究心复康口服液对心衰大鼠心肌线粒体蛋白质组学的影响,结果真实可靠.%This paper aimed at investigating the effects of Xinfukang Oral Liquid on mitochondrial proteomic alterations in rats with heart failure after myocardial infarction and exploring its possible mechanisms. Heart failure models were established by ligating the left anterior descending coronary artery of SD rats. Rats were randomly divided into sham group, model group, Xinfukang group. 8 weeks after drug intervention, the mitochondrial proteomic alterations of myocardial tissue were detected by two-dimensional gel electrophoresis (2-DE) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) . Furthermore, expression levels of part of the differently expressed proteins were verified by western blot. Compared with model group, 20 differentially expressed protein spots were detected in Xinfukang group, 13 of which showed increased protein expression and 7 decreased; 13 differentially expressed protein spots were successfully identified by MALDI-TOF-MS. Bioinformatics analysis showed that these differential proteins were mainly associated with energy metabolism, stress reaction, oxidative damage, cyto-skeleton, cell differentiation and proliferation. Western blot results showed that the expression of Stress-70 protein and Nucleophosmin increased and the expression of ATP-αdecreased in model group. The expression of Stress-70 protein and Nucleophosmin decreased and the expression of ATP-αincreased in Xinfukang group, which shows the same results in proteomics. Xinfukang Oral Liquid can partly adjust proteomic alterations of myocardial mitochondrial in HF rats, and its intervention mechanism may involve improving energy metabolism, reliving stress reaction and oxidative damage, as well as regulating cell differentiation and proliferation. The results of comparative proteomic analysis performed by using2-DE and MALDI-TOF-MS are acurate, stable and reliable.
    • 李琪琳; 胡元会; 霍艳明; 刘瑞华; 吴华芹; 王欢
    • 摘要: 目的:研究曲美他嗪对气虚血瘀证心衰大鼠心肌线粒体蛋白质组学的影响.方法:SD大鼠随机分为造模组与假手术组,造模组采用结扎左冠前降支致心梗后气虚血瘀证心衰大鼠模型,术后随机分为模型组、曲美他嗪组,各组连续灌胃给药8 周后采集大鼠心肌,采用双向凝胶电泳和基质辅助激光解析电离飞行时间质谱技术,比较各组大鼠心肌线粒体蛋白质组学差异,并运用蛋白免疫印迹技术对差异蛋白进行回复性验证.结果:模型组与假手术组相比有差异而曲美他嗪可使差异趋势逆转的蛋白点为18 个,经质谱分析成功鉴定出10个蛋白,主要涉及能量代谢、氧化损伤、应激反应、细胞骨架、细胞分化增殖等功能.Western blot 结果显示模型组Stress-70、Nucleophosmin 表达上调,ATP-α表达下调,曲美他嗪组Stress-70、Nucleophosmin 表达下调,ATP-α表达上调,与蛋白质组学结果一致.结论:曲美他嗪可一定程度调节模型大鼠心肌线粒体蛋白质组学变化,其干预机制可能与改善能量代谢、抗氧化损伤、减轻应激反应及调节细胞分化增殖等功能相关.采用双向凝胶电泳和质谱技术研究曲美他嗪对气虚血瘀证心衰大鼠心肌线粒体蛋白质组学的影响,结果真实可靠.%Objective: To investigate the effects of Trimetazidine on mitochondrial proteomic alterations in heart failure rats with qi- deficiency and blood- stasis syndrome after myocardial infarction. Methods: Heart failure models were established by ligating the left anterior descending coronary artery of SD rats. Rats were randomly divided into sham group, model group, Trimetazidine group. 8 weeks after drug intervention, the mitochondrial proteomic alterations of myocardial tissue were detected by two-dimensional gel electrophoresis (2-DE) and matrix-assisted laser desorption/ ionization time- of- flight mass spectrometry (MALDI- TOF- MS). Furthermore, expression levels of part of the differentially expressed proteins were verified by western blot. Results: Compared with model group, 18 differentially expressed protein spots were detected in Trimetazidine group, 10 of which were successfully identified by MALDI-TOFMS. Bioinformatics analysis showed that these differential proteins were mainly associated with energy metabolism, stress reaction, oxidative damage, cyto-skeleton, cell differentiation and proliferation. Western blot results showed that the expression of Stress-70 protein and Nucleophosmin increased and the expression of ATP-αdecreased in model group. The expression of Stress- 70 protein and Nucleophosmin decreased and the expression of ATP- αincreased in Trimetazidine group, which showed the same results in proteomics. Conclusion: Trimetazidine can partly adjust proteomic alterations of myocardial mitochondrial in HF rats with qi-deficiency and blood-stasis syndrome, and its intervention mechanism may involve improving energy metabolism, relieving stress reaction and oxidative damage, as well as regulating cell differentiation and proliferation. The results of comparative proteomic analysis performed by using 2-DE and MALDI-TOF-MS are accurate, stable and reliable.
    • 李琪琳; 胡元会; 霍艳明; 王辉奇; 王强; 王欢
    • 摘要: 目的 研究卡托普利对心衰大鼠心肌线粒体蛋白质组学的影响.方法 SD大鼠随机分为造模组与假手术组;造模组采用结扎冠脉致心梗后心衰模型,术后再随机分为模型组、卡托普利组;各组连续予以相应干预8周后采集大鼠心肌,采用双向凝胶电泳和质谱技术,分析各组大鼠心肌线粒体蛋白质组学变化,并运用Western blot技术对差异蛋白进行回复性验证.结果 模型组与假手术组相比有差异,且卡托普利能使差异趋势逆转的蛋白点为19个,其中11个表达上调,8个表达下调;经质谱分析成功鉴定出12个蛋白,主要涉及能量代谢、氧化损伤、应激反应、细胞骨架、细胞分化增殖等功能. Western blot结果显示模型组ATP-α表达下调,Stress-70、Nucleophosmin表达上调;卡托普利组ATP-α表达上调,Stress-70、Nucleophosmin表达下调,与蛋白质组学结果基本一致.结论 卡托普利可一定程度调节心衰大鼠心肌线粒体蛋白质组学变化,其干预机制可能与改善能量代谢、抗氧化损伤、减轻应激反应及调节细胞分化增殖等功能相关.
    • 魏义勇; 刘云; 喻守佳; 王海英; 喻田
    • 摘要: 目的 寻找心肌线粒体蛋白质组学研究中样本制备关键环节的可行性方案.方法 分为组织匀浆组(TH组)、差数离心提取心肌线粒体组(CM组)及差数离心后再通过Nycodenz密度梯度离心法提纯线粒体组(PM组).通过Western blot法检测各亚细胞器标志性蛋白的含量验证线粒体的纯度,提取CM组与PM组中的蛋白质行双向凝胶电泳(2-DE),观察硝酸银染色后CM组与PM组的凝胶图像,选取PM组中的部分蛋白质斑点酶解后质谱鉴定.结果 本实验提纯的线粒体纯度较高;PM组蛋白质2-DE后凝胶银染图像较CM组更清晰,背景更佳;选取的部分蛋白质经鉴定均为线粒体蛋白.结论 本实验为心肌线粒体蛋白质组学研究中的样本制备提供了较好的方法.
    • 谢一锋
    • 摘要: 力竭性运动使运动的机体耗氧过多,局部组织缺氧、代谢产物堆积,对线粒体的氧化功能产生影响.罗汉果叶片中的黄酮物质具有活血化瘀的药理作用,并有较强的抗氧化性,其抗氧化性优于化学制剂,能有效清除运动导致机体内产生的过量自由基.本研究拟构建力竭运动动物模型,通过离体实验测试来探索研究罗汉果叶黄酮对运动大鼠心肌线粒体的影响.
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