心肌存活性

摘要： 目的冠脉CT血管造影(CT angiography,CTA)对于评估急性ST段抬高型心肌梗死(ST-segment elevated myocardial infarction,STEMI)后心肌存活性的诊断效力进行分析。方法该研究选取兰州市第一人民医院心内科2016年1月—2018年1月就诊的STEMI患者201例纳入研究,所有患者均接受CTA和核素心肌显像(emission computed tomography,ECT)检查,并分别评估心肌存活性情况,并针对冠脉CTA的评估效力进行分析。结果冠脉CTA检查显示存活心肌131例,失活心肌70例,ECT显示存活心肌138例,失活心肌63例。两种方法筛查诊断出的存活与失活心肌组患者的差异无统计学意义(χ^(2)=2.769,P=0.092)。心肌存活组与心肌失活组患者的年龄和左室射血分数差异有统计学意义(P0.05)。冠脉CTA评估心肌失活的灵敏度为92.75%(128/138),假阴性概率(漏诊率)为7.25%(10/138),特异度为95.24%(60/63),假阳性概率为4.76%(3/63)。ROC曲线的曲线下面积为0.847,约登指数为0.88。结论冠脉CTA评估STEMI后心肌存活性具有优良的灵敏度和特异度,可供临床参考使用。

摘要： 目的 冠脉CT血管造影(CT angiography,CTA)对于评估急性ST段抬高型心肌梗死(ST-segment elevated myocardial infarction,STEMI)后心肌存活性的诊断效力进行分析.方法 该研究选取兰州市第一人民医院心内科2016年1月—2018年1月就诊的STEMI患者201例纳入研究,所有患者均接受CTA和核素心肌显像(emission computed tomography,ECT)检查,并分别评估心肌存活性情况,并针对冠脉CTA的评估效力进行分析.结果 冠脉CTA检查显示存活心肌131例,失活心肌70例,ECT显示存活心肌138例,失活心肌63例.两种方法筛查诊断出的存活与失活心肌组患者的差异无统计学意义(χ2=2.769,P=0.092).心肌存活组与心肌失活组患者的年龄和左室射血分数差异有统计学意义(P0.05).冠脉CTA评估心肌失活的灵敏度为92.75％(128/138),假阴性概率(漏诊率)为7.25％(10/138),特异度为95.24％(60/63),假阳性概率为4.76％(3/63).ROC曲线的曲线下面积为0.847,约登指数为0.88.结论 冠脉CTA评估STEMI后心肌存活性具有优良的灵敏度和特异度,可供临床参考使用.

摘要： 目的:观察早期应用重组人脑利钠肽对前壁AMI急诊PCI术后心肌存活性及心功能的影响.方法:我院2018年1月-2019年1月收治的60例前壁AMI急诊PCI术患者为本次研究对象,按照早期是否应用重组人脑利钠肽将所有患者分为对照组(30例:为应用重组人脑利钠肽)与实验组(30例:应用重组人脑利钠肽),比较两组患者预后.结果:实验组患者术后节段改善率明显高于对照组,P<0.05.实验组患者术后1个月左室射血分数(LVEF)、左室舒张末期容量指数(LVEDV)以及左室收缩末期容量指数(LVESV)等相关心功能数值均优于对照组,P<0.05.结论:前壁AMI急诊PCI术患者早期应用重组人脑利钠肽治疗效果明显优于未早期应用重组人脑利钠肽治疗患者.

摘要： 目的 探讨实时三维斑点追踪成像技术(RT-3D-STI)结合实时心肌声学造影(RT-MCE)技术评价心肌梗死后患者心肌存活性的临床应用价值.方法 选取25例根据心电图、心肌酶学及冠脉造影确诊,且成功进行冠状动脉血运重建术的心肌梗死患者.所有患者于术前1周内行RT-MCE检查,对心肌灌注结果进行半定量评价;分别于术前及术后6个月行二维超声分析左室各节段心肌进行室壁运动,根据术后室壁运动是否改善将室壁运动异常的心肌节段分为2组:存活心肌组和非存活心肌组;同时行RT-3D-STI技术测得左室心肌整体及各节段三维峰值长轴应变(3D-LPS)、环向应变(3D-CPS)、面积应变(3D-APS)及径向应变(3D-RPS)参数指标.结果 血运重建术前,存活心肌组3D-PLS、3D-PAS、3D-PCS、3D-PRS明显高于无存活心肌组(P<0.05).单参数ROC曲线分析结果显示,静息状态下,以术前3D-PAS≤-16.5%作为截断值判断心肌梗死后存活心肌的AUC为0.944,敏感度为91.3%,特异度为93.8%,明显高于其他应变值.多参数联合分析结果显示,三维应变参数联合判断心肌梗死后存活心肌的AUC为0.969,灵敏度及特异度分别为95.7%、90.6%.血运重建术前,RT-MCE评价存活心肌的敏感度及特异度分别为93.1%、68.8%,一致性分析得出Kappa值为0.645.结论 在静息状态下,RT-3D-STI技术预测心肌梗死后心肌存活的价值高于 RT-MCE技术,其中三维应变参数以3D-PAS≤-16.5%作为截断值判断心肌梗死后心肌存活性的价值最高,且两种技术联合应用能更好地评价心肌存活性.%Objective The aim of the present study was to investigate the value of predicting on the left ventricular myocardial viability in patients after myocardial infarction by real time three-dimensional speckle track-ing imaging(RT-3D-STI)combined with real time myocardial contrast echocardiography(RT-MCE). Methods 25 patients diagnosed with myocardial infarction by ECG,myocardial enzymes and coronary angiography and success-fully provided with coronary revascularization were recruited. Before revascularization,semi-quantitative evaluation of myocardial perfusion were determined by RT- MCE. After 6 months of follow-up,the patients were grouped into viable myocardium group and non-viable myocardium group based on whether having myocardial segmental wall mo-tion improvement or not after revascularization. Before and after revascularization,longitudinal peak strain(3D-GLPS),circumferential peak strain(3D-GCPS),radial peak strain(3D-GRPS)and area peak strain(3D-GAPS) by RT-3D-STI were measured for functional analysis. Results Before revascularization,3D-PLS,3D-PAS,3D-PCS,3D-PRS in myocardial viability group were significantly higher than those of non-viable myocardium group (P<0.05);the single parameter mode of ROC curve analysis showed that at resting state,the cutoff value of 3D-PAS which can assess myocardial viability was less than -16.5%,with a significantly higher AUC(0.944),sensi-tivity(91.3%),specificity(93.8%)than the other strains;the single technology mode of ROC curve analysis showed that 4D-SI had a AUC(0.969),sensitivity(95.7%)and specificity(90.6%). Before revascularization,the sensitivity and specificity of RT-MCE in assessing myocardial viability were 93.1% and 68.8%,the Kappa value was 0.645. Conclusion At resting state,RT-3D-STI has a higher value to predict myocardial viability after my-ocardial infarction than RT-MCE,among the three-dimensional strain parameters the cutoff value of 3D-PAS which can assess myocardial viability determines the highest value,and the combination of these two techniques is a more comprehensive method to assess myocardial viability.

摘要： Objective To investigate the effect of early application of recombinant human brain natriuretic peptide on myocardial viability and cardiac function in patients with anterior myocardial infarction (AMI) after emergency PCI.Methods A total of 100 patients were randomly divided into observation group (48 cases) and control group (52 cases).The control group received conventional coronary heart disease treatment;observation group in the conventional treatment of coronary heart disease based on the treatment of recombinant human brain natriuretic peptide.The levels of myocardial viability,cardiac function,BNP,CK-MB,cTnI and the incidence of cardiac adverse events were observed in both groups.Results The improvement rate of segmental improvement in the observation group(62.21％) was higher than that in the control group(46.11％),the difference was statistically significant (P＜0.05).After 1 month of treatment,LVESV,LVEDV,E/A were significantly decreased in both groups,and the observation group[(27.48± 1.35)ml/m2,(54.32±5.27)ml/m2,(0.76±0.03)] was significantly lower than that in the control group[(35.37±1.39)ml/m2,(67.49±6.25)ml/m2,(0.89±0.04)].Two groups of patients with LVEF,CI significantly increased,and the observation group [(59.54±3.01)％,(2.37±0.03)L/m2] was significantly higher than that in the control group[(52.07±3.44)％,(2.05±0.04)L/m2],the difference was statistically significant(P＜0.05).The levels of BNP,CK-MB and cTnI in the observation group were lower than those in the control group,the difference was statistically significant (P＜0.05).The incidence of adverse cardiac events in the observation group was lower than that in the control group for 6 months,the difference was statistically significant (P＜0.05).Conclusion Early application of recombinant human brain natriuretic peptide can improve the myocardial viability of patients with anterior wall AMI after emergency PCI,improve cardiac function and improve the incidence of cardiac adverse events,and have high clinical reference value.%目的 分析早期应用重组人脑利钠肽对前壁急性心肌梗死(AMI)急诊PCI术后心肌存活性及心功能的影响.方法 选择2014年5月至2016年10月在我院接受PCI治疗的前壁AMI患者100例为研究对象,随机分成观察组(48例)和对照组(52例).对照组接受常规冠心病治疗;观察组在常规冠心病治疗的基础上给予重组人脑利钠肽治疗.观察两组患者术后心肌存活性,心功能指标,BNP、CK-MB、cTnI水平以及心脏不良事件发生率.结果 观察组患者节段改善率(62.21％)均高于对照组(46.11％),差异有统计学意义(P＜0.05).治疗1个月后,两组患者LVESV、LVEDV、E/A明显下降,观察组显著低于对照组[(27.48±1.35)ml/m2比(35.37 ± 1.39)ml/m2,(54.32±5.27)ml/m2比(67.49±6.25)ml/m2,0.76 ±0.03比0.89 ±0.04];两组患LVEF、CI明显上升,观察组显著高于对照组[(59.54±3.01)％比(52.07±3.44)％,(2.37 ±0.03)L/m2比(2.05±0.04)L/m2],差异有统计学意义(P＜0.05);观察组BNP、CK-MB、cTnI水平低于对照组,差异有统计学意义(P＜0.05).治疗6个月后,观察组的心脏不良事件发生率低于对照组,差异有统计学意义(P＞0.05).结论 早期应用重组人脑利钠肽可提高前壁AMI急诊PCI术后患者的心肌存活性,改善心肌形变能力,保护心功能,降低心脏不良事件的发生率,具有较高的临床应用价值.

摘要： Objective To analyze the effect of myocardial perfusion on myocardial viability after percutaneous coronary intervention(PCI) in patients with acute ST-segment elevation myocardial infarction (STEMI).Methods A total of 63 acute STEMI patients who had PCI from March 2016 to March 2017 in Handan First Hospital were divided into group A[thrombolysis in myocardial infarction myocardial perfusion(TMP) grade 0-1,n =31] and group B(TMP grade 2-3,n =32).Myocardial infarction area(MIA) and myocardial viability were assessed by myocardial perfusion imaging 1 and 12 weeks after PCI.Location and number of coronary artery disease were analyzed.Results One-week MIA ratio assessed by resting myocardial perfusion imaging,1-week MIA ratio assessed after taking nitroglycerin and 12-week MIA ratio assessed by resting myocardial perfusion imaging in group B were significantly lower than those in group A[(26 ± 13)％ vs(39 ± 16)％,(20 ± 10)％ vs (34 ± 11) ％,(18 ± 13) ％ vs (37 ± 10) ％].Viable myocardium ratio in group B was significantly higher than that in group A [(5.43 ± 0.32) ％ vs (4.12 ± 0.15) ％] (P ＜ 0.05).Single vessel disease ratio and right coronary artery disease ratio in group B were significantly higher than those in group A[68.8％ (22/32) vs 35.5％ (11/31),46.9％ (15/32) vs 25.8％ (8/31)];multi-vessel disease ratio and left anterior descending branch disease ratio in group B were significantly lower than those in group A [9.4％ (3/32) vs 29.0％ (9/31),25.0％ (8/32) vs 51.6％ (16/31)] (P ＜ 0.05).Conclusion STEMI patients with TMP grade 2-3 show good myocardial viability after PCI.%目的 分析急性ST段抬高型心肌梗死(STEMI)患者经皮冠状动脉介入(PCI)术后不同心肌灌注水平对心肌存活性的影响.方法 选取2016年3月至2017年3月于河北省邯郸市第一医院行PCI的63例急性STEMI患者作为研究对象,按照心肌梗死溶栓试验心肌灌注(TMP)分级分为A组(31例,TMP分级0～1级)和B组(32例,TMP分级2～3级).观察2组患者PCI术后1周和12周的心肌灌注显像心肌缺损面积(MIA)和心肌存活情况,并比较2组冠状动脉病变部位及病变支数.结果 B组术后1周静态心肌显像、术后1周含服硝酸甘油心肌显像、术后12周静态心肌显像MIA百分比明显低于A组[(26±13)％比(39±16)％、(20±10)％比(34±1 1)％、(18±13)％比(37±10)％],心肌存活性明显优于A组[(5.43±0.32)％比(4.12±0.15)％],差异均有统计学意义(均P＜0.05).B组单支病变、右冠状动脉病变比例明显高于A组[68.8％(22/32)比35.5％(11/31)、46.9％ (15/32)比25.8％(8/31)],多支病变、左前降支病变比例明显低于A组[9.4％ (3/32)比29.0％ (9/31)、25.0％(8/32)比51.6％(16/31)],差异均有统计学意义(均P＜0.05).结论 STEMI患者行PCI术后TMP分级2～3级可提高患者的心肌存活性,其心肌恢复较快.

摘要： 目的 探讨三维斑点追踪技术结合腺苷负荷超声心动图评价心肌存活性的价值.方法 根据核素显像结果将41例心肌梗死患者的心肌划分为存活组及非存活组.应用三维斑点追踪技术分别检测患者静息状态及腺苷负荷状态下左心室各节段径向、纵向及环向收缩期峰值应变(Rs、Ls、Cs)及3D应变、面积应变(area strain,As).结果 (1) 41例患者的656个节段中,354节段静息状态下存在运动异常,其中非存活节段为131,存活节段为223.(2)静息状态下,非存活组与存活组相比较,As差异有统计学意义(P＜0.05),余参数无明显差异.(3)与静息状态下比较,腺苷负荷后存活组的Rs、Ls、3D strain、As有显著差异(P＜o.05),而Cs无显著差异(P＞o.05);非存活组的各指标差异无统计学意义.(4)腺苷负荷后,存活组与非存活组间的Rs、Ls、3D strain、As差异有统计学意义(P＜0.05),Cs无显著性差异(P＞0.05).(5)腺苷负荷后,以Rs增加值≥18.99％评价存活心肌的灵敏度为87.o％、特异度为93.9％;以Ls增加值≥22.25％评价存活心肌的灵敏度为90.8％、特异度为61.9％;以3D strain的增加值≥21.75％评价存活心肌的灵敏度为81.6％、特异度为90.1％;以As的增加值≥21.04％评价存活心肌的灵敏度为93.1％、特异度为95.5％.结论 三维斑点追踪结合腺苷负荷超声心动图可以有效地区分存活与非存活心肌.

摘要： 目的 探讨三维斑点追踪成像技术超声心动图评价心肌存活性的临床价值.方法 病例均为2010年4月至2012年12月间于南京医科大学附属南京医院心内科、心外科收住就诊患者,均根据心电图、心肌酶学及冠状动脉造影确诊为心肌梗死.受检患者静息状态下均存在不同程度的节段性室壁运动异常,其中部分患者已接受经皮腔内冠状动脉成形术和冠脉内支架置入术或冠状动脉旁路移植术,入选患者均排除糖尿病、心肌病及严重瓣膜病.选取图像清晰者进行图像采集,应用二维超声心动图观察入选45例心肌梗死患者心肌运动,二维斑点追踪成像及三维斑点追踪成像技术分别检测患者各节段径向、纵向及环向收缩期峰值应变(Rs、Ls、Cs)和(或)面积应变、3D应变.以核素心肌灌注/代谢显像结果作为金标准将各节段划分为存活组及非存活组心肌,并分析三维斑点追踪成像技术评定存活心肌的敏感度及特异度.所有测值均采用均数±标准差(-x±s)表示,两组间及组内比较采用t检验,特异度及敏感度分析采用受试者工作特征曲线(ROC)分析.结果 ①所观察的720个节段中,368个节段表现为运动异常,其中存活心肌节段204,梗死心肌节段164;②存活组与非存活组比较,二维应变Rs、Ls及Cs两组比较差异无统计学意义;③三维应变Cs于存活组及非存活组差异无统计学意义,Ls、Rs及3D应变、面积应变于非存活组较存活组应变值显著减低;④以Rs、Lss及3D应变、面积应变作为观察对象,Rs值为11.1％评价存活心肌的敏感度为95.1％,特异度为53.4％;Ls值为14.3％评判存活心肌的敏感度为65.2％,特异度为65.7％;3D应变值为17.4％评判存活心肌的敏感度为70.6％,特异度为77.2％;面积应变值为23.2％评判存活心肌的敏感度为91.5％,特异度为78.8％.结论 三维斑点追踪成像技术超声心动图能够作为临床提供有效区分存活与非存活心肌的新途径.%Objective To investigate the role of three-dimensional speckle tracking echocardiography (3D-STE) in providing a novel approach to assessing myocardial viability in patients with myocardial infarction (MI).Methods The subjects from the Department of Cardiology and the Department of Cardiac Surgery admitted from April 2010 through December 2012 were diagnosed as MI by electrocardiogram,myocardial enzymes and angiography.The clear imaging of angiography was selected out and collected.All patients had different degrees of segmental wall motion abnormalities,and some already had percutaneous transluminal coronary angioplasty and coronary stenting or coronary artery bypass grafting.Patients with diabetes,heart disease and severe valvular disease of heart were excluded.A total of 45 MI patients were checked with routine echocardiography,two-dimensional speckle tracking echocardiography (2D-STE) and 3D-STE.Then,radionuclide myocardial perfusion/metabolic imaging was served as a "golden standard" to distinguish the viable from nonviable myocardium in each patient within a day.In order to determine the most sensitivity and specificity threshold values of circumferential peak-systolic strain (Cs),longitudinal peak-systolic strain (Ls),radial peak-systolic strain (Rs),3D strain and area strain for viability detection from 3D-STE,the receiver operating characteristic curve was used to investigate the sensitivity and specificity of the detection of viable myocardium with strain parameters in the study.Comparisons between viable and non-viable groups were carried out with t test.Data were expressed as the mean value ± standard deviation (-x ± s).Results The ventricular wall motion abnormality by visual assessment was observed in 368 segments from 720 segments in 45 patients.Furthermore,204 segments were confirmed to be viable by radionuclide myocardial perfusion/metabolic imaging whereas the rest 164 segments were identified as nonviable among 368 abnormal segments.There were no significant differences in circumferential peaksystolic strain (Cs),longitudinal peak-systolic strain (Ls) and radial peak-systolic strain (Rs) by 2D-STE between viable and nonviable group.Compared with those in viable group,there wasn' t any difference in Cs,but Rs and Ls decreased significantly by 3D-STE in nonviable group.The 3D strain and area strain in absolute value decreased in nonviable group compared with viable group.According to 3D-STE,when Rs higher than 11.1％,the sensitivity was 95.1％ and the specificity was 53.4％ for identification of viable myocardium,whereas Ls higher than 14.3％ resulted in sensitivity of 65.2％ and a specificity of 65.7％.Besides,3D strain higher than 17.4％ had a sensitivity of 70.6％ and a specificity of 77.2％ for detection of viable myocardium,while area strain higher than 23.2％ allowed a sensitivity of 91.5％ and a specificity of 78.8％.Conclusions The 3D-STE might have potential reliability of myocardial viability detection in the patients with left ventricular dysfunction after MI.

摘要： 超声三维斑点追踪成像技术是一种评估心肌功能的新技术,该技术是基于二维灰阶组织成像基础上发展起来的非角度依赖性实用新技术,可通过识别图像的心肌回声斑点信号来追踪心肌运动轨迹,从多个方向对心肌节段应变进行评价,获取局部心肌径向、纵向及环向心肌形变信息,并实现三维空间的立体定位,可精确定量并定性评价心肌的局部及整体心肌应变力学改变,有效完成评价心肌的功能研究.斑点跟踪的应变和应变率对及早发现心肌功能障碍具有很高的敏感性及特异性,前瞻性检测心肌功能对冠状动脉粥样硬化性心脏病患者临床治疗决策上起着积极的指导作用,对患者实现阶段性疗效评估及长期预后预测均有着重要意义.综述阐述了斑点追踪二维及三维应变成像技术的基本概念,讨论了新的超声方法主要的临床应用价值及其存在的局限性和发展应用前景,这些都是目前国内外心血管超声研究的热点方向.%Three-dimensional speckle tracking is a new technology to evaluate myocardial function. It is a new imaging with angle independence which is based on gray-scale imaging. The spot signal myocardial echo with recognition image can track myocardial motion to e-valuate the segmental myocardial strain from multiple directions. Assessment of the echocardiography images has been recently described in radial peak strain, longitudinal peak strain and circumferential peak strain. It has been validated as methods to quantify regional myocardial function. To realize the space positioning of three-dimensional, strain can achieve precise quantitative and qualitative evaluation of myocardial changes. It can complete the function of effective evaluation of myocardium. Strain and strain rate of speckle tracking for the early detection of myocardial dysfunction which has higher sensitivity and specificity. Positive prospective detection of myocardial viability in patients with coronary heart disease has vital significance for clinical decision to the prediction and evaluation of curative effect. The paper introduces the basic concept of speckle tracking of two- and three-dimensional strain imaging. Clinical application value of ultrasound method for major new and existing limitations and development application prospect are discussed, which is one of the hot research in recent study of cardiovascular ultrasound.

摘要： 目的：采用低剂量多巴酚丁胺负荷下(DSE)心肌声学造影(MCE)评价冠心病患者心肌存活性。方法：30例经超声检查存在左室壁阶段性运动障碍的冠心病患者，行基础状态下和低剂量多巴酚丁胺(Dob)负荷状态下的心肌声学造影。将此30例患者行99Tc-MIBISPECT心肌核素显像，并将此结果作为判断存活心肌的标准。MCE造影图像采用QLAB软件对微泡在充盈曲线进行定量分析，求出各节段的A值、β值和A×β值。结果：负荷前后灌注指标比较，灌注增强，在本研究条件下，负荷后各节段A值以3.78,β值以0. 34和A×β值以1.37为截断点，诊断存活心肌的灵敏度、特异度分别是82.3%,77.6%和85.8%,79.7%及77.6%,87.5%。结论：采用低剂量多巴酚丁胺负荷下(DSE)心肌声学造影(MCE)法即低剂量多巴酚丁胺心肌声学造影法(DSE+MCE)可无创性的较为准确的检测冠心病患者的存活心肌。

摘要： 对冠心病患者心肌存活性进行了分析，采用低剂量多巴酚丁胺负荷下(DSE)心肌声学造影(MCE)进行评价，本研究使用QLab软件对30例患者的DSE + MCE图像进行定量分析，提出了A值以3. 78,β值以0. 34和A×β值以1. 37为截断点，诊断存活心肌的灵敏度、特异度分别是82.3 % ,77. 6%和85. 8 % ,79. 7%及77.6% ,87. 5%，对定量指标的具体数值进行了探讨。其中，A值代表血容量、β值代表血流速度、A×β值代表血流量。与既往研究中把A×β值小于0. 23作为判断坏死心肌及A×β值>1.5作为判断存活心肌的结果有一定差别，原因主要与入选患者冠状动脉狭窄程度、声学造影剂注射方式等因素有关。总之，DSE+MCE评价心肌灌注的客观性、可操作性和精确性方面更具优势。简便易行，无放射性损伤，可在床边进行，临床应用价值更大，可安全、无创性的较为准确的检测冠心病患者的存活心肌。

摘要： 本文就超声心动图检查中最新几种技术如药物负荷超声心动图试验,心肌灌注声学造影,声学密度定量技术,彩色室壁运动等在评价心肌存活性方面的研究进展进行介绍.

摘要： 尽管药物超声心动图是目前无创检测存活心肌的主要方法之一,但是目测室壁运动进行积分来评价心肌存活性的方法本身就存在着局限性,包括：半定量评价、对图像质量要求较高、需要操作者具有丰富的经验、其结果缺乏理想的客观性,等等。定量组织速度成像(QTVI)及组织追踪(TT)是在组织多普勒技术基础上发展起来的实用新技术。本文旨在探讨运用QTVI及TT定量评价心肌存活性的价值。

摘要： 一个称作miR-15家族(其包括miR-195)的微小RNA家族显示出在病理性心脏重塑期间上调并抑制细胞增殖和存活所需要的mRNA表达，结果损失心肌细胞。提供了在心脏中阻断miR-15家族的表达作为各式各样心脏病治疗方法的策略。

摘要： 本发明公开了一种中药组合物在制备促进骨髓间质干细胞在体存活和成心肌分化的药物中的应用。本发明还涉及该中药组合物在制备用自体骨髓间质干细胞治疗心血管疾病的药物中的应用。

摘要： 本发明涉及促进成年哺乳动物心肌细胞存活的体外培养方法及监测成年哺乳动物心肌细胞增殖的方法，所述体外培养方法为将新生哺乳动物的心肌细胞与成年哺乳动物的心肌细胞共培养；所述监测成年哺乳动物心肌细胞增殖的方法，包括以下步骤：(1)采用所述的体外培养方法培养成年哺乳动物心肌细胞，所述培养期间，利用活细胞工作站监测所述成年哺乳动物心肌细胞的增殖过程；(2)对步骤(1)中获得的新生成年哺乳动物心肌细胞进行免疫染色处理，鉴定所述新生成年哺乳动物心肌细胞的性质。该方法可显著延长成年哺乳动物心肌细胞的存活时间，利用活细胞工作站，对心肌细胞增殖进行直观记录，该方法为研究成年心肌细胞增殖及相关机制提供了有力工具。

摘要： 本发明公开了一种心肌梗死后缺血存活心肌的检测方法，调整

摘要： 本发明提供了一种心肌细胞存活的体外培养方法，具体包括以下内容：将离体的心脏组织使用缓冲液进行冲洗，然后转入培养皿中，剪成组织碎片；将组织碎片转入EP管中，使用II型胶原酶与胰蛋白酶混合液进行初次消化，然后加入混有DNase I的II型胶原酶溶液进行多次短时消化；完成后，吸取EP管中的上清液后并将上清液放置于离心管中；进行离心并除去上清，收集细胞沉淀，再加入完全培养基，进行心肌细胞重悬等。本发明本发明培养的心机细胞搏动时间早、存活时间长、在较长的培养时间内维持高的细胞纯度，心肌成纤维细胞、纯度高、得率高。

摘要： 本发明涉及提高心肌细胞聚集体的长期保存有效性的方法，在利用本发明的方法的情况下，可以冷冻保存心肌细胞聚集体，从而可以扩大利用多能干细胞的类器官制备及利用类器官的新药开发等三维细胞结构体研究范围，不仅如此，在向体内移植后发生的低氧条件下的细胞存活率得到提高，从而期待可有效地用于细胞治疗剂开发等相关技术。

摘要： 本发明涉及一种存活心肌评价方法，所述评价方法是基于门控SPECT心肌灌注显像心肌功能结合心肌灌注进行评价，首先采用门控SPECT心肌灌注显像获取检查图像，重建得到左心室短轴、水平长轴及垂直长轴图像；然后对左心室心肌节段的血流灌注摄取进行半定量评分，判断灌注减低节段；再定量评价得到左室心肌每个节段的血流灌注摄取比；再对重建后的门控SPECT心肌灌注显像进行分析，分别获得每个心肌节段的室壁运动和增厚率评分，最后将上述参数代入评价模型进行计算。本发明基于门控SPECT心肌灌注显像心肌功能结合心肌灌注进行评价特别适用于冠心病心力衰竭患者血运重建术前评价存活心肌，指导临床治疗决策。

摘要： 本发明公开了一种18F‑FDG PET存活心肌显像调糖用血糖监测智能装置，包括：信息采集模块：所述信息采集模块用于对受检者的血糖信息进行采集；电子传输模块，所述电子传输模块用于对采集到的受检者的血糖信息生成血糖‑时间变化曲线；显示模块，所述显示模块对由电子传输模块生成的血糖‑时间变化曲线进行显示；阈值对比模块，所述阈值对比模块设定受检者的低血糖的危险阈值。本发明，根据血糖‑时间变化曲线，在整个葡萄糖负荷过程中实时关注患者血糖情况，待其明显升高大于阈值时给予一定剂量的胰岛素，然后在血糖下降曲线斜率最大时注射18F‑FDG，最大程度地调动存活心肌对于葡萄糖的摄取，提高图像质量，确保显像成功；且可避免患者短时间内多次测量血糖被扎的疼痛及不适感，增加患者依从性。

摘要： 本发明提供一种乏氧存活心肌的显像增强设备，在心脏跳动同一节点对SPECT设备显像图像进行采集，再使用用多幅重合的图像处理技术对采集的图像进行重合，加强了心脏病灶区的图像显示强度，增加了心脏病灶边缘的锐度，提高了乏氧存活心肌的成像质量。

摘要： 本发明公开了阿托伐他汀在促进骨髓间质干细胞移植后在体存活和向心肌细胞分化中的应用。本发明还涉及该药在用于自体骨髓间质干细胞治疗心血管疾病中的应用。