摘要：
目的 明确尤文联合地塞米松对大鼠急性肺损伤模型的效果及其相关机制. 方法 将96 只SD大鼠分别于气管内滴注LPS制备急性肺损伤模型,随机分为溶媒对照组( LPS组)、英脱利匹特组(ω-6 组)、尤文组(ω-3组)、地塞米松组( DXM组)、英脱利匹特+地塞米松组(ω-6 +DXM组)、尤文+地塞米松组(ω-3 +DXM组) ,分别给予相应的干预处理3 d后,HE染色检测肺组织病理改变,测定PaO2 及肺组织湿/干重比( W/D) ,ELISA法检测BALF中相关炎症因子的含量. 结果 LPS组与ω-6组大鼠肺泡隔增厚,肺组织出血、水肿及大量炎性细胞浸润,肺组织W/D比值明显高于其他各组;给予地塞米松或尤文后,模型大鼠死亡率降低,肺损伤程度减轻,PaO2 升高,BALF中TNF-α、IL-1β、IL-6含量降低( P<0. 05 ). ω-3 组、DXM组、ω-6 +DXM组之间各检测指标无明显区别,而ω-3+DXM组肺组织基本正常,W/D比值最低,PaO2 最高,BALF中TNF-α、IL-1β、IL-6含量明显低于其他各组;给予尤文后BALF中IL-10 明显增高,ω-3 组与ω-3 +DXM组BALF中IL-10 明显高于其他各组,差异均有统计学意义( P<0. 05 ). 结论 尤文联合地塞米松可通过调控TNF-α、IL-1β、IL-6 及IL-10等相关炎症因子的表达,减轻肺损伤.%Objective To explore the effects of omeganven combined with dexamethasone on the acute lung injury of rat model and the mechanism. Methods Ninety-six SD rats were treated with lipopolysaccharide ( LPS) by intratracheal instillation to induce acute lung injury,and they were randomly divided into control group ( LPS group) , intralipid group (ω-6 group) ,omeganven group (ω-3 group) ,dexamethasone group ( DXM group) ,intralipid+dexa-methasone group (ω-6+DXM group) and omeganven+dexamethasone group (ω-3+DXM group). After 3 d of cor-responding treatment,the pathologic changes in lung tissue section,wet/dry weight ratio (W/D) of lung tissue and PaO2 were measured. The concentration of TNF-α, IL-1β, IL-6 and IL-10 in broncho-alveolar lavage fluid ( BALF ) were respectively measured by ELISA. Results In rats of LPS group and ω-6 group,alveolar septum thickening,lung hemorrhage,edema and inflammatory cell infiltration in lung tissue were easily seen,and W/D ratio of lung tissue was significantly higher than that of another groups. Among rats of ω-3 group,DXM group and ω-6 +DXM group,there was no obvious difference in pathological changes,and the level of TNF-α,IL-1βand IL-6 in BALF,however,the inde-xes decreased significantly after treatment in DXM group and ω-3 group. In rats of ω-3+DXM group,the structure of lung tissue was almost normal,and the W/D ratio and the levels of TNF-α,IL-1β and IL-6 in BALF were the lowest (P<0. 05). Furthermore,the level of IL-10 in BALF increased remarkably after treatment with omeganven,and it was obviously higher in rats of ω-3 group and ω-3 +DXM group ( P <0. 05 ) . Conclusion Omeganven combined with dexamethasone can relieve lung injury through regulating the expression of inflammatory factor in BALF such as TNF-α,IL-1β,IL-6 and IL-10 in rats with acute lung injury.
