摘要：
[Objective] To study the effects of temperature and host on Cacopsylla citrisuga growth and de-velopment.[Method]C. citrisuga was parasitized on healthy or Candidatus Liberibacter asiaticus-infected Citrus limon in the incubators under simulative natural environment. The developmental periods, survival rates of different life stages, preoviposition period and fecundity of C. citrisuga were analyzed and com-pared. Adult survival rates were compared for C. citrisuga parasitized on C. limon, Murraya exotica and Citrus sunki under 15, 20 and 25°C. [Result]Under 15 °C, 70% relative humidity and on healthy C. limon, the mean duration of C. citrisuga egg was 9. 38 days, the durations from 1st to 5th instar nymph were 8. 80, 6. 66, 6. 00, 6. 76 and 5. 94 days respectively, the preoviposition period was 16. 2 days, the total duration of generation was 59. 74 days and the survival rate of generation was 28. 34%. For C. cit-risuga parasitized on Ca. L. asiaticus-infected C. limon, the durations of 2nd instar, 4th instar and total nymph stages significantly decreased, the preoviposition period significantly increased, and the survival rates of 4th instar, total nymph stages and generational period significantly decreased compared to those on healthy lemon. There was no significant difference in fecundity when the same origin psyllid females were laying eggs on healthy and infected C. limon, but the fecundity of the subsequent generation females emerged from healthy C. limon was significantly higher compared to those from infected C. limon. The survival rate of C. citrisuga adults was the highest on C. limon comparing three hosts under the same temperature, and was the highest under 15°C comparing different temperatures when reared on the same host plant species. There were 81. 67% adults survived after 20 days of being parasitized on C. limon under 15 °C. The nymph developed faster when parasitized on Ca. L. asiaticus-infected C. limon, but the pathogen negatively influenced the survival and reproduction of C. citrisuga. [Conclusion]C. cit-risuga can accomplish the whole generation with high survival rate under the above conditions, which can be applied to breed C. citrisuga indoor, and can be used for other studies related to C. citrisuga growth and Huanglongbing mechanisms.%【目的】研究温度和寄主对柚喀木虱Cacopsylla citrisuga生长发育的影响。【方法】模拟柚喀木虱的自然生存环境,用人工气候箱研究其在柠檬Citrus limon上繁殖时各虫态的发育历期、存活率、成虫产卵前期和产卵量,同时比较其在黄龙病柠檬苗上饲养时上述生物学指标的变化；研究15、20和25°C条件下柠檬、九里香Murraya exotica和酸橘Citrus sunki对柚喀木虱成虫存活率的影响。【结果】在15°C、相对湿度70%条件下柚喀木虱在健康柠檬上平均卵历期为9．38 d,1~5龄若虫历期分别为8．80、6．66、6．00、6．76和5．94 d,产卵前期为16．2 d,世代历期为59．74 d,世代存活率为28．34%；而在带病柠檬上2龄、4龄和总若虫历期显著缩短,产卵前期显著延长,4龄、总若虫期存活率和世代存活率显著下降。来源相同的成虫在健康和黄龙病柠檬苗上的产卵量无显著差异,但在病苗和健苗上分别饲养1代后,从健苗上羽化的成虫产卵量显著高于病苗。在相同的温度条件下,用柠檬饲养柚喀木虱成虫存活率最高；而在相同的寄主条件下,温度为15°C时存活率最高；成虫在柠檬上15°C条件下饲养20 d后存活率仍达81．67%。寄主感染黄龙病使柚喀木虱若虫的发育历期缩短,但对其存活率和繁殖造成不良影响。【结论】该饲养条件下的柚喀木虱能顺利完成整个世代且能获得较高存活率,该饲养条件可用于室内大量繁殖柚喀木虱,并进一步应用到柚喀木虱的生活习性和黄龙病病理研究中。
