tomato

摘要： Tomato is one of the most important fruit crops in the world which is consumed in a variety of ways. The high cost of chemical fertilizers has led to some farmers turning to alternative methods of production, such as the use of bio-fertilizers. Most organic waste may be changed into fertilizer at a low cost of production. The objectives of this study were to determine: 1) the effect of the application of bio-compost on growth, yield, and quality of tomato;2) to determine the effects of different bio-compost applications on growth, yield and quality of tomato. The field was marked out into eight blocks. It consisted of four treatments with two replications per treatment. The bio-fertilizer was applied to plots and incorporated into the soil. This study showed that the application of bio-fertilizers made from food waste only, cow dung only, and food waste and cow dung improved plant growth, number of leaves, plant height and fruit yield. However, the differences were not statistically significant except for the number of leaves. The study also revealed that the application of bio-fertilizer lowered the number of days to flowering, fruiting, and ripening by 50% as compared to the control group. Promoting the adoption and use of bio-compost made from different waste stream hold the promise to increase tomato production.

摘要： Seedling vigor in tomato and paprika is affected by variety. Genotype selection under environmental stresses and its effects on seedling vigor was investigated. During the year 2021, the study was carried out and it revealed an appropriate selection for open field gardeners. The selection of two drought-tolerant, and one non-drought tolerant cultivars was the initial stage. Six commercial genotypes were evaluated (three for tomato and three for paprika). The second phase involved planting cultivars till the cotyledon leaves phase, and the first set of true leaves. Seedlings were investigated under simulated environmental stresses of non-ideal temperatures, low humidity, closed spacing, minimum light dose, nutrient-deficient water, and spraying a lower dosage of salicylic acid. The evaluation of seedling’s growth was performed by measuring germination percentage, seedling length, shoot length, root length, seedling fresh weight, seedling dry weight, and seedling vigor index. Tomato (Mobil) and paprika (Carma) seedlings outperform other varieties, possibly because of the variety’s vigor under various stress conditions. These findings reveal that tomato (Mobil) and paprika (Carma) had a positive impact on development and may be raised under optimal conditions of nurseries and then be transferred to open-air environmental and biological exposed conditions in Hungary.

摘要： Tomato mosaic virus(ToMV)drastically affects the tomato production worldwide.To deal with this problem,breeding of ToMV-resistant hybrids/varieties is the ultimate need and most successful approach.In wild tomato species,three dominant ToMV-resistant genes(Tm-1,Tm-2 and Tm-2^(2))were identified and the World Vegetable Center developed few fresh market tomato lines resistant to ToMV by the introgression of these genes.Recently at Nuclear Institute for Agriculture and Biology,Faisalabad,Pakistan a breeding programme was initiated to develop high yielding and ToMV tolerant hybrids using these lines.Current study was performed to screen elite F1 hybrids carrying Tm gene along with their parents against ToMV using mechanical inoculation,confirmation of the virus using DAS-ELISA and marker assisted selection of hybrids.Out of 28 hybrids and 17 parent accessions/genotypes,eight hybrids and five accessions were found to be highly resistant and the virus was not detected in DAS-ELISA.Five hybrids were resistant,nine hybrids and four genotypes were tolerant,while the remaining six hybrids and eight genotypes were susceptible.For the confirmation of Tm-2^(2) gene,the tomato hybrids and their parents were subjected to molecular analysis using cleaved amplified polymorphic sequence(CAPS)primers.The result of CAPS markers for the confirmation of Tm-2^(2) gene was found consistent with phenotypic data of the inoculated tomato genotypes/hybrids.Higher phenolic content,total soluble proteins,better CAT and SOD activities were positively correlated with resistance.Screening results based on phenotype,biochemical and molecular marker data indicate that hybrids carrying Tm-2^(2) gene are good sources of resistance against ToMV.

摘要： The use of natural hydrogels in agriculture provides solutions to many problems without threatening the environment. This study aims to evaluate the potential impact of environmentally friendly hydrogels (pectin, starch and pectin + starch) in reducing the negative effects of drought stress on tomato yield and quality. Two different peels (orange peel and banana peel) are used to prepare environmentally friendly hydrogels. The water retention efficiency of hydrogels has been studied. Greenhouse experiment for tomato under drought stress was conducted during 2019. These hydrogels were used under several level of irrigation (100%, 75%, and 50% FC), soil without hydrogel was used as a control. The results showed that the eco-friendly hydrogels (starch, pectin, and pectin + starch) had capacity retention of water for a long time. The obtained data from the greenhouse experiment showed that the eco-friendly hydrogel showed a positive effect on retention of water and increase the soil moisture content compared to control. The highest increase was observed at pectin + starch treatment. This increase reached to 2.8-, 2.4- and 2.0-fold for 100%, 75% and 50% FC compared to the control. Eco- friendly hydrogel application under different drought conditions led to improve yield and quality of tomato fruits. Moreover, conversion of agricultural wastes to hydrogels and the use of these eco-friendly materials instead of synthetic hydrogels are necessary to utilize the limited natural resources and decrease the harmful impact of agricultural wastes on the environment and pave the way for the transition to a sustainable agriculture system.

摘要： The knowledge generated from the identification of plant promoters has been very important for plant biotechnology development. The use of promoters in transgenic plants allows a reasonable level of regulating protein expression. With the application of reporter genes, such as gusA (uidA,) the production of a colored protein, β-glucuronidase, can be detected and measured both qualitatively and quantitatively, and the activity of the promoter can be assessed. In this work we use a promoter of an abundant banana fruit protein gene Musa acuminata Acidic Chitinase class III a monocot species, to drive expression of gusA in a dicot species, like tomato. We evaluated the monocot promoter capabilities by localizing and quantifying β-glucuronidase (GUS) expression through fluorometric assays during tomato fruit ripening. Our results suggest that this promoter could be used for specifically strong fruit protein expression in dicot plants.

摘要： The abscisic acid(ABA)receptor PYR/PYL/RCAR,located upstream of the ABA signaling pathway,recognizes ABA signals and initiates the primary process of signal transduction.In our previous study,we found that the PYL4 gene in tomato plants was significantly upregulated after exogenous treatment with the hormone ABA.The present study used virus-induced gene silencing(VIGS)to further elucidate the role of the PYL4 gene in response to drought in tomato(Solanum lycopersicum)plants.Under the drought treatment,the SlPYL4-silenced plants wilted faster than the control plants did.Physiological indicators showed that the total reactive oxygen species(ROS)content and superoxide dismutase(SOD),peroxidase(POD)and catalase(CAT)activities of the SlPYL4-silenced plants were lower than those of the control plants after 3 and 6 h of drought stress treatment.After 3 h of drought treatment,hydrogen peroxide(H_(2)O_(2)) and superoxide anion(O_(2)^(−).)accumulatedmuch more in the leaves of the SlPYL4-silenced plants than in the leaves of the control plants.These results indicated that PYL4 plays a positive role in drought tolerance in tomato plants.Our results also suggest that SlPYL4 is located upstream of the ABA pathway to regulate the expression of PP2C,SnRK,and AREB/ABF genes.

摘要： Cell cycle regulation plays a critical role in plant growth and development.In this study,the role of a tomato cell cycle gene SlCycB1 has been characterized.Expression analysis revealed that SlCycB1 was mostly expressed in stem,root,and leaves,with relative lower expression in flower and fruit.Tomato plants overexpressing SlCycB1 exhibited a reduction in cell number and increased cell size leading to the growth retardation.Furthermore,yeast two-hybrid analysis and bimolecular fluorescence complementation revealed that SlCycB1 interacted with histone H3.2,an essential component of the nucleosome.Histone H3.2 was transcriptionally up-regulated in the SlCycB1 overexpressing tomato lines.Furthermore,the overexpression of histone H3.2 in transgenic plants showed similar phenotypes to SlCycB1 overexpressing lines.Based on these findings,we concluded that SlCycB1 overexpression altered tomato architecture in association with histone H3.2.

摘要： The combined stress of high temperature and high relative air humidity is one of the most serious agrometeorological disasters that restricts the production capacity of protected agriculture.However,there is little information about the precise interaction between them on tomato fruit quality.The objectives of this study were to explore the effects of the combined stress of high temperature and relative humidity on the sugar and acid metabolism and fruit quality of tomato fruits,and to determine the best relative air humidity for fruit quality under high temperature environments.Four temperature treatments(32°C,35°C,38°C,41°C),three relative air humidity(50%,70%,90%)and four duration(3,6,9,12 d)orthogonal experiments were conducted,with 28°C,50%as control.The results showed that under high temperature and relative air humidity,the activity of sucrose metabolizing enzymes in young tomato fruits changed,which reduced fruits soluble sugar content;in addition,enzyme activities involved phosphopyruvate carboxylase(PEPC),mitochondria aconitase(MDH)and citrate synthetase(CS)increased which increased the content of organic acids(especially malic acid).Eventually,vitamin C,total sugar and sugar-acid ratio decreased significantly,while the titratable acid increased,resulting in a decrease in fruit flavor quality and nutritional quality in ripe fruit.Specifically,a temperature of 32°Cand a relative air humidity of 70%were the best cultivation conditions for tomato reproductive growth period under high temperature.Our results indicating that fruit quality reduced under high temperature at the flowering stage,while increasing the relative air humidity to 70%could alleviate this negative effect.Our results are benefit to better understand the interaction between microclimate parameters under specific climatic conditions in the greenhouse environment and their impact on tomato flavor quality.

摘要： Tomato(Solanum lycopersicum L.)is called‘the poor man’s orange’due to its low price and improved nutritional values.An experiment was conducted to study the breeding potential of some exotic tomato lines by assessing various qualitative and quantitative traits conferring yield and quality attributes.Among the qualitative traits,greater variability was observed for growth type,stem hairiness,and fruit shape and size.A determinate growth habit was observed in the genotype AVTO9802 while the genotype AVTO0102 produced yellow color fruits.A significant(p≤0.01)variation was also observed for the studied quantitative traits.Based on yield and traits attributed to yield,the genotypes AVTO0314,GPB0107,GPB0120 and AVTO9802 were selected as promising genotypes.The differences between the genotypic and phenotypic coefficients of variation(GCV and PCV)of the studied quantitative traits were very low.This suggests that the apparent variation was mainly due to the genotypes.The higher GCVand PCV values were observed for the number of primary branches plant^(−1)(NPB),number of fruits cluster^(−1)(NFC),individual fruit weight(IFW)and total soluble solids(TSS).High heritability was recorded for all quantitative traits in a broad sense.However,the individual fruit diameter showed the highest heritability(99.56).The highest(102.75)genetic advance(GA)was observed for the number of fruits plant^(−1)(NFP).High heritability coupled with high GA as percentage of mean were recorded for the traits NFP,NFC,fruit yield plant^(−1)(FYP)and IFW.FYP showed a significant positive correlation with NFC(0.714^(***))and a negative correlation with days to the first harvest(−0.539^(***))and plant height(−0.492**).Principal component analysis revealed that the first four components explained 78.5%of the total variation among the genotypes.Thus,the promising genotypes(AVTO0314,GPB0107,GPB0120,AVTO9802 and AVTO0102)isolated from this study can be used for developing high-yielding and high-quality tomato varieties.

摘要： Tomato is an important vegetable crop and fluctuating available soil phosphate(Pi)level elicits several morphophysiological responses driven by underlying molecular responses.Therefore,understanding these molecular responses at the gene and isoform levels has become critical in the quest for developing crops with improved Pi use efficiency.A quantitative time-series RNA-seq analysis was performed to decipher the global transcriptomic changes that accompany Pi starvation in tomato.Apart from changes in the expression levels of genes,there were also alterations in the expression of alternatively-spliced transcripts.Physiological responses such as anthocyanin accumulation,reactive oxygen species generation and cell death are obvious 7 days after Pi deprivation accompanied with the maximum amount of transcriptional change in the genome making it an important stage for in-depth study while studying Pi stress responses(PSR).Our study demonstrates that transcriptomic changes under Pi deficiency are dynamic and complex in tomato.Overall,our study dwells on the dynamism of the transcriptome in eliciting a response to adapt to low Pi stress and lays it bare.Findings from this study will prove to be an invaluable resource for researchers using tomato as a model for understanding nutrient deficiency.