摘要：
Transcriptional regulator Tango (Tgo)plays crucial roles in insect development,including neurogenesis, hematopoiesis,sex determination and gut development.In this study,a full-length cDNA of Tango was cloned in Nilaparvata lugens .qRT-PCR and RNA interference (RNAi)were further used to analyze the expression pattern and function role,respectively.Our results showed that NlTgo contained a 2007-bp open reading frame (ORF),encoding 669 amino acid residues.Sequence alignment showed that NlTgo shared an identity of 68% with Pediculus humanus . Phylogenetic analysis suggested that NlTgo was closely related to the Tango proteins from Acyrthosiphon pisum , P yrrhocoris apterus and Cimex lectularius .Expression profile revealed that NlTgo expression was higher in the first-and second-instar larvae than that in eggs.Furthermore,the expression level of NlTgo was higher in ovary than that in integument.Knocking down of NlTgo ,in the 4th-instar nymph,was performed by double-stranded RNA (dsRNA) targeting NlTgo .It was found that the expression level of NlTgo ,4 days after injection,was significantly decreased by 77%,compared with control.Furthermore,nymphs died due to the abnormal molting,and the survival rate was only 23%,significantly lower than control group (98%).The results suggest that NlTgo is involved in the development of N .lugens and can serve as a potential target for controlling the brown planthopper.%转录因子 Tango(Tgo)在昆虫神经元发生、血细胞生成、性别决定、肠道发育等过程中发挥重要作用.克隆了褐飞虱Tango 基因(NlTgo ),应用荧光定量 PCR 和 RNAi 探索了 NlTgo 在褐飞虱中的表达动态和生物学功能.结果表明,NlTgo的开放阅读框为2007 bp,推测编码669个氨基酸残基.多序列比对表明 NlTgo 与已知的 Tgo 高度同源,其中与人体虱(Pediculus humanus corporis )Tgo 的一致性达68％.系统进化分析表明,NlTgo 与豌豆长管蚜(Acyrthosiphon pisum )、始红蝽(P yrrhocoris apterus )和臭虫(Cimex lectularius )的 Tgo 在系统发育树中形成一个类群.时空表达谱表明 NlTgo 在褐飞虱1龄、2龄时期高表达,在卵期表达量较低；在卵巢中表达量最高,在体壁的表达量较低.RNAi 结果表明,注射dsNlTgo后4 d,NlTgo 的表达量较空白对照组显著降低了77％,并导致若虫不能正常蜕皮而死亡.其中,处理5 d 后褐飞虱的存活率仅为23％,显著低于注射 dsGFP 的对照组(98％).结果显示,NlTgo 与褐飞虱的生长发育有关,可作为防治褐飞虱的潜在靶标.
