soybean

摘要： Plant height is an important agronomic trait, which is governed by multiple genes with major or minor effects. Of numerous QTLs for plant height reported in soybean, most are in large genomic regions, which results in a still unknown molecular mechanism for plant height. Increasing the density of molecular markers in genetic maps will significantly improve the efficiency and accuracy of QTL mapping. This study constructed a high-density genetic map using 4 011 recombination bin markers developed from whole genome re-sequencing of 241 recombinant inbred lines(RILs) and their bi-parents, Zhonghuang 13(ZH) and Zhongpin 03-5373(ZP). The total genetic distance of this bin map was 3 139.15 cM,with an average interval of 0.78 cM between adjacent bin markers. Comparative genomic analysis indicated that this genetic map showed a high collinearity with the soybean reference genome. Based on this bin map, nine QTLs for plant height were detected across six environments, including three novel loci(qPH-b_11, qPH-b_17 and qPH-b_18). Of them, two environmentally stable QTLs qPH-b_13 and qPH-b_19-1 played a major role in plant height, which explained 10.56-32.7% of the phenotypic variance. They were fine-mapped to 440.12 and 237.06 kb region, covering 54 and 28 annotated genes, respectively. Via the function of homologous genes in Arabidopsis and expression analysis, two genes of them were preferentially predicted as candidate genes for further study.

摘要： This study aims to determine the ability of CM(Cow Manure)combined with RHA(Rice Husk Ash)to provide a significant effect on the growth and production of soybean planted in an ultisol.It was conducted in a pot experiment at the Experimental Site of the Faculty of Agriculture,Sriwijaya University,Indralaya,Indonesia between September 2019 and February 2020 using completely randomized design factorial with two factors which were two rate levels of RHA at 3.75 and 7.5 tons·ha^(-1) and three rate levels of CM at 0,10,and 20 tons·ha^(-1).It is important to note that each combination was repeated three times,thereby,leading to a total of 18 pots.The variables studied were plant growth and yield which are represented by plant height,the total number of pods,number of filled pods,seed weight per plant,and soybean produced.The results showed that CM combined with RHA was effective in increasing nutrient availability and reducing soil acidity.It was discovered that CM had a significant effect on soil pH,plant height,the total number of pods,number of filled pods,seed weight per plant,and soybean produced while RHA did not have any significant difference on all the variables observed.However,the combination of 10 tons·ha-1 CM with 3.75 tons·ha-1 RHA was observed to be the best combination treatment to increase the growth and production of soybean in ultisol as indicated by its ability to produce 2.58 tons·ha^(-1) soybean.

摘要： Soybean is an oilseed crop legume cultivated for its benefits as a source of protein to human or animal food. The cultivation of soybean will promote the diversification of income sources for rural population. Like other crops, soybean flowers are visited by insects. It is expected that within the biodiversity conservation program, anthophilous insects including bees, generally increase fruit and seed yields of many plant species. Therefore, the effect of insect pollinators and compost on growth and yield parameters of Glycine max was assessed for two cropping seasons (2018 and 2019) in the field. The experiment was set up in a complete randomized block design with three treatments: subplots applied with compost;subplots applied with fertilizer-NPK;subplots applied neither with compost, nor with fertilizer-NPK. Two other treatments were designed by plants with flowers protected against insects or flowers pollination free. Results indicate that root nodules formed by soybean plants in plots that received compost were significantly higher (P < 0.001) than those from positive and negative controls. During the 2018 and 2019 cropping seasons, 948 and 593 visits from five insect species were recorded on G. max flowers respectively. Lipotriches collaris was the most insect species frequently observed in the field, with 44.20% and 43.34% visits yearly respectively. The synergistic effect of insects and compost increased the number of seeds per pod by 28.27% and the percentage of normal seeds by 24.47%. Hence, applying Glycine max seeds at sowing with compost and in an environment with hives close to field could be recommended in agricultural development programs of farmers for a sustainable improvement of pods and seed yield of this valuable crop.

摘要： Arbuscular Mycorrhizal Fungi (AMFs) could be used to sustainably improve crop yields. The present study evaluated the diversity of AMF species associated with soybean (Glycine max L. Merill) in main soybean-producing areas in Benin. Composite soil samples from 13 production areas at a rate of 04 villages per production areas were collected. A spore trapping device was set up to reveal the diversity of spores. The physical and chemical properties of the soils, the frequency and intensity of mycorrhization of roots, and the diversity of AMF spores were determined in the soil samples following trapping. As result, eight morphotypes belonging to four genera: Glomus, Acaulospora Gigaspora and Disversispora and three families: Diversisporales, Glomérales and Paraglomérales were observed. An important variability of spore densities was observed from one production areas to another with a higher abundance in the production areas of Copargo estimated at 3584 spores/100g soil. The biological diversity indexes as Shannon (0.0311), Simpson (0.0204) and Hill (0.0235), varied significantly (p < 0.05) from one production areas to another. There was significant correlation between the parameters studied, particularly between the physico-chemical parameters of the soils and between the physico-chemical parameters and the biological diversity indexes. For the mycorrhization parameters, the mycorhization frequencies did not vary from one production areas to another, unlike the intensities, which significantly varied from one production areas to another (2.31% to 24.62%). Finally, this study revealed that the physico-chemical parameters of the soils had an influence on the other parameters studied. Moreover, there were an abundance and a significant diversification of AMFs associated with soybean in the different production areas, which are influenced by certain physico-chemical soil parameters.

摘要： Forage soybean is an important source of high protein forage.Variety screening and breeding not only can solve the adjustment of agricultural planting structure,but also can provide a large amount of high-protein forage material,and effectively solve the problem of a serious shortage of high-protein forage in herbivorous animal husbandry in China.In this study,the feeding-type soybean strain HN389 was selected as experimental material,with three planting densities of 270000,405000 and 540000 plants•hm^(-2) and three cutting periods of the initial pod stage(R1),the initial grain stage(R2)and the early mature stage(R3)were set to determine the yield and feeding quality,in order to obtain the best planting density and harvest time of the variety.The results showed that in forage soybean strain HN389 at the R1 and R2 stages,plant height increased with increasing planting densities,while fresh and dry weight per plant decreased with increasing planting densities,and there was no significant difference at the R3 stage.The yield of hay at the R1,R2 and R3 stages increased firstly and then decreased with the increase of planting densities,and the yield per hectare was R3>R2>R1.The order of contents of crude protein(CP),neutral detergent fibers(NDF)and acid detergent fiber(ADF)in feeding quality of HN389 were R2>R1>R3,and ether extract(EE)content order was R3>R1>R2,and there was no significant difference among different groups.Two principal components were extracted from five forage indexes including CP,NDF,ADF,EE and fresh grass yield by principal component analysis.The cumulative contribution rate of principal components 1 and 2 was 90.053%,and their characteristic values were 3.617 and 0.885,respectively.After a comprehensive evaluation,harvested at the R3 stage and the density was 405000 plants•hm^(-2),HN389 had the highest comprehensive score of 0.344,yield of 38035.53 kg•hm^(-2),CP,NDF,ADF and EE contents of 17.61%,17.61%,21.54%and 3.81%,respectively.

摘要： [Objectives]This study was conducted to explore the mechanism of soybean cytoplasmic-nuclear male sterility.[Methods]With soybean cytoplasmic-nuclear male sterile line JLCMS9 A and its homotype maintainer line JLCMS9 B as experimental materials,the activity of superoxide dismutase(SOD),peroxidase(POD)and catalase(CAT),malondialdehyde(MDA)content,starch content,soluble protein content,soluble sugar content and free proline content in flower buds,alabastrums and mature flowers were determined,and the contents and changes of auxin(IAA),gibberellin(GA3),isopentenyl adenosine(iPA)and abscisic acid(ABA)at the three stages were analyzed.[Results]The activity of SOD and CAT and the contents of MDA and free proline in the sterile line at the flower bud stage were lower than those of the maintainer line,but the opposite was true at the alabastrum stage and the flowering stage,and their values were higher than those of the maintainer line;the POD activity of the sterile line was significantly lower than that of the maintainer line at the flower bud stage,and the opposite was true at the alabastrum stage and the flowering stage,and its values were higher than those of the maintainer line;and the starch content and soluble sugar content of sterile line 9 A showed an overall upward trend,and were significantly lower than those of the maintainer line 9 B at the alabastrum stage and the flowering stage.During the whole development process of floral organs,the content of IAA in sterile line 9 A showed a trend of first increasing and then decreasing,and the content of iPA increased gradually.The contents of hormones in the sterile line were lower than those in the maintainer line.The ratios of IAA/ABA,IAA/GA3,IAA/iPA and ABA/GA3 in the sterile line were significantly different from those in the maintainer line.It is inferred that the abnormal physiological characteristics of floral organ development are related to the cytoplasmic-nuclear male sterility of soybean.The alabastrum stage may be a critical period for the occurrence of abnormal physiological and biochemical indexes in the floral organs of soybean cytoplasmic-nuclear male sterile lines.[Conclusions]This study provides a theoretical basis for the breeding of fine sterile lines of soybean and the research on the mechanism of sterility.

摘要： Phytophthora sojae infection severely impairs soybean production. We previously identified a dirigent protein, Gm DRR1(Glycine max Disease Resistant Response 1), that increases soybean resistance to P.sojae. However, the molecular basis of Gm DRR1 function remained largely uncharacterized. In the present study, analysis of Gm DRR1-RNAi, Gm DRR1-overexpressing, and CRISPR/Cas9-derived Gmdrr1 mutant lines revealed that Gm DRR1 expression significantly restricted P. sojae growth. Combining coimmunoprecipitation with liquid chromatography–tandem mass spectrometry revealed a Gm DRR1-interacting protein, Gm DRR2, which is homologous to Gm DRR1. An E-coniferyl alcohol coupling assay indicated that Gm DRR1 promotes the synthesis of(+)-pinoresinol, which helps to protect plants from P. sojae. The Gm NAC1(Glyma.05 G025500) transcription factor bound to the Gm DRR1 promoter both in vitro and in vivo to upregulate Gm DRR1 expression. Soybean resistance to P. sojae was increased by overexpression of Gm NAC1. Our findings suggest a novel signaling pathway involving a NAC transcription factor that mediates soybean resistance to P. sojae. Specifically, Gm NAC1 directly induces Gm DRR1 expression to increase resistance of soybean plants to P. sojae.

摘要： Soybean(Glycine max)is short-day(SD)plant.Flowering time is a key agronomic trait that determines the transition from vegetative to reproductive growth.The study on the expression and regulation mechanism of flowering time gene in soybean photoperiod control of flowering pathway is particularly theoretically significant for soybean genetic improvement.In this study,a dual-luciferase reporter gene system with the GmFT2a gene promoter as promoter sequence was constructed,and the method of Agrobacterium tumefaciens injection into tobacco leaves was selected to study the effects of long and short days on the activity of the GmFT2a gene promoter.The results of transient expression analysis showed that the GmFT2a promoter was strongly induced under the SD conditions in tobacco.Furthermore,analysis of the GmFT2a promoter sequence revealed several cis-acting elements,including G-Box,Box 4,GT1-motif and TCT-motif by PlantCARE search.It was speculated that these elements might promote the expression of GmFT2a gene in the SDs and played a role in promoting flowering.The results of this study provided a basis for a better understanding of the function of the GmFT2a gene and further exploration of the complex flowering mechanism of soybean.

摘要： The main defense response to Soybean mosaic virus(SMV)infection in soybean[Glycine max(L.)Merr.]is thought to be blockage of intercellular virus transport by callose deposition on plasmodesmata.But the specific regulatory mechanism remains largely unknown.In this study,we found that hydrogen peroxide(H_(2)O_(2))signal downstream of NO was associated with the regulation of callose accumulation.Abundant H_(2)O_(2)was produced on the cell membrane and cell wall in the incompatible combination of soybean cultivar Jidou 7 and SMV strain N3,whereas no obvious H_(2)O_(2)was observed in the compatible combination of Jidou 7 and strain SC-8.When H_(2)O_(2)production was inhibited,callose accumulation induced by SMV infection decreased to a level insufficient to restrict virus transport in the incompatible combination.The H_(2)O_(2)-associated transcriptome dynamics of soybean during SMV infection was investigated.Transcriptome and functional analysis using virus-induced gene silencing showed that Gm SEOB and Gm PAP27,two genes regulated by H_(2)O_(2),functioned in resistance by positively regulating the accumulation of callose in response to SMV infection.These results lay a foundation for further research on the signal transduction and molecular regulation of callose deposition during soybean resistance to SMV infection.

摘要： Soybean is one of the most important food crops worldwide.Like other legumes,soybean can form symbiotic relationships with Rhizobium species.Nitrogen fixation of soybean via its symbiosis with Rhizobium is pivotal for sustainable agriculture.Type Ⅲ effectors(T3Es)are essential regulators of the establishment of the symbiosis,and nodule number is a feature of nitrogen-affected nodulation.However,genes encoding T3Es at quantitative trait loci(QTLs)related to nodulation have rarely been identified.Chromosome segment substitution lines(CSSLs)have a common genetic background but only a few loci with heterogeneous genetic information;thus,they are suitable materials for identifying candidate genes at a target locus.In this study,a CSSL population was used to identify the QTLs related to nodule number in soybean.Single nucleotide polymorphism(SNP)markers and candidate genes within the QTLs interval were detected,and it was determined which genes showed differential expression between isolines.Four candidate genes(GmCDPK28,GmNAC1,GmbHLH,and GmERF5)linked to the SNPs were identified as being related to nodule traits and pivotal processes and pathways involved in symbiosis establishment.A candidate gene(GmERF5)encoding a transcription factor that may interact directly with the T3E NopAA was identified.The confirmed CSSLs with important segments and candidate genes identified in this study are valuable resources for further studies on the genetic network and T3Es involved in the signaling pathway that is essential for symbiosis establishment.