Southern

摘要： Psychology’s military use was discovered by ancient Chinese strategists in a very early time.In the Spring and Autumn period(770 B.C.-476 B.C.),psychological tactics were put into practice.After coming to the Northern and Southern Dynasties(420-589),psychological tactics had become the required course of strategists.Therefore,many generals of this period could use it adroitly.There were many classic examples which could help us analyze the psychological tactics of this era.The purpose of this paper is to introduce a few classic examples and summarize their characteristics.Then,we can better understand the psychological tactics of the Northern and Southern Dynasties.

摘要： 随着新课改内容的不断深入,在当前高中英语教学的课堂上,为了进一步深化教学效果,提升学生的学习认识,教师需要在教学方法的选择上引入更为多元化的思考,以此来完善整体的授课质量。为了达到这一目的,本文就新课改下高中英语课堂教学效果的优化方法展开探究,希望能够起到一些积极的参考作用。

摘要： Antarctica is the continent that surrounds the South Pole,the southernmost point at the bottom of the earth.Antarctica is a continent because it is a land covered by a thick layer of ice.It is a giant island that is surrounded by the Southern Ocean.The Southern Ocean is made up of the southern portions of the Atlantic.

摘要： The Arab-Chinese Cooperation and Development Association, a member of Silk Road NGO Cooperation Network, held a dialogue in the Kamel Jaber Cultural and Social Center of Nabatiyeh under the title ''Belt and Road Initiative and Development in South Lebanon''. More than 50 people including MP Yassine Jaber, the Chairman of the Foreign Affairs and Emigrants Committee in the Lebanese Parliament, Kassem Tofailli,Chairman of the Arab-Chinese Cooperation andDevelopment Association and President of theArab Chinese Cooperation and DevelopmentAssociation, members of the local municipalcouncil, and business people. Wang Kezhen, theChinese Ambassador to Lebanon, was invited toattend the meeting.

摘要： In this study, the roles of indigenous knowledge and farmers’ perception of natural resources management were analyzed. A total of seventy households were selected by simple random sampling with replacement method for collection of data. Formal interviews were conducted to the 5% plus of households in the study area in addition to the focus group discussions and qualitative field observations. It was expressed by 98.6% of the total respondents that “Sera” which is the traditional practice has being used to manage natural resources in the study area. In the “Sera” systems, individuals are excluded a punishment from any kind of social interaction in a response to their unlawful action in the community natural resources. It was indicated by 84.3% of respondents that deforestation and related problems are the major challenges including grazing and shortage of farm size for the management of the culturally protected forest in the study area. The majority of the respondents (77.1%) believed that soil fertility decline in the study area. Development of effective participatory forest management and encouragement and supporting the traditional method of natural resources management is required to ensure the preservation and protection of these areas essential to ecosystem service provision, provide high biodiversity value and cultural heritage, and maintain the sustainability of culturally protected forest.

摘要： Sand is a useful resource for the development of any society. As such, the issues related to its extraction are everywhere present in Benin, especially in the southern part of the country. For the purposes of eventually characterizing those issues, the present study is undertaken with a focus on inventorying estuarine and lagoonal ecosystems which are subjected to sand mining in southern Benin. The study area covers both the Eastern Complex and the Western Complex. The Eastern Complex includes the Porto-Novo Lagoon and the Ouémé River delta;while the Western Complex includes the Coastal Lagoon, the Aheme Lake along with its channels, and the Mono Basin. The method used in this study is a systematic inventorying of all known and documented sites as kept on record with relevant state offices in charge of the management of those resources. Then, the data were completed by combing the whole area for direct census observations and field interviews with stakeholders. All inventoried sites have been geo-referenced using a GARMIN GPS and their surface areas have been established. To date, 43 sand-mining sites have been identified, of which 29 are located in the Oueme Delta and in the Porto-Novo Lagoon, 8 are in the Coastal Lagoon, and 6 are in the Aheme Lake and its channels as well as in the Mono River basin. The areas are small in the Oueme Delta, where extraction is mostly manual, but get to be more than 89-ha wide in other places such as the Coastal Lagoon where the process is rather mechanized.

摘要： The Kyaikhto gold district is located within the Slate Belt and Mogok Metamorphic Belt of Southern Myanmar. The study area is covered by Carboniferous to Lower Permian metasedimentary rocks consisting of slate, phyllite, and schist of the Mergui Group, intruded by later igneous rocks. Four gold occurrences have been identified in the Kyaikhto district: the Kunzeik in the north, Zibyaung, and Thae Phyu Chaung in the center and Meyon in the south. Gold mineralization in the Kyaikhto district is associated with sheeted, stockwork, dissemination, and sulfide-bearing quartz veins. Ore minerals recognized include sphalerite, galena, chalcopyrite, molybdenite and pyrite with minor native gold and electrum. Two types of fluid inclusions were examined in the quartz samples of the Kunzeik and Zibyaung—Type A: aqueous carbonic fluid inclusions and Type B: aqueous fluid inclusions. At the Kunzeik, Type A fluid inclusions homogenize at temperatures from 296°C to 376°C with low salinities (1.6 - 4.6 wt% NaCl equivalent). The homogenization temperatures of Type B fluid inclusions in vein quartz range from 246°C to 312°C, with salinities of between 1.2 and 10.7 wt% NaCl equivalent. In the Zibyaung, the homogenization temperatures of Type A inclusions vary from 305°C to 378°C, with salinities from 4.6 to 9.6 wt% NaCl equivalent. The homogenization temperatures of Type B fluid inclusions mainly range from 242°C to 298°C, with salinities from 0.9 to 11.8 wt% NaCl equivalent. These characteristics of fluid inclusions are similar to those of orogenic gold mineralization systems.

摘要： This study presents the changes in lake areas in the Valley of Lakes, the Govi region, southern Mongolia. The recent changes in lake areas show decreases depending on vulnerability of lake basins and response of Govi landscape to the present climatic warming. During the recent 44 - 45 years (from 1970 to 2014 or 2015), modern lakes have encountered the present rapid increase in temperature, water evaporation and drying up that induced the reduction in lake areas in the Valley of Lakes. The finding of the reduction in lake areas is consistent with the trends on increasing in temperature since 1995 and fluctuating precipitation since 1975. Investigations with detailed chronology of lake sediment are needed from the lakes to review a more complete evolution of lake basins during the Late Quaternary paleoclimatic history in Mongolia and Central Asia.

摘要： 本发明涉及一种Southern印记杂交用恒压转膜夹，包括底部固定设置有压力计2的变压存液槽1，压力计2表面固定设置载物台3，载物台3上方设置有一个可上下运动的两端带孔的夹板4，带孔夹板4与变压存液槽1之间通过带有可拆卸旋转螺母5的加长螺杆6滑动连接，所述带螺母加长螺杆6固定设置在存液槽1的两侧，所述带孔夹板4下部中间位置固定连接带有螺栓7的液压伸缩杆8，所述液压伸缩杆8下端固定连接压板9，刻度尺10平行于螺杆6固定连接在存液槽1的后面。本发明通过设置在存液槽底部的压力计，能实现数字化准确确定作用在凝胶上的压力，使转膜过程压力保持稳定。另外，本发明还实现了恒压条件下快速方便地更换吸水纸，达到了转膜的最佳状态。

摘要： 本发明公开了一种基于Southern Blot的端粒长度检测方法，所述方法包含步骤：(a)从血液中提取人类基因组DNA，并配置酶切体系，反应条件为37°C水浴孵育16h，(b)将DNA酶切样本放入0.7％琼脂糖凝胶中电泳，反应条件为电压1.5V/cm，时间16h，反应过后修整琼脂糖凝胶，将其放置在干胶仪上，温度50°C，干燥1‑2h，涉及生物技术领域。该基于Southern Blot的端粒长度检测方法，避免了转膜效率对实验结果的影响以及克服了转膜对于DNA片段大小的限制，并且实验多步骤都在同一块凝胶上进行，使得实验操作连贯，实验结果稳定性高。

摘要： 沙门氏菌Southern印记杂交检测方法，其特征在于：包括以下步骤：沙门氏菌DNA获取，探针引物的制备，基因组DNA酶切，酶切产物的电泳，转膜，杂交和洗膜、信号检测。本发明的应用能有效的避免沙门氏菌检测过程中假阳性的出现，能准确判断样本的阴阳性，避免了传统方法中的耗时长，成功率低，设备要求高程序复杂等弊端。可广泛用于口岸中食品、公共卫生、牲畜沙门氏菌样品检测。

摘要： 鼠疫菌Southern印记杂交检测方法，其特征在于：包括以下步骤：鼠肝脏DNA的获取，探针引物的制备，基因组DNA酶切，酶切产物的电泳，转膜，杂交和洗膜、信号检测。本发明的应用能有效的避免鼠疫菌检测过程中假阳性的出现，能准确判断样本的阴阳性，避免传统方法中的分离培养、鼠疫噬菌体裂解试验和动物实验等的繁琐步骤，能够快速、准确的判断样本，可广泛用于口岸不同媒介生物监测点的鼠类样品检测。

摘要： 本发明涉及一种Southern印记杂交用恒压转膜夹，包括底部固定设置有压力计2的变压存液槽1，压力计2表面固定设置载物台3，载物台3上方设置有一个可上下运动的两端带孔的夹板4，带孔夹板4与变压存液槽1之间通过带有可拆卸旋转螺母5的加长螺杆6滑动连接，所述带螺母加长螺杆6固定设置在存液槽1的两侧，所述带孔夹板4下部中间位置固定连接带有螺栓7的液压伸缩杆8，所述液压伸缩杆8下端固定连接压板9，刻度尺10平行于螺杆6固定连接在存液槽1的后面。本发明通过设置在存液槽底部的压力计，能实现数字化准确确定作用在凝胶上的压力，使转膜过程压力保持稳定。另外，本发明还实现了恒压条件下快速方便地更换吸水纸，达到了转膜的最佳状态。

摘要： 本发明涉及一种用于Southern杂交分析的中华绒螯蟹血液DNA提取方法，特征是：采用以下提取步骤：将注射器吸取ACD抗凝剂，在中华绒螯蟹步足基部关节的软膜处插入，吸取血液，使ACD抗凝剂与血液充分混匀为混合液；经离心去除上清液，留血细胞沉淀；将血细胞沉淀悬浮于裂解液中混合为混合液；在混合液中加入Tris饱和酚，摇匀后离心分离；在上清液中加入氯仿，摇匀后离心分离，取上清液；在上清液中加入醋酸铵和无水乙醇，摇匀后离心分离，收集DNA；用70％乙醇洗后，风干，溶解。本发明抽提的DNA浓度较高，能满足Southern杂交方法。

摘要： 本发明公开了一种适用于脆性X综合征southern blot印迹杂交检测的DNA分子量标准，所述DNA分子量标准含有6条DNA片段，其长度分别为：2.8kb、2.9kb、3.3kb、5.2kb、5.3kb、和5.7kb，各个片段中均包括能与针对于FMR1基因的探针结合的片段，且均不含有EagI或EcoRI的酶切位点。本发明为southernblot印迹杂交技术对脆性X综合征进行诊断提供了一种特定的DNA分子量标准，实现对临床检测样本型别进行准确快速的判断。在‑20±5°C条件下保存3年仍然可以正常使用，不影响检测结果。且易制备、在贮存和使用过程中具有良好的稳定性和可重复性；无生物传染性。