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RNA干扰(RNAi)

RNA干扰(RNAi)的相关文献在2003年到2021年内共计66篇,主要集中在分子生物学、肿瘤学、基础医学 等领域,其中期刊论文65篇、专利文献57424篇;相关期刊55种,包括河北民族师范学院学报、广东海洋大学学报、昆虫学报等; RNA干扰(RNAi)的相关文献由271位作者贡献,包括魏太云、张伟、柴友荣等。

RNA干扰(RNAi)—发文量

期刊论文>

论文:65 占比:0.11%

专利文献>

论文:57424 占比:99.89%

总计:57489篇

RNA干扰(RNAi)—发文趋势图

RNA干扰(RNAi)

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  • 魏太云
  • 张伟
  • 柴友荣
  • 梁会娟
  • 田保明
  • 苗利娟
  • 谢联辉
  • 贾东升
  • 陈占宽
  • 陈红燕
  • 期刊论文
  • 专利文献

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    • 贾春松; 赵彦坡; 尚振华; 邢添瑛; 欧彤文
    • 摘要: 目的 探索通过RNA干扰(RNAi)降低膀胱TGF-β1表达治疗神经源性膀胱(NB)的效果.方法 将48只雌性大鼠均分为假手术组、T10脊髓横断组、LV-vector组和LV-shTGF-β1组.假手术组行T10脊髓横断假手术,其余3组行脊髓横断.术后即行膀胱壁注射,前两组为0.9%氯化钠溶液,LV-vector组为对照病毒,LV-shTGF-β1组为TGF-β1 RNAi慢病毒.术后第28天测压和称重膀胱,并将膀胱组织用HE和Masson染色,用免疫组化、RT-qPCR和Western blot检测逼尿肌TGF-β1的表达.结果 与假手术组相比,其余3组逼尿肌过度活动的次数和幅度显著增加(P<0.01),膀胱容量显著减小(P<0.05),膀胱质量和逼尿肌间质含量以及TGF-β1表达显著增加(P<0.05);T10脊髓横断组与LV-vector组相比,两者各项指标差异无统计学意义;LV-shTGF-β1组与T10脊髓横断组或LV-vector组相比,其逼尿肌过度活动的次数和幅度显著减少(P<0.01),膀胱容量显著增加(P<0.01),膀胱质量和逼尿肌间质含量以及TGF-β1表达显著减少(P<0.05).结论 RNAi降低膀胱TGF-β1表达能够改善脊髓损伤大鼠的逼尿肌过度活动和膀胱纤维化.
    • 刘廷利; 郭冬姝; 姚瑶; 张保龙
    • 摘要: 利用RNA干扰(RNA interference,RNAi)技术可以进行基因功能研究以及作物遗传改良,但常规构建RNAi载体方法费时费力.本研究报道了一种基于USER酶一步法快速构建RNAi发夹结构的载体2301/RNAi/OS及其应用方法,操作简便,省时省力.与已报道的利用酶切连接、Gateway兼容的同源重组或PCR直接连接等构建RNAi载体相比,该载体不需要酶切片段,片段扩增完成后即可与制备好的线性化载体进行反应,缩短操作流程和时间,提高成功率;该载体对插入片段的酶切位点和长度没有要求,理论上任何位置和长度的片段均可进入2301/RNAi/OS载体中,尤其是长片段RNAi的构建.本试验以本氏烟(Nicotiana benthamiana)PDS(Phytoene desaturase)基因为例,利用农杆菌介导的瞬时表达体系验证了该RNAi能够在植物体内有效地实现基因沉默.该载体以新霉素磷酸转移酶基因(NPTII)作为筛选标记基因,可以利用卡那霉素(Kanamycin)和G418(Geneticin)等抗生素筛选转基因阳性植株,适合单子叶和双子叶植物的遗传转化.
    • 刘金磊; 邓思平; 江东能; 陈华谱; 李广丽; 吴天利; 田昌绪; 朱春华
    • 摘要: [Objective] To investigate the functions of Estrogen-related receptor (ERR) in the reproductive process in female giant freshwater prawn (Macrobrachium rosenbergii).[Method] The expressions of ERR were knock-down by injecting double-stranded RNA (dsRNA). Ovary transcriptome sequencing was curried out from the ERR-dsRNA injected and un-injected control groups to screen differentially expressed genes related to reproduction and their expression pattern during ovarian development were detected by qPCR.[Result] The levels of ERR mRNA and ERR protein were significantly decreased after injecting ERR-dsRNA. Among the differentially expressed Unigenes obtained from ERR-dsRNA injected and control ovary transcriptomes, 9 Unigenes were enriched from reproduction GO term, 3 Unigenes were enriched to reproductive process GO term and 21 were identified to have been enriched to oocyte meiosis Pathway term. There are 9 Unigenes with large fold-changes in differential expression related to reproduction. Of these, 5 Unigenes were verified to be differential expressions between ERR-dsRNA interference and control groups by qPCR. Among the 5 differentially expressed Unigenes, 3 Unigenes were differentially expressed during ovarian development process. 2 of these Unigenes (CL7112.Contig4_All and Unigene4600_All) were associated with the oocyte meiosis and 1 unigene (CL4888.Contig2_All) was related to the synthesis and release of reproduction-associated hormones.[Conclusion] The function of ERR is likely to regulate ovarian development by regulating the oocyte meiosis as well as the synthesis and release of reproduction-associated hormones in M.rosenbergii.%[目的]研究雌激素相关受体(Estrogen-related receptor,ERR)在雌性罗氏沼虾生殖过程中的功能.[方法]通过注射双链RNA(Double-stranded RNA,dsRNA)干扰罗氏沼虾(Macrobrachium rosenbergii)ERR的表达,对干扰组和对照组的卵巢样本进行转录组测序,筛选与生殖相关的差异表达基因,并通过qPCR检测它们在卵巢发育过程中的表达.[结果]注射ERR-dsRNA后,卵巢中ERR mRNA和ERR蛋白的表达显著下降.ERR-dsRNA干扰组和对照组卵巢转录组测序获得的差异表达Unigene中,9条被富集到生殖GO条目,3条被富集到生殖过程GO条目,21条被富集到卵母细胞减数分裂Pathway条目.这些生殖相关的Unigene中差异倍数较大的有9条,经qPCR验证,5条在ERR干扰组和对照组间的表达差异有统计学意义.这5条Unigene中有3条在卵巢发育过程中的表达差异有统计学意义,其中2条(CL7112.Contig4_All和Unigene4600_All)与卵母细胞减数分裂有关,1条(CL4888.Contig2_All)与生殖相关激素的合成及释放有关.[结论]ERR可通过调控卵母细胞减数分裂和生殖相关激素的合成与释放调控罗氏沼虾卵巢发育.
    • 朱剑平
    • 摘要: 基因沉默是基因表达调控的一种重要方式,是生物体在基因调控水平上的一种自我保护机制.RNAi基因沉默技术,已成为分析人类基因组功能的有力工具,在病毒感染、肿瘤、移植免疫等疾病的治疗上有广阔的前景.本文简述了基因沉默技术的发展状况、研究进展和应用前景.
    • 毛颖波; 陈殿阳; 陈晓亚
    • 摘要: 非编码RNA参与了多种重要的生命进程,是当今研究的热点领域.在植物中,非编码RNA除了参与维持基因组的稳定,还在生长、发育、逆境胁迫反应等过程中发挥重要作用.RNA干扰(RNA interference,RNAi)是指由RNA触发的相应基因的表达抑制,是非编码RNA调控基因表达的一种重要的作用方式,自发现以来已逐渐发展为遗传分析、疾病治疗以及植物保护等方面的有效的新技术.文章重点介绍了微RNA (microRNA,miRNA)在植物抗虫防御中的生物学功能,siRNA对植物防御记忆的影响以及RNAi对植物防御信号途径的调节作用,同时阐述了RNAi在提高农作物抗虫性上的应用并展望了未来植物非编码RNA的研究方向.
    • 白福强; 谢纳; 夏晴; 董振杰; 麦艳娜; 刘文轩
    • 摘要: In this study RNA interference (RNAi) technology was applied to analyze the effect of RNAi silencing of tweleve important genes of wheat aphids by feeding aphids with double stranded RNA (dsRNA) of the target genes.The results showed that the mortality of wheat aphids significantly increased after RNAi silencing of water channel protein gene (Wsa) and ATP synthase gene (vAd1-78);whereas glucose dehydrogenase encoding gene (GlD-17) RNAi led to a significantly reduced mortality.Quantitative analysis of gene expression level during 5 days after RNAi showed that the expression level of gene Wsa and vAd1-78 was decreased by 74.8% and 44.6%,respectively.Whereas the expression level of gene GlD-17 was increased by up to 209%.Variance on mortality of wheat aphids was in accordance with gene expression level changes after gene silencing by RNAi.Among the twelve targeted genes,RNA interference of Wsa led to most significant gene silence effect,increasing mortality to 37.5% and down-regulating gene expression level to 74.8%.Thus water channel protein gene (Wsa)could be used as a candidate targeted gene of RNA interference for further development of wheat varieties of aphids resistance.%选用12个蚜虫生长发育的关键基因,利用喂食的方法将其双链RNA(dsRNA)引入麦长管蚜体内,以死亡率为评价指标,对基因RNA干扰(RNAi)沉默效应进行分析.结果表明,水通道蛋白基因(Wsa)、ATP合酶基因(vAdl-78)的RNA干涉能够显著提高蚜虫死亡率,而葡萄糖脱氢酶编码基因(GlD-17)的RNA干涉则使蚜虫死亡率明显降低.基因表达荧光定量PCR分析结果显示,喂食dsRNA后5d内,水通道蛋白基因(Wsa)和ATP合酶基因(vAdl-78)表达水平分别下降了74.8%和44.6%;而葡萄糖脱氢酶编码基因(GlD-17)表达水平则上调了209.0%,基因表达水平变化规律与蚜虫死亡率变化趋势一致.在所有供试基因中,水通道蛋白基因(Wsa)的RNA干涉沉默效应效果最突出,导致蚜虫死亡率提高到37.5%,基因表达量下调74.8%,可以作为利用RNAi技术培育抗蚜虫小麦新品种有效的靶基因.
    • 刘友梅; 薛敏峰; 史文琦; 黄薇; 袁斌
    • 摘要: According to the principle of RNA interference (RNAi),the expression of double stranded RNA (dsRNA) in vivo can efficiently silence gene expression in organism. A small RNA that forms pathogenic fungi and insect specific genes in the host will enter its body and produce host-induced gene silencing (HIGS). Crop diseases and insect pests pose a great threat to global food security,and the use of HIGS technology provides an effective way to improve crop disease and insect resis-tance. This paper summarized the application of HIGS technology on improving crop disease resistance and insect pests,and the possible transfer mode of silent signal between host and insect pest,and put forward the advantages and disadvantages of this technology.%根据RNA干扰(RNA interference,RNAi)原理,在生物体内表达双链RNA(dsRNA)可以高效沉默生物体内基因的表达.在寄主中形成病原真菌和昆虫特异基因的小RNA会进入其体内从而产生寄主诱导的基因沉默(Host-induced gene silencing,HIGS).病虫害对全球粮食安全构成极大的威胁,利用HIGS技术为提高农作物抗病虫性提供了有效的途径.综述了HIGS技术在提高农作物抗病虫性中的应用,总结了沉默信号在寄主与病虫间的可能的传递方式以及提出该技术存在的优缺点.
    • 曹忻; 邹云龙; 徐红伟; 杨具田
    • 摘要: [目的]明确RNA干扰(RNAi)对绵羊颗粒细胞体外培养过程中血管内皮生长因子(Vascular endothelial growth factor,VEGF)及其受体mRNA表达的影响,为开展VEGF在绵羊卵母细胞体外成熟和胚胎发育过程中信号通路的相关研究打下基础.[方法]采用电穿孔技术将针对VEGF两个受体Flt-1和KDR/Flk-1的两条有效双链小RNA导入绵羊颗粒细胞内,利用实时荧光定量PCR检测体外培养绵羊颗粒细胞各培养时间VEGF、Flt-1和KDR/Flk-1 mRNA表达水平的变化.设定筛选出的Flt-1和KDR/Flk-1有效干扰片段分别为试验组Ⅰ和试验组Ⅱ,两个干扰片段共同作用为试验组Ⅲ,无效的干扰片段为对照组.[结果]绵羊颗粒细胞体外培养过程中,VEGF mRNA在各时间段的相对表达量是Flt-1 mRNA相对表达量的102倍、KDR/Flk-1 mRNA相对表达量的103倍;培养第1 d时,试验组Ⅰ、试验组Ⅱ和试验组ⅢVEGF mRNA相对表达量分别为2.62×10-2、3.54×10-2和2.94×10-2,均极显著高于对照组(P<0.01,下同),3个试验组的VEGF mRNA表达量从第2 d开始降低,直到第6 d与对照组趋于一致.试验组ⅡFlt-1 mRNA相对表达量从培养第1 d的1.02×10-1逐渐降至第7 d的8.99×10-4,且一直极显著高于其他组;试验组Ⅰ和试验组Ⅲ在前3 d几乎无Flt-1 mRNA表达,随着培养时间的延长逐渐呈上升趋势,第8 d时各组趋于一致.试验组Ⅰ的KDR/Flk-1 mRNA相对表达量从第1 d开始极显著低于对照组;试验组Ⅱ和试验组Ⅲ在前3 d几乎无KDR/Flk-1 mRNA相对表达,随着培养时间的延长呈上升趋势,第9 d时各组趋于一致.[结论]两个有效干扰片段在绵羊颗粒细胞体外培养过程中起到很好的干扰作用,可改变VEGF、Flt-1及KDR mRNA的表达量,进而影响颗粒细胞相关生长信号的传输.%[Objective]The paper studied effects of RNA interference(RNAi) on mRNA expression of vascular en-dothelial growth factor(VEGF) and its receptors in the process of granule cells cultured in vitro, in order to lay a foundation for research in signaling pathway of VEGF during process of oocyte in vitro maturation and embryonic development.[Method]This research used electroporation to import two double chain small interfering RNA fragments which were de-signed for VEGF receptors Flt-1 and KDR/Flk-1 into ovine granule cells, and detected Flt-1 mRNA and KDR/Flk-1 mR-NA levels variation of ovine granule cells cultured in vitro at different times by real-time quantitative PCR. The researchers named the groups imported with Flt-1 interference fragment and KDR/Flk-1 interference fragment which were screened out in early experiment as treatment group Ⅰand treatment group Ⅱrespectively, and the group imported with both Flt-1 in-terference fragment and KDR/Flk-1 interference fragment as treatment group Ⅲ, and the group imported with interference fragment which had no RNAi effect as control group. [Result]Results indicated that, during the process of ovine granule cells cultured in vitro, mRNA expression level of VEGF was 102 times higher than that of Flt-1 and 103 times higher than that of KDR/Flk-1. On the 1st day, the VEGF mRNA expression levels of treatment groupⅠ, treatment groupⅡand treat-ment group Ⅲ were 2.62×10-2, 3.54×10-2 and 2.94×10-2 respectively, which were significantly higher than that of control group(P<0.01, the same below). The expression levels of the three treatment groups reduced since the 2nd day and tended to be similar to that of control group on the 6th day. During the culture process, Flt-1 mRNA level of treatment group Ⅱdropped from 1.02×10-1(on the 1st day) to 8.99×10-4(on the 7th day), and it had been significantly higher than that of other groups. Flt-1 mRNA expressions of both treatment groupⅠand treatment groupⅢcould be barely detected, then, as time passing by, they rose and tended to be similar to those of other groups on the 8th day. KDR/Flk-1 mRNA level of treatment groupⅠwas significantly lower than that of control group since the 1st day. KDR/Flk-1 mRNA in both treatment group Ⅱand treatment group Ⅲ could be barely detected from the 1st day to the 3rd day, and they rose with time passing by and tended to be similar to those of other groups on the 9th day. [Conclusion]Two pairs of effective interference fragments have good performance on interfering during the process of ovine granule cells in vitro culture, which results in change of mRNA expression levels of VEGF, Flt-1 and KDR mRNA, and affects transmission of growth signal concerning granule cells.
    • 王晓婷; 董慧; 王军; 全金梅; 张伟
    • 摘要: 针对单纯疱疹病毒1型(herpes simplex virus 1,HSV-1)的ICP27基因和其他疱疹病毒相关基因的高度保守区设计小干扰RNA (small interfering RNA,siRNA),研究其抑制病毒复制的效果.首先构建相应的小发夹RNA (small hairpin RNA,shRNA),然后通过病毒滴度测定、real-time PCR和细胞致病变效应(cytopathic effect,CPE)检测所设计的siRNA抑制病毒复制的能力.结果显示,所设计的shRNA-2(靶序列起始位置815)和shRNA-3(靶序列起始位置1 367)具有明显地抑制病毒复制的效果.尤其是shRNA-3,抑制病毒复制的效果更明显,在病毒滴度实验中,与阴性对照相比,其抑制倍数为81,同时可以下调ICP27基因的mRNA表达水平.实验结果表明shRNA-3能够显著抑制HSV-1病毒复制的能力,可以作为HSV-1感染性疾病的补充治疗手段,其对应的靶序列可以作为抗HSV-1新的靶标.%Small interfering RNAs (siRNAs) targeting ICP27 gene for herpes simplex virus 1 (HSV-1) and other highly conserved sequences for herpesviruses were designed and detected for their abilities to inhibit herpesvirus replication in vitro.Virus titer assay,real-time PCR and cytopathic effect (CPE) experiment were used to observe their inhibition effect in Vero cells challenged with HSV-1 by three small hairpin RNAs (shRNA-1,shRNA-2 and shRNA-3).The results suggested shRNA-2 (target sequence from 815 position) and shRNA-3 (target sequence from 1 367 position) could depress the replication of HSV-1.Especially,virus replication was obviously inhibited by shRNA-3 in the virus titer assay,and the inhibition ratio was 81 times comparing with Negative group.At the same time,the mRNA expression level of ICP27 gene could be decreased by shRNA-3.It indicated that shRNA-3 could effectively suppress the replication of HSV-1 and be used as a supplementary therapy for HSV-1 infectious diseases.The corresponding target sequence of shRNA-3 could therefore be used as a new target for anti-HSV-1 drugs.
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