摘要：
目的:探讨维生素B3 (Vit B3)促中性粒细胞生成的可能机制.方法:取21只健康雌性SD大鼠,随机分为3组(n=7):对照组(灌服0.9％ NaCl,2 mL/d,7d)、rhG-CSF组(皮下注射rhG-CSF,10 μg/d,7d)、Vit B3组(灌服Vit B3,500 mg/kg·d-1,7 d).检测干预前及干预后7d中性粒细胞计数(ANC),应用Bio-Plex悬液芯片系统检测粒细胞-巨噬细胞集落刺激因子(GM-CSF)、肿瘤坏死因子-α(TNF-α)、白介素-1β(IL-1β)、单核细胞趋化蛋白1(MCP-1)、巨噬细胞炎性蛋白-lα(MIP-lα)及肿瘤生长相关因子GRO/KC水平.结果:Vit B3组和rhG-CSF组ANC较干预前及对照组显著升高(P＜0.05),但两组比较差异无统计学意义(P＞0.05).3组干预前血清各细胞因子水平比较,差异无统计学意义(P＞0.05);干预7d后,Vit B3组TNF-α、IL-1β、MCP-1、GRO/KC明显升高,rhG-CSF组TNF-α显著升高,VitB3组IL-1β、MCP-1、GRO/KC明显高于rhG-CSF组,差异均有统计学意义(均P＜0.05).结论:Vit B3可诱导大鼠中性粒细胞生成,其机制可能与增加炎性因子TNF-α、IL-1β、MCP-1和GRO/KC分泌有关.%Objective:To investigate the effect and mechanism of Vitamin B3 (Vit B3)on granulopoiesis in normal rats.Methods:21 healthy female SD rats were randomly divided into three groups (n=7 per group):the control group in which the rats were given 9％ NaCl via gavage,the rhG-CSF group in which the rats were given 10 μg/d rhG-CSF via subcutaneous injection for 7 days,and the Vit B3 group in which the rats were given 500 mg/kg rhG-CSF for 7 days.The neutrophil granulocyte counts were analyzed before treatment and the 7th day after administration.The serum cytokines such as granulocyte macrophage colony stimulating factor (GM-CSF),tumor necrosis factor (TNF)-α,interleukin (IL)-1β,monocyte chemoattractant protein-1 (MCP-1),macrophage inflammatory protein-lα (MIP-lα)and tumor growth related factors GRO/KC levels were detected by Bio-Plex suspension array system.Results:The neutrophil counts increased significantly in Vit B3 and rhG-CSF groups (P＜0.05),but there was no significant difference between the rhG-CSF group and the Vit B3 group (P＞0.05).The levels of TNF-α,IL-1β,MCP-1 and GRO/KC in serum were significantly increased by Vit B3 administration (P＜0.05).The serum TNF-α level was increased by G-CSF treatment (P＜0.05).The levels of IL-1β,MCP-1 and GRO/KC were markedly higher in the Vit B3 group than those in the rhG-CSF group (P＜0.05).Conclusion:Vit B3 could result in increasing absolute neutrophil count in healthy rats,and the mechanism might be related to the elevation of serum inflammatory factors TNF-α,IL-1β,MCP-1 and GRO/KC levels.
