Protocol

摘要： Background:The emergence of Coronavirus SARS-CoV-2 evoked an unprecedented threat globally.Ever since the spread of this pandemic research and clinical trials have concentrated on the repurposing of already exciting FDA drugs to find a successful candidate to combat Covid-19.Objective:The objective of this study is to propose a therapeutic protocol that may have a potential solution to treat the severe infections associated with coronavirus.The clinical application of this protocol(Al-Akidi Therapeutic Protocol,A-TP)is highly recommended,as there are several scientific evidences that support this trusted protocol to be of great potential.Methods and Materials:This A-TP includes the use of one of the respiratory Fluoroquinolones(Levofloxacin or Moxifloxacin)in doses of 500 mg twice daily for 7-10 days,together with high doses of Vitamin D3(10000 IU/day)and Zinc(50 mg daily)for few weeks.This protocol is based on the previous antiviral activity of those Fluoroquinolones towards few viruses,the potent antibacterial activity on respiratory infections and high available concentrations in the lungs.It is also based on molecular docking of Levofloxacin and Moxifloxacin on various viral enzymes.Results:Molecular docking showed encouraging and very interesting docking scores and binding affinity of Levofloxacin and Moxifloxacin to certain viral enzymes,such as,RNA dependent RNA polymerase(RdRp),3-Cysteine-Like protease,Neuraminidase,Replicase polyproteins and Trans-Membrane Protease Serine 2 inhibitor(TMPRSS2).The highly expected clinical results of using this protocol are:reduce infection,control of temperature,improve breathing with less dependent on supplemented oxygen,and remarkable reduction of the pro-inflammatory cytokine storm,and hence,reduce hospitalization and mortality.Conclusion:Levofloxacin is highly recommended in managing the severe infections associated with Corona virus and has a remarkable reduction of pro-inflammatory cytokine storm as an immuomodulating agent.Levofloxacin is superior in this protocol over Moxifloxacin,due to its high excretion(≤83%)as unchanged through the kidneys,while Moxifloxacin is only 20%is excreted unchanged.It is an extra advantage of Levofloxacin to manage coronavirus in the kidneys.High doses of Vitamin D3 and Zinc are very useful to provide additional effective measures to combat Corona virus.Therefore,the use of this A-TP is highly and strongly recommended,as it serves the full requirements for excellent and potential therapy for the severe infections associated with Covid-19.

摘要： Background:Irritable bowel syndrome(IBS)is a common functional bowel disorder that can severely affect the quality of life of patients.Limited drugs have been reported for modern medical IBS treatment.The advantages of traditional Chinese medicine(TCM)treatment are gradually becoming prominent.Xi-Feng-Hua-Shi granules have been clinically used for diarrhea-predominant IBS(IBS-D)treatment for many years in TCM practice.Thus,this study aimed to further verify the effectiveness and safety of Xi-Feng-Hua-Shi(XFHS)granules in IBS-D treatment through a randomized,double-blind,placebo-controlled multi-center clinical trial and provide high-quality evidence for its effectiveness and safety in treatment,as well as provide a basis for clinical rational drug use and explore new clinical IBS-D treatment plans.Methods:A randomized,double-blind,placebo-controlled multi-center clinical trial will be performed in 23 hospitals.A total of 300 participants will be randomly divided into the experimental group(prescribed with XFHS granules)and the control group(prescribed with the placebo granules),with 150 participants in each group.The appearance,shape,color,and taste of the placebo granules are the same as those of XFHS granules.All participants will receive a 4-week treatment and a 6-month follow-up.The primary outcome is the overall clinical efficacy;the secondary outcomes are the IBS-Symptom Severity Score(IBS-SSS),TCM Syndrome Evaluation,and the IBS-Quality of Life(IBS-QoL)score,mental state assessment,and recurrence rate.Outcome measures(including primary and secondary outcome measures)are collected at baseline,as well as 2,4,16,and 28 weeks post-intervention.Discussion:This randomized,placebo-controlled,multi-center trial may provide high-quality evidence for the clinical XFHS granule efficacy in IBS-D treatment.Additionally,this study will conduct safety evaluations to provide a basis for clinical rational drug use.

摘要： Background:Diabetic peripheral neuropathy(DPN)has high incidence.At present,the treatment for DPN mainly focuses on controlling of blood sugar,nourishment of nerves and symptomatic treatment of pain which face some limitations,such as poor effects and adverse reactions of drugs and so on.Traditional Chinese Medicine(TCM)has certain curative effect in the prevention and treatment of DPN.Danggui Shaoyao powder,one of the famous classic prescriptions in TCM,has been often used clinically in the treatment of DPN in China,which without any relevant evidence-based medical research data.The protocol was designed to evaluate the effects of Danggui Shaoyao Powder in the treatment of DPN with qi deficiency and blood stasis pattern based on routine therapy.Methods:This protocol is designed as a three-arms parallel-group,outcome-assessor blinded RCT.303 Patients with DPN,from outpatient and inpatient departments of four clinical centers in China,will be randomly allocated into 3 groups(routine group,treatment group and control group).The course of treatment was designed as 1 month and followed up for 3 months.The TCSS scale,TCM syndrome scale,nerve conduction function,serum indexes will be tested to provide more clinical evidences for the effect of Danggui Shaoyao Powder on prevention and treatment of DPN.Finally,we will make statistics and analyse the data to draw a conclusion.Discussion:Danggui Shaoyao Powder is the ideal drug which is an agent acting on multiple targets related to DPN’s pathogenesis.We hope to find more clinical evidences for the effect of Danggui Shaoyao Powder on prevention and treatment of DPN through this RCT experiment.

摘要： Objective:A comprehensive and updated systematic review is needed to evaluate the effectiveness and safety of HuangQiXiXin decoction(HQXXD)for cough variant asthma(CVA).The aim of this systematic review protocol is to comprehensively assess the effectiveness and safety of HQXXD for CVA.Methods:An overall search for studies in main English and Chinese electronic databases from their inception to January 2021 will be performed.Randomized controlled trials regarding HQXXD for CVA will be included.We will use RevMan 5.3 software to perform statistical analysis and meta-analysis.Results:The findings of this systematic review will be disseminated through peer-reviewed publications.Conclusion:The systematic review will provide more evidence regarding the effectiveness and safety of HQXXD for CVA.

摘要： It is important to detect specific genes expressed in the guard cells,which control gas exchange and play key roles in response to drought and salt stresses.Due to the genetic transformation of Chinese cabbage(Brassica rapa)has not been well developed,in situ RT-PCR is a valuable option for detecting guard cell specific genes.We reported an optimized protocol of in situ RT-PCR by using an FAMA homologous gene Bra001929 in Brassica rapa.FAMA in Arabidopsis has been verified to be specially expressed in guard cells.We designed specific RT-PCR primers and optimized the protocol in terms of the(a)reverse transcription time,(b)blocking time,(c)antigen-antibody incubation time,and(d)washing temperature.Our approach provides a sensitive and effective in situ RT-PCR method for locating expression in the guard cells in Brassica rapa.Moreover,we proved the guard cell specific expression of Bra001929 in the epidermis indicating its’applicability as a marker gene for guard cells of Brassica rapa.

摘要： Background:Antibiotic-associated diarrhea is a clinical common symptom of antibiotics overuse and occurs in 5%-70%of adults.Xianglian pill has been traditionally considered as an efficient treatment of diarrhea and gastrointestinal diseases for thousands of years.However,no systematic review and meta-analyses have focused on its positive effects.Hence,this protocol for systematic review and meta-analysis was developed to evaluate the effect and clinical safety of Xianglian pill on treating antibiotic-associated diarrhea.Methods:All randomized controlled trials published in Chinese and English and assessed use of Xianglian pill for antibiotic-associated diarrhea will be included.Databases of PubMed,EMBASE,Cochrane Library,China National Knowledge Infrastructure,Chinese Biomedical Literature,Wanfang,and Chinese Science and Technology Periodical Database will be searched for randomized controlled trials from their inception until November 16,2020.Primary outcomes will be the incidence of diarrhea and adverse events,and secondary outcomes will be bowel movements and microbiome characteristics.Two authors will extract data and assess the risk of bias independently.Risk ratio will be used to evaluate the results,and meta-analyses will be conducted using STATA 15.0 software.The review aims to demonstrate the effectiveness of Xianglian pill in the prevention and treatment of antibiotic-associated diarrhea.

摘要： Dear editor,For multi-agent systems,consensus is the most fundamental problem,for which,vast studies have been reported,but a few achieved optimal protocols[1-9].As well known,optimal consensus is nontrivial due to the mismatching between the whole performance optimization(which usually leads to a centralized protocol)and the given distributed communication topology[1].

摘要： Background:Aphasia is one of the common complications of stroke.Speech and language therapy is a conventional treatment for aphasia following stroke.However,rehabilitation often remains unsatisfactory after speech and language therapy alone.Acupuncture may be effective for aphasia after stroke.We designed this systematic review to assess the efficacy and safety of acupuncture as an adjunct to speech and language therapy for aphasia following ischemic stroke.Methods:An extensive search will be performed in databases including Cochrane Central Register of Controlled Trials,PubMed,Web of Science,EMBASE,China National Knowledge Infrastructure,Sinomed,Wei Pu Date and Wan Fang Data.No limitation will be set in language,publication date or status of the study.The primary outcomes include scores of Western Aphasia Battery and Aphasia Quotient.The secondary outcomes include Scales of Clinical Rehabilitation Research Center Aphasia Examination,Communicative Abilities in Daily Living,Aphasia Battery of Chinese,Chinese Functional Communication Profile,adverse reactions,etc.Results:All of the data will be processed with Review Manager software.Cochrane‘risk of bias’tool will be used to assess the risk of bias.Conclusion:This study will contribute to the selection of appropriate therapies for aphasia after ischemic stroke and the development of clinical guidelines on this topic.

摘要： Objective:Lactation mastitis is a kind of disease with high incidence during puerperium.In the early stage,breast massage can effectively eliminate the symptoms.However,the value of breast massage is not fully known to the public,which restricts its promotion and popularization.One important reason is the absence of a multi-dimensional and overall evaluation of outcome.COS is strongly advocated internationally as the outcome in clinical research to promote the transformation of clinical evidence into practice.At present,there is no systematic review of the existing outcome.This protocol aims to provide a set of outcomes and outcome evaluation schemes for developing COS of breast massage in lactation mastitis early stage.Methods:We will search PubMed,Embase,Web of Science,CNKI,Wan-fang,VIP,SinoMed,Grey literature databases(master and doctoral thesis databases and conference literature databases of CNKI,Wan-fang and SinoMed),Clinical Trials Registry Platform portal,the Clinical Trials Registry,Australian,New Zealand Clinical Trials Registry,the Center Watch Registry,before March 1,2021.We will include randomized controlled trials and non-randomized trials,cohort studies,case series,case reports.We will assess the quality of outcome reporting according to the MOMENT,classify all outcomes using the OMERACT Filter 2.0 conceptual framework.Descriptive analysis will be performed for all categorical variables using frequency and mean.Result:This protocol aims to help guide the design of systematic review to summarize the existing outcomes,and comprehensively evaluate existing outcomes,classify outcomes and provide schemes of outcome evaluation for developing COS of breast massage in the early stage of lactation mastitis.Percentage and mean will be used to describe the extracted data.Conclusion:This is the first systematic review protocol to summarize outcomes,measurement instruments,assessment times,measurement frequencies,measurers about breast massage in the early stage of lactational mastitis.

摘要： Introduction:Constipation after stroke negatively affects the patients’quality of life.Moxibustion therapy may relieve the symptoms of constipation by moving intestinal Qi in accordance with traditional Chinese medicine theory.However,the effects of moxibustion therapy on constipation after stroke have not yet been systematically assessed.This review aims to assess the efficacy and safety of moxibustion therapy for constipation after stroke.Methods and analysis:We will search the following databases from inception to the present:Cochrane Central Register of Controlled Trials of The Cochrane Library,MEDLINE,EMBASE,Chinese Biomedical Database,China National Knowledge Infrastructure,VIP Database,and Wanfang Database.Randomized controlled clinical trials that assess the outcome of moxibustion for constipation after stroke will be included.Outcome measures will include mean weekly complete spontaneous bowel movements,the patient assessment of constipation,quality of life questionnaire,and others.Adverse events will assessed and reported for safety evaluation.Study selection and data extraction will be performed by two independent reviewers.Risk of bias assessment and data synthesis will be implemented using Review Manager software(version 5.3).Ethics and dissemination:This systematic review does not need ethical approval because no individual information will be involved.The protocol and full version of this systematic review will be published in a peer-reviewed journal.Updates will be conducted if there is enough new evidence that may cause any of the review conclusions to change.

摘要： In this paper,we propose new protocol called Efficient Dynamic Hybrid Routing protocol (EDHRP) for MANETs.By adopting ZRP,we can transfer message into very large zone with only a little increase of maintenance cost,but its network organization is constructed same as clustering.However,its zone center node has not responsibility to transfer messages through network as much like head node of clustering protocols.In this proposed protocol the nodes can be classified into 3 types:center node,border node,and normal node. By using three types zone,the proposed protocol can reach the radius of zone to 3-5 hops while maintenance cost is same as ZRP with the radius is only 2 hops.Our zone maintenance approach has shown that our protocol is much more efficient than ZRP and clustering protocols.In worst case of EDHRP,route finding process is same as ZRP with R=2 hops,but maintenance cost can be lower than as in ZRP. Also,each node has its own depends on their signal power,SO our protocol network zone is dynamic.

摘要： 本申请公开了一种应用于主节点的基于Protocol Buffer的镜像缓存方法，能够通过Protocol Buffer将全闪产品中批量化的小块IO聚合为一个大的IO，并向从节点发送相应的镜像请求，以实现镜像缓存。因此，能够大幅度减少主节点和从节点之间的IO交互次数和任务调度次数，显著提升存储系统性能。此外，本申请还提供了一种应用于主节点的基于Protocol Buffer的镜像缓存装置、一种应用于从节点的基于Protocol Buffer的镜像缓存方法及装置、全闪存储设备及全闪存储系统，其技术效果与上述方法的技术效果相对应。

摘要： 本申请公开了一种应用于主节点的基于Protocol Buffer的镜像缓存方法，能够通过Protocol Buffer将全闪产品中批量化的小块IO聚合为一个大的IO，并向从节点发送相应的镜像请求，以实现镜像缓存。因此，能够大幅度减少主节点和从节点之间的IO交互次数和任务调度次数，显著提升存储系统性能。此外，本申请还提供了一种应用于主节点的基于Protocol Buffer的镜像缓存装置、一种应用于从节点的基于Protocol Buffer的镜像缓存方法及装置、全闪存储设备及全闪存储系统，其技术效果与上述方法的技术效果相对应。