摘要：
Absract Objective:To establish a UPLC method for simultaneous determination of vicenin-2, verbascoside, isoverbascoside, crenatoside, pachypodol and pogostone in Pogostemonis Herba. Methods:Analysis was performed on a Waters BEH C18 column (100 mm×2.1 mm, 1.7μm). The mobile phase consisted of acetonitrile -0.1％ phosphoric acid in gradient mode. The flow rate was 0.4 mL·min-1. The column temperature was maintained at 30 °C and the detection wavelength was set at 309 nm. Results:Vicenin-2, verbascoside, isoverbascoside, crenatoside, pachypodol and pogostone showed a good linear relationship within the ranges of 2.18-109.00 μg·mL-1, 8.66-433.11μg·mL-1, 2.12-106.00 μg·mL-1, 1.81-90.40 μg·mL-1, 0.90-45.00 μg·mL-1 and 1.09-54.24 μg·mL-1, respectively (r2>0.999 7). The average recoveries of the six compounds were within 99.1％-100.7％. Results of precision, stability and reproducibility test were satisfactory. The contents of six components in ten batches of samples were 0.137-0.959 mg·g-1, 0.980-5.877 mg·g-1, 0.306-1.363 mg·g-1, 0.273-1.169 mg·g-1, 0.136-0.387 mg·g-1 and 0.292-1.619 mg·g-1, respectively. Conclusion:The established method can be used for the quality control of Pogostemonis Herba.%目的:建立同时测定广藿香中新西兰牡荆苷2、毛蕊花糖苷、异毛蕊花糖苷、列当苷、藿香黄酮醇和广藿香酮的超高效液相色谱(UPLC)分析方法.方法:采用Waters BEH C18 (100 mm×2.1 mm,1.7μm) 色谱柱;流动相为乙腈-0.1％磷酸溶液,梯度洗脱;流速0.4 mL·min-1;柱温30°C;检测波长309nm.结果:新西兰牡荆苷2、毛蕊花糖苷、异毛蕊花糖苷、列当苷、藿香黄酮醇、广藿香酮的线性范围分别为2.18～109.00、8.66～433.11、2.12～106.00、1.81～90.40、0.90～45.00、1.09～54.24μg·mL-1 (r2>0.999 7);平均回收率 (n=9) 为99.1％～100.7％,精密度、重复性、稳定性均符合有关规定;10批样品中6个成分的含量范围分别为0.137～0.959、0.980～5.877、0.306～1.363、0.273～1.169、0.136～0.387、0.292～1.619 mg·g-1.结论:本法经方法学验证,可用于广藿香质量控制.
