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同种移植

同种移植的相关文献在1989年到2019年内共计204篇,主要集中在外科学、基础医学、内科学 等领域,其中期刊论文203篇、会议论文1篇、专利文献9548篇;相关期刊128种,包括中国免疫学杂志、中华手外科杂志、中华外科杂志等; 相关会议1种,包括第一届《中华骨科杂志》论坛等;同种移植的相关文献由507位作者贡献,包括崔向东、何球藻、孟宏等。

同种移植—发文量

期刊论文>

论文:203 占比:2.08%

会议论文>

论文:1 占比:0.01%

专利文献>

论文:9548 占比:97.91%

总计:9752篇

同种移植—发文趋势图

同种移植

-研究学者

  • 崔向东
  • 何球藻
  • 孟宏
  • 郑树森
  • 陈奇鸣
  • 陈永良
  • 吕海建
  • 夏求明
  • 姚志发
  • 徐俊赐

同种移植

-相关会议

  • 期刊论文
  • 会议论文
  • 专利文献

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    • 林善; 陆赢; 蔡志明; 戴一凡; 牟丽莎
    • 摘要: 细胞、组织及器官移植是治疗器官功能衰竭、癌症等重大疾病直接有效的治疗策略.免疫排斥是同种及异种移植研究领域中难以彻底攻克的难题,因此对移植受体免疫排斥反应的实时监测尤为重要,针对同种及异种移植开发具有特异度高、早期灵敏度高、非侵入、快速等优点的生物标志物及其监测方法非常迫切.微小核糖核酸(miRNA)在免疫细胞的生成、发育以及免疫性功能中发挥重要的作用.本文对常规免疫排斥监测方法的局限性、miRNA的生物学特性以及miRNA在同种和异种移植免疫排斥中的应用进行总结,以探讨miRNA在同种和异种移植免疫排斥监测中的应用前景.
    • 胡定辉; 李鸿雁; 刘尊涛; 杜琎; 李航
    • 摘要: 目的 建立一种稳定可行的大鼠腹腔同种异体异位心脏移植模型.方法 选用40只SD大鼠作为供体和受体,随机分为A、B两组,在Ono创建的大鼠腹腔异位心脏移植模型的基础上,对麻醉方法、供心摘取、受体准备、吻合方法等进行了部分改进,术后统计手术时间及手术成功率.结果 正式实验20例存活14例,存活率70%.术中吻合口出血致死1例;术中移植心脏复跳后停跳l例;术后24h死亡4例,供心阻断时间为(30±5)min,手术时间为(50±5)min.结论 对经典的Ono大鼠腹腔异位心脏移植模型的手术步骤和操作流程进行优化和改进后,降低了建模的难度,缩短了手术时间,供体心脏保护好,手术成功率高.
    • 赵振林; 赵晨野; 谢燕兵; 杨波; 苏继荣
    • 摘要: Objective:To investigate the effect and mechanism of donor-Ag specific T cell vaccination on inducing specific immune tolerance of allogenic liver transplantation and the mechanism of immune privilege of liver transplantation .Methods:CBA mice were recipients,B6 mice were donors,T cell vaccination (TCV) were made from the attenuated spleen cells of CBA mice ,which were stimulated by Con A and were challenged with the spleen cells of B 6 mice.There are 3 groups in this experiment:Transplant control group:Orthotopic liver transplantation ( OLT) were performed with the recipients of CBA mice and donors of B 6 mice;Flt3-L treating group:OLT were performed with the recipients of CBA mice and donors of B 6 mice treated with Flt3-L;TCV group:OLT were performed with the recipients of CBA mice inoculated with TCV and donors of B 6 mice treated with Flt3-L.Median survival time (MST) of liver grafts was recorded, IL-4, IL-10 and IFN-γin peripheral blood were tested after transplantation in each group .One-way mixed lymphocyte reaction ( MLR) were carried out with effectors of spleen cells from CBA mice and stimulator of spleen cells from B 6 mice at the 5th day after transplantation.The apoptosis of liver graft infiltrating cells (GICs) were analyzed by flow cytometric analysis at the 5th day after transplantation.Results: Flt3-L treating donor activated allogenic acute rejecting reaction , TCV vaccinating recipient before and after transplantation significantly depressed the acute immune rejecting reaction mediated by Flt 3-L.The liver grafts were accepted by recipient without the presence of Flt 3-L.The cytokines test show that the serum value of IL-4 and IL-10 were increased in Transplant control group and TCV group ,but decreased in Flt3-L treating group.The value of IFN-γwas increased in Flt3-L treating.%目的:探讨供体抗原特异性T细胞疫苗诱导肝移植免疫耐受的作用及其机理,同时对肝移植“自动耐受”机制进行探讨。方法:以CBA小鼠作为受体,以B6小鼠作为供体,建立小鼠原位肝移植模型;制备T细胞疫苗:用B6小鼠的脾细胞免疫CBA小鼠,取CBA小鼠的脾淋巴细胞加以灭活,制备成T细胞疫苗(TCV)备用;实验分为3组:组1:单纯移植组,以CBA小鼠作为受体,以B6小鼠作为供体,做原位肝移植。组2:用Flt3-L治疗的B6小鼠作为供体,以CBA小鼠作为受体,做原位肝移植。组3:用Flt3-L治疗的B6小鼠作为供体,以TCV免疫过的CBA小鼠作为受体,进行原位肝移植。组间比较移植物存活时间(MST);测定肝移植小鼠血清中的IL-10、IL-4和IFN-γ水平;组内比较移植前后单向混合淋巴细胞反应(MLR);分离移植物浸润细胞( GICs)组间比较GICs凋亡率。结果:Flt3-L诱导了同种移植急性排斥反应,应用TCV于移植前和移植后可以显著抑制Flt3-L诱导的同种肝移植排斥反应。在没有Flt3-L刺激作用的情况下移植排斥反应非常轻微。肝移植小鼠血清中的IL-10、IL-4和IFN-γ水平检测发现,Flt3-L治疗组IFN-γ水平显著升高,IL-10、IL-4水平降低;TCV免疫组IL-10、IL-4水平显著升高,IFN-γ水平显著下降;单纯移植自动耐受组与TCV免疫组变化趋势一致。单向混合淋巴细胞反应( MLR)显示:在单纯移植组,淋巴细胞增殖效应不明显,CPM为3318±1190;在Flt3-L治疗组,效应细胞增殖显著增强,CPM 为9790±1369,在TCV免疫组,TCV免疫加Flt3-L治疗,淋巴细胞增殖效应被显著抑制,CPM为4017±880。分离肝脏移植物内浸润细胞凋亡,TCV免疫组引起TIL凋亡率为(54.20±3.12)%,有效抑制了TIL引起的免疫排斥反应,诱导了免疫耐受。而在Flt3-L治疗组,TIL凋亡率为(3.34±2.49)%。结论:小鼠同种肝移植存在“自动耐受”现象;这种“自动耐受”现象的免疫平衡可以被Flt3-L打破,从而诱发急性排斥反应。针对排斥效应T细胞的特异性T细胞疫苗可以显著抑制Flt3-L诱导的同种肝移植排斥反应。
    • 王志刚; 胡启翔; 刘锵; 郝朝辉
    • 摘要: 目的观察复合皮移植术在大面积烧伤瘢痕整形中的临床应用效果。方法 2010年3月至2013年3月,应用复合皮移植术治疗8例大面积烧伤后瘢痕患者,均为男性,年龄23~47岁,共13个手术部位,其中腘窝部5例,膝部3例,小腿部2例,肘关节2例,大腿部1例。切除瘢痕组织后创面为4.0cm×7.0cm^15.0cm×18.0cm,采用一步法将异体脱细胞真皮基质与大张自体刃厚皮片复合移植修复创面。结果 8例患者随访3~22个月,复合皮成活率92%,愈合后外观平整,无排斥反应,关节功能良好。结论复合皮是修复大面积烧伤后瘢痕的理想材料。
    • 荆辉; 常青
    • 摘要: 背景:目前同种瓣已在临床广泛应用,但移植后远期易发生衰败和钙化。目的:探究同种带瓣主动脉经去内皮和去细胞处理后,生物学特性和免疫原性的变化,寻找更好的同种瓣去细胞处理方法。方法:取家兔带瓣主动脉根部,随机分为新鲜组、去内皮组、去全部细胞组。分别采用苏木精-伊红、Masson、VG染色进行组织形态学观察;生物学特性评价组织厚度、抗张强度、断裂拉伸率、热皱缩温度;通过免疫组织化学染色观察及原子火焰吸收法测定移植物钙含量变化进行免疫原性评价。结果与结论:组织形态学观察发现去内皮和去细胞方案均达到了对应的去细胞目的。与新鲜组相比,去内皮组抗张力性能变化不明显(P〉0.05),去细胞组显著下降(P〈0.01)。异体移植实验后苏木精-伊红染色观察发现:新鲜组炎性反应最为显著,组织中有大量炎细胞浸润;与新鲜组相比,去内皮组炎性反应明显减轻,炎性细胞数目明显减少,去细胞组炎性反应最轻,仅组织边缘有炎性细胞浸润。免疫组织化学发现:去内皮组和去细胞组CD152表达水平明显高于新鲜组(P 〈0.05)。钙含量分析发现:去内皮组和去细胞组钙盐含量较新鲜组显著下降(P〈0.05)。提示去内皮和去细胞同种带瓣主动脉的免疫原性均显著下降,而去内皮处理能更好地保留同种瓣的抗张性能。
    • 荆辉; 常青
    • 摘要: BACKGROUND:Aortic root homograft has been widely used, but it stil has a high incidence of calcification and degeneration after operation. OBJECTIVE:To investigate the alteration of biological and immunological properties of rabbit aortic root homograft treated with de-endothelialization and decellularization methods, and to find a better decellularization method. METHODS:Aortic root homografts obtained from rabbits were randomly divided into fresh group, de-endothelialization group, and decellularization group. The morphology of homografts was evaluated by hematoxylin-eosin, Masson and VG stainings. The biological properties such as thickness, tensile strength, broken extension rate, and heat shrinkage temperature were detected. The immunological features (inflammatory cellinfiltration, calcium content and CD152 expression) were determined with immunohistochemistry and flame atomic absorption spectrometry. RESULTS AND CONCLUSION:As demonstrated in morphological observation, protocols of de-endothelialization and decellularization methods we used attained our desired aim of decellularization perfectly. Tensile strength and broken extension rate of decellularization group were decreased significantly compared with fresh group (P0.05). Hematoxylin-eosin staining showed that, inflammatory reaction was the most apparent in fresh group, showing a large amount of inflammatory cells infiltration;compared with fresh group, inflammatory reaction was significantly attenuated in de-endothelialization group, the number of inflammatory cells was significantly decreased. The inflammatory reaction was the lowest in the decellularization group, only inflammatory cells infiltrated the tissue edge. Immunohistochemistry showed that, CD152 expression was significantly increased in de-endothelialization and decellularization groups compared with fresh group (P0.05),去细胞组显著下降(P<0.01)。异体移植实验后苏木精-伊红染色观察发现:新鲜组炎性反应最为显著,组织中有大量炎细胞浸润;与新鲜组相比,去内皮组炎性反应明显减轻,炎性细胞数目明显减少,去细胞组炎性反应最轻,仅组织边缘有炎性细胞浸润。免疫组织化学发现:去内皮组和去细胞组CD152表达水平明显高于新鲜组(P <0.05)。钙含量分析发现:去内皮组和去细胞组钙盐含量较新鲜组显著下降(P<0.05)。提示去内皮和去细胞同种带瓣主动脉的免疫原性均显著下降,而去内皮处理能更好地保留同种瓣的抗张性能。
    • 张艳; 陈栋; 李明; 李永海; 刘斌; 陈刚; 陈实
    • 摘要: Objective:To investigate the allograft immune hyporesponsiveness induced by complement regulatory protein Crry as well as dendritic cells( DCs) involved. Methods:DCs were isolated and cultured from bone marrow of BALB/c mice. The experiment was divided into 2 groups, ①LPS-stimulated group, DCs were cultured and stimulated by LPS for 48 h; ② Crry-treated group, DCs were cultured and anti-Crry antibody were added to culture medium with the concentration of 5 μg/ml, DCs were stimulated by LPS for 48 h. The expression of CD86, CD40, MHC Ⅱ in DCs were examined by flow cytometry. Production of interferon-γ(IFN-γ) , interleukin-10(IL-10) and interleukin-12(IL-12) was detected in the supernants of different groups by ELSIA; complement C3,C5, C3a and C5a in the supernants of different groups were detected by ELISA; Allo-MLR was examined by MTT colorimetry with DCs from BALB/c mice as the stimulators and CD4+ T cells of C57BL/6 mice as responders. Results: Compared with LPS-stimulated group, CD86 and MHC Ⅱ expression in DCs , C3a, C5a,IFN-γ and IL-12 in the supernants were significantly decreased(P <0. 05) , IL-10 in the supernants was significantly increased (P < 0. 05) , Crry-stimulated DCs significantly inhibited proliferation in mixed lymphocyte culture (P<0.05). Conclusion: Complement regulatory protein Crry modulated costimulatory molecular expression, cytokines production, and complement production in DCs, which induces allograft immune hyporesponsiveness.%目的:探讨补体调节蛋白Crry对树突状细胞(DC)的调节作用及诱导同种移植免疫低反应性的机制.方法:分离BALB/c小鼠的骨髓来源树突状细胞,试验分为2组:①脂多糖(LPS)刺激组,即培养结束前加入外源性脂多糖(1 mg/L)刺激24小时;②Crry处理组,加入抗小鼠Crry抗体5μg/ml,结束前加入外源性脂多糖(1 mg/L)刺激24小时,流式细胞仪检测树突状细胞表面分子CD86 、CD40、MHCⅡ的表达,ELISA法检测其细胞上清液中IFN-γ、IL-10和IL-12的水平,ELISA法检测细胞上清液中C3、C5、C3a和C5a的含量,并以BALB/c小鼠的DC作为刺激细胞,以C57 BL/6小鼠的淋巴细胞作为反应细胞进行同种混合淋巴细胞培养,MTT法检测淋巴细胞增殖活性.结果:经LPS刺激后,培养的DC表面共刺激分子CD40、CD86和MHCⅡ分子的表达显著增加(P <0.05);Crry处理组DC表面共刺激分子CD86和MHCⅡ分子的表达较LPS刺激组显著降低(P<0.05),而CD4O的表达无显著性差异;Crry处理组培养上清液中C3和C5的含量无明显变化,但C3a、C5a的含量较LPS刺激组显著减少(P<0.05);培养DC上清液中IFN-γ和IL-12的含量较LPS刺激组显著降低(P<0.05),IL-10的含量较LPS刺激组显著增加(P<0.05);培养的DC加入Crry抗体后,其淋巴细胞增殖活性显著降低(P<0.05).结论:补体调节蛋白Crry可以对DC具有重要的调控作用,可以影响其共刺激分子的表达、补体的生成以及细胞因子的合成等,从而诱导同种移植免疫低反应性,丰富先天免疫对获得性免疫的调节作用.
    • 罗程; 郑宝石; 何巍; 冯旭; 雷宾峰; 彭俊
    • 摘要: 目的 探讨环孢素预处理对同种异体带瓣主动脉移植后钙含量的改变及其意义.方法 4周龄的白兔50只(取带瓣动脉)为供体,体重0.30~0.35 kg;8周龄白兔50只,体重3.00~3.50 kg,随机分2组为受体,25只(环孢素预处理移植带瓣主动脉)实验组,25只(环孢素预不处理移植带瓣主动脉)对照组,分别于术后2、4、8周取出血管测定CD25和CD40的表达,原子吸收光谱法测定组织钙含量.结果 ①对照组CD25和CD40表达水平的高峰在移植后2周,此后逐渐回落,实验组在移植后2~4周的表达明显低于对照组(P<0.01);②实验组在2、4、8周3个不同时间点的钙含量明显低于对照组(P<0.01).结论 移植后使用环孢素抑制了早期的免疫排斥反应,降低了同种带瓣血管的钙化程度.
    • 齐丙迪; 孟宝玺; 杨阳; 刘蓓; 李翅翅; 夏炜; 郭树忠; 张晨
    • 摘要: Objective To study the immuno-tolerance mechanism of the third-party bone marrowderived mesenchymal stem cells ( BMSCs) in the allogeneic transplantation. Methods Forty female C57BL/ 6 mice and forty male BALB/C mice were respectively used as donors and recipients in skin allogenic graft model. Forty male BALB/C mice were divided randomly into 4 groups: blank control group, CP group, BMSCs group , CP + BMSCs group , with 10 mice in each group. Before skin graft, high-dose abdominal injection of cyclophosphamide ( 200 mg/kg,2 d,q. d. ) was performed in recipient mice in CP and CP + BMSCs groups. On the transplantation day, a bonus of 2 x 106 BMSCs from the SD rat (SD-BMSCs) were injected through the tail vein in the BMSCs and CP + BMSCs groups. The observation and HE staining of skin grafts were used. The expressions of CD29, CD34, CD45 and CD90 of cells were analyzed by using flow cytometry in order to identify BMSCs. The CD4+ , CD25+ , Foxp3+ and Treg cells of spleen were detected by flow cytometry. Cytokine in peripheral blood of recipient mice were measured by ELISA,including TGF-β, IL-10 and IFN-γ. T cells were co-cultured with 60 Co-irradiated bone marrow MSCs from different individuals. The proliferative activity of T cells were evaluated with MTT assay. Results The skin graft survival time was significantly prolonged in the CP + BMSCs group, as compared with that in the blank control group, the CP group, the BMSCs group, respectively. Cells cultured by whole bone marrow adherent cultivation showed CD29+ (99.7% ) ,CD44+ (96.7% ) ,CD34 (1.6% ) ,CD45( 1. 3% ). Compared with the control group and CP group, the ratio of the CD4+ ,CD25+ ,Foxp3+ and Treg cells significantly increased in the SD-BMSCs group and CP + BMSCs group (P < 0. 05). Analysis of peripheral blood by ELISA showed significant high level of TGF-β, IL-10 and low level of IFN-γ in BMSCs group and CP group, compared with that in control group. When co-cultured with BMSCs from different individuals, T- lymphocytes proliferation decreased apparently in SD-BMSCs group and C57-BMSCs group (P < 0. 05) , but there was no significant difference between SD-BMSCs group and C57-BMSCs group ( P > 0. 05 ). Conclusions The immunotolerance mechanism of the third-party bone marrow-derived mesenchymal stem cells in the allogeneic transplantation might be associated with its effect on the proliferation of Treg cells and increasing expression of TGF-β and IL-10, decreasing expression of IFN-γ.%目的 初步研究第三方骨髓间充质干细胞(bone marrow-derived mesenchymal stem cells,BMSCs)诱导同种异体移植受体免疫耐受的作用机制.方法 40只雌性C57BL/6小鼠作为供体,40只雄性BALB/C小鼠作为受体,建立稳定的同种异体皮肤移植模型,BMSCs取自SD大鼠骨髓.将40只BALB/C小鼠随机分为4组,每组10只.①空白对照组:只进行皮肤移植,未给予其他治疗;②环磷酰胺组(CP组):大剂量环磷酰胺(cyclophosphamide,CP)腹腔注射,200 mg/kg,连用2 d(q.d.);③单纯给予SD-BMSCs移植组(SD-BMSCs组):移植当天自受体小鼠尾静脉输注2×106个SD-BMSCs;④细胞药物联合应用组(CP+SD-BMSCs组):大剂量CP腹腔注射,200 mg/kg,连用2 d(q.d.),并于移植当天自受体小鼠尾静脉输注2×106个SD-BMSCs.检测指标包括:移植皮片存活情况;SD大鼠BMSCs表面抗原CD29、CD34、CD45和CD90鉴定;流式细胞仪检测受体脾脏调节性T细胞(CD4+、CD25+、Foxp3+、Treg细胞)的比例;ELISA检测受体外周血TGF-β、IL-10、IFN-γ的含量;异基因T淋巴细胞与经60Co照射的不同来源BMSCs共培养后,MTT法测定异基因T淋巴细胞增殖的情况.结果 CP+SD-BMSCs组皮肤移植物存活时间为(15.7 ±1.4)d,空白对照组为(6.1±1.1)d,CP组为(12.3±1.5)d,SD-BMSCs组为(12.6±1.8)d,CP+SD-BMSCs组皮肤移植物存活时间明显比后3组延长(P<0.05).全骨髓贴壁培养的BMSCs表面抗原鉴定:CD29+、CD44+分别为99.7%和96.7%,CD34-、CD45-分别为1.6%和1.3%.流式细胞仪检测Treg含量SD-BMSCs组和CP+SD-BMSCs组明显高于空白对照组和CP组(P<0.05);ELISA检测受体外局血SD-BMSCs组和CP组TGF-β和IL-10明显高于空白对照组,SD-BMSCs和CP组IFN-γ则明显低于空白对照组(P<0.05);共培养结果显示:来源于C57小鼠和SD大鼠的BMSCs可以明显抑制T淋巴细胞的增殖反应(P<0.05),而上述两组组间比较差异则无统计学意义(P>0.05).结论 第三方BMSCs诱导同种异体移植免疫耐受作用可能与诱导受体Treg细胞增殖和促进免疫耐受因子的表达,抑制免疫排斥因子的表达有关.
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