摘要:
本试验旨在研究不同来源饲粮纤维对福建黄兔盲肠发酵参数及菌群数量的影响.选取200只体重相近、健康的 (35±2) 日龄断奶福建黄兔, 采用单因子完全随机区组试验设计, 随机分为4组, 分别饲喂以苜蓿干草、甜菜渣、燕麦草、大麦糠为粗饲料原料, 以25%的添加比例配制成的4种等能等粗蛋白质的配合饲粮, 即高可溶性纤维 (HSDF) 饲粮 (包括2种纤维源, 分别为苜蓿干草和甜菜渣) 、高不溶性纤维 (HIDF) 饲粮 (纤维源为大麦糠) 和对照 (CK) 饲粮 (纤维源为燕麦草) , 每组5个重复, 每个重复10只.预试期为7 d, 正试期为53 d.在试验期末, 测定盲肠内容物pH、氨态氮 (NH3-N) 和挥发性脂肪酸 (VFA) 含量及盲肠菌群数量.结果表明:1) HSDF组的pH、NH3-N含量极显著低于HIDF组 (P<0.01) ;同时HSDF组的乙酸、丙酸、丁酸和总挥发性脂肪酸 (TVFA) 含量极显著高于对照组和HIDF组 (P<0.01) .2) HSDF组的乳酸杆菌数量极显著高于对照组和HIDF组 (P<0.01) , 其中甜菜渣组的大肠杆菌数量极显著高于苜蓿干草组 (P<0.01) .HSDF组的黄色瘤胃球菌数量最高, 极显著高于HIDF组和对照组 (P<0.01) .由此可见, HSDF (苜蓿干草、甜菜渣) 对福建黄兔盲肠发酵及菌群有较好的效果.%The purpose of this study was to investigate the effects of dietary fibers with different sources on cecal fermentation parameters and microflora amount of Fujian yellow rabbits.A total of 200 healthy Fujian yellow rabbits with similar body weight and at age of (35±2) days were randomly divided into 4 groups with5 replicates per group and 10 rabbits per replicate using a single-factor complete randomized block design.Rabbits fed 4 diets with equal energy and crude protein, which used alfalfa hay, beet slag, oat hay and barley bran as fiber sources, respectively, and added in the proportion of 25%.The 4 diets were high soluble fiber (HSDF) diet (including 2 fiber sources, alfalfa hay and beet slag) , high insoluble fiber (HIDF) diet (barley bran as fiber source) and control (CK) diet (oat hay as fiber source) , respectively.The preliminary trial period lasted for 7 d, and formal trial period lasted for 53 days.At the end of the trial period, pH, ammonium nitrogen (NH3-N) and volatile acid (VFA) contents and microflora amount were measured in the caecal contents.The results showed as follows:1) the pH and NH3-N content in HSDF group were significantly lower than those in HIDF group (P<0.01) .At the same time, the contents of acetic acid, propionic acid, butyric acid and total volatile acid (TVFA) in HSDF group were significantly higher than those in control group and HIDF group (P<0.01) .2) The number of Escherichia coli and Lactobacillus in HSDF group was significantly higher than that in control group and HIDF group (P<0.01) , and the Escherichia coli amount in beet slag group was significantly higher than that in the alfalfa hay group (P<0.01) .The number of Ruminococus flavefaciens was the highest in HIDF group, which was significantly higher than that in HIDF group and control group (P<0.01) .It can be seen that HSDF (alfalfa hay and beet slag) has better effects on cecum fermentation and bacteria for Fujian yellow rabbits.