A - Shiga Toxin A Subunit Effector Polypeptides Shiga Toxin Effector Scaffolds and Cell-Targeting Molecules for Site-Specific Conjugation

摘要

The present invention relates molecules to site-specific locations (and sometimes unique amino acid residues of a molecule) for binding to other molecules while maintaining Shiga toxin functions such as, for example, efficient intracellular routing and/or potent cytotoxicity. Shiga toxin A subunit-derived polypeptides, scaffolds and cell-targeting molecules comprising amino acid substitutions that provide The present invention also relates to cell-targeting molecules, and/or components thereof, comprising site-specific sites for not binding to other molecules, such as, for example, cell-targeting molecules or agents that alter the properties of the cargo for delivery. provide them Certain molecules comprising a polypeptide of the invention exhibit reduced immunogenicity and/or are well tolerated in mammals. The cell-targeting molecules of the invention and compositions thereof have use as diagnostic and/or therapeutic molecules, e.g., for the selective delivery of cargo to target-expressing cells, and for the treatment of various diseases, disorders and conditions, This includes genetic disorders, genetic predisposition, infection, cancer, tumors, growth abnormalities, and/or immune disorders.