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Method for determining the possibility of contamination by type II cell swelling lethal toxin-producing bacteria
Method for determining the possibility of contamination by type II cell swelling lethal toxin-producing bacteria
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机译:II型细胞肿胀致死毒素产生菌污染可能性的测定方法
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摘要
PROBLEM TO BE SOLVED: To efficiently detect a type II cell swelling lethal toxin-producing bacterium. SOLUTION: This is a method for determining the possibility of contamination by Escherichia Alberti, a type II cell swelling lethal toxin-producing bacterium, and amplifies the target gene fragment in the sample by contacting it with the sample to be determined or DNA extracted from the sample. In the step of amplifying the target gene fragment, preferably any of the primers having the base sequence represented by SEQ ID NOs: 48 to 58 and the primer having the base sequence shown by SEQ ID NOs: 59 to 67. Use a primer set consisting of either. [Selection diagram] Fig. 1
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机译:需要解决的问题:有效检测II型细胞肿胀致死毒素产生菌。解决方案:这是一种用于确定埃希氏菌(一种II型细胞肿胀致死毒素产生菌)污染可能性的方法,并通过与待测样本或从样本中提取的DNA接触来放大样本中的目标基因片段。在扩增目标基因片段的步骤中,优选具有由SEQ ID NOs:48至58表示的碱基序列的任何引物和具有由SEQ ID NOs:59至67显示的碱基序列的引物。使用一套底漆,由以下两种材料组成:。[选择图]图1
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