Protein sequence optimization method using amino acid co-evolutionary information

摘要

The present invention relates to a method for designing a protein-optimized mutant sequence using amino acid co-evolution information, and specifically, a novel protein with improved expression level, water solubility, stability and functionality while maintaining the original function of a protein using amino acid co-evolution information and protein tertiary structure information. It relates to a method for searching for a protein having a mutant sequence.