Single molecule determination and quantitative analysis of DNA nanotechnology in micropores

摘要

The invention relates to a method and to a DNA nanostructure for detecting a target structure. The invention relates in particular to a DNA nanostructure, which, by skillful selection of the shape of the DNA nanostructure and the placement of marker molecules fastened thereon, ensures a preferably linear dependence on the number of marker molecules and measurement signal, independent of the local arrangement of a plurality of such DNA nanostructures. The invention further relates to using said DNA nanostructures and other nano reporters, preferably in combination with adaptors, to quantify, specifically binding to target molecules in a method for the preferably simultaneous quantification of a plurality of target molecules, by means of a multiplex method. The method relates in particular to a single cell analysis.