Improved in situ hybridization reaction using short-chain hairpin DNA

摘要

PROBLEM TO BE SOLVED: To clearly fluoresce a target substance in a living body in an In situ HCR method capable of visualizing and detecting RNA in cells or tissues. In an in situ hybridization method, a sample containing a target analyte contains a split-initiator probe pair having a sequence complementary to the sequence of the target analyte and a fluorescently labeled H1 sequence or complementary to each other. By using a short chain hairpin pair having an H2 sequence, a method for identifying a target analyte in a living tissue is provided. [Selection diagram] Fig. 1