Engineered dysbiosis-sensing probiotics for the management of Clostridium difficile infections and recurrent infections

摘要

The present invention relates to C. in the human gastrointestinal tract. A method for metabolic engineering of bacterial cells to produce bile salt hydrolases to inhibit germination and colonization of difficile endospores. The bile salt hydrolase is operably linked to a sialic acid-inducible promoter, pNanA, of which pNanA is in turn controlled by a repressor of nanR. Recombinant bacteria expressing a bile salt hydrolase are C. It may be a probiotic strain used for the prevention or treatment of difficile infection.