PCR BASED DIAGNOSTIC KIT, COMPOSITIONS AND METHODS FOR AMPLIFICATION AND DETECTION OF SARS-COV-2

摘要

The present application is directed to a method for detecting presence or absence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in a sample. The method first involves contacting the sample with a primary oligonucleotide primer set. The primary oligonucleotide primer set comprises: (i) a first oligonucleotide primer comprising a nucleotide sequence complementary to a first portion of the SARS-CoV-2 transmembrane domain 2 gene, and (ii) a second oligonucleotide primer comprising a nucleotide sequence complementary to an extension product formed from the first oligonucleotide primer of the primary oligonucleotide primer set. The method can further involve contacting the sample with a secondary oligonucleotide primer set, where the secondary primer set comprises (i) a first oligonucleotide primer comprising a nucleotide sequence complementary to a first portion of the SARS-CoV-2 N gene, and (ii) a second oligonucleotide primer comprising a nucleotide sequence complementary to an extension product formed from the first oligonucleotide primer of the secondary oligonucleotide primer set. The contacted sample is subjected to an amplification reaction under conditions suitable for producing transmembrane domain 2 gene and N gene amplification products, and the presence or absence of SARS-CoV-2 in the sample is detected. Isolated oligonucleotides, primer sets, and kits are also disclosed.