SMALL INTERFERING NUCLEIC ACID, AND COMPOSITION AND FISH PROBE GROUP THEREOF, PREPARATION METHOD THEREFOR AND USE THEREOF

摘要

Disclosed are a small interfering nucleic acid for inhibiting Coronavirus Disease 2019, and a composition and the use thereof. A piece of siRNA contains a sense chain and an anti-sense chain. The siRNA is 21-si-SP-2069, 21-si-SP-2289, 21-si-SP-3292, 21-si-P3-244, 21-si-P8-62, 21-si-P8-164, 21-si-P10B-158, 21-si-P14-19, 21-si-NP-722, 21-si-NP-1123, 21-si-NS-2139, 21-si-NS-2798, PP-1758, PP-17660, PP-20091, PP-20163, SP-26, SP-179, SP-2013, SP-2867, SP-3169, SP-3552, MG-83, NP-208, NP-241, 26280-EP, 26264-EP, 26356-EP, 26551-MP, 26580-MP, 26758-MP, 26787-MP, 27022-MP, 26607-MP, 29452-NP, 29406-NP, 23071-SP, 23250-SP, 25469-P3, 25631-P3, 25816-P3, 25652-P3, 26133-P3, 25936-P3 or 25897-P3, and some nucleotides of the sense chain or the anti-sense chain are 2'-O-methyl ribonucleotides and some phosphate groups thereof are thiophosphate groups. Also provided is an FISH probe group for detecting Coronavirus Disease 2019, SARS-CoV-2. Further provided are a new siRNA and a composition thereof, which can be used for effective prevention and/or treatment of Coronavirus Disease 2019. The FISH probe group has a high sensitivity and specificity, and can realize the location detection of SARS-CoV-2 in a sample to be detected to obtain the distribution and relative quantitative situation thereof.