Design and application of RNA binding protein using PPR motif

摘要

Problem to be solved: to provide a composition comprising RNA base selectively or RNA base sequence specific proteins.Protein of the inventionOne or more (preferably 2 to 14) PPR motifs comprising 30 to 38 amino acid long polypeptides expressed in formulaProtein (helix a) is12 amino acid lengthα A helix structure can be formed.Expression 2 andIn expressionA1-A12 represent amino acids independently;XIs not a part or 1 to 9 amino acid length;Helix B11 to 13 amino acid lengthsα Is a portion capable of forming a helical structure;L2 to 7 amino acid lengthsIs the expression represented by equationIn expression 3Amino acids"I" (- 1)"II" (- 2)And the C-terminal side numbering.HoweverLIII to lvii may not exist.The combinations of the three amino acids A1, A4, and LII or the combination of the two amino acids of A4 and II are based on the target RNA base or base sequence of the target RNA.No selection