Composition for simultaneous detection of swine enteric coronavirus and use thereof

摘要

The present invention relates to a composition for simultaneous detection of porcine enteritis coronavirus, comprising a primer and a probe set capable of simultaneously detecting porcine epidemic diarrhea virus and porcine infectious gastroenteritis virus as well as swine delta coronavirus, a novel coronavirus identified in pigs. Using the composition, the three types of viruses can be simultaneously detected and differentiated by a single PCR reaction through a multiplex RT-PCR method. When the primer and probe set of the present invention are used, it is possible to detect porcine enteritis coronavirus with high sensitivity and specificity, as well as reduce the time and cost for detection and secure the accuracy and ease of testing. It can be used as a useful technique for virus detection and surveillance.