mRNA display antibody library and methods

摘要

Compositions, methods and uses of high-diversity nucleic acid library that encodes a plurality of antibodies or antibody fragments are presented. The high-diversity nucleic acid library comprises or is derived from (1) a V_H-CDR1/2 sub-library, (2) a plurality of V_H-CDR3 sub-libraries, and (3) a V_L sub-library, each of which comprises a plurality of members. Preferably, each member of the sub-libraries comprises at least one random cassette that has a plurality of degenerate base positions. In an especially preferred embodiment, at least portions of at least two members of the V_H-CDR1/2 sub-library, the plurality of V_H-CDR3 sub-libraries, and the V_L sub-library are recombined to form an expression library member in an expression library, where each member of the expression library encodes a distinct antibody or antibody fragment.