Method and composition for producing chondrocyte lineage cell and / or cartilage like tissue

摘要

Methods and compositions for producing chondrocyte lineage cells and / or cartilage like tissues.Cartilage cells and / or cartilageOptionallyA method for fabricating a joint like non hypertrophic chondrocyte and / or cartilage like tissue and / or hypertrophic chondrocyte like cell and / or cartilage like tissueA. primitive lines of mesoderm cellsCultured with FGF agonists and BMP inhibitorsIdentifying the mesoderm population along the axisB.CD105 and / or PDGFR β Culture of the intermediate mesoderm group expressing the expression of the intermediate mesoderm population in serum unfertilized TGF β Cultured with an agonistSox9 +Steps comprising producing a collagen 2 + chondrocyte precursor population andC. to produce articular non hypertrophic chondrocytes and / or cartilage like tissues by culturing chondrocyte precursor populationsOr, comprising either hypertrophic chondrocyte like cells and / or steps of producing cartilage like tissueMethod.No selection