Cellular reprogramming to reverse aging and promote organ and tissue regeneration

摘要

OCT4 useful for inducing, for example, cell reprogramming, tissue repair, tissue regeneration, organ regeneration, reversal of senescence, or any combination thereof; KLF4; SOX2; Provided herein are engineered nucleic acids (e.g., expression vectors, such as viral vectors, such as lentiviral vectors, adenoviral vectors, AAV vectors, herpes virus vectors, and retroviral vectors) encoding any combination thereof. do. In addition, recombinant viruses (eg, lentiviruses, alphaviruses, vaccinia viruses, adenoviruses, herpes viruses, retroviruses, or AAVs), engineered cells, comprising engineered nucleic acids (eg, engineered nucleic acids) , a composition comprising: an engineered nucleic acid, a recombinant virus, an engineered cell, an engineered protein, OCT4; KLF4; SOX2; or a chemical agent capable of activating expression of any combination thereof, OCT4; KLF4; SOX2; or an engineered protein selected from the group consisting of any combination thereof, OCT4; KLF4; SOX2; or an antibody capable of activating expression of any combination thereof, and treatment (eg, ocular disease), prevention of disease (eg, ocular disease), modulation (eg, induction, or induction of cellular reprogramming), or cessation after induction), modulating tissue repair, modulating tissue regeneration, or any combination thereof are provided herein.