The present disclosure provides a bio-ink cartridge, a bio-ink cartridge assembly, a microsphere preparation device, a shell assembly device, a bio-block preparation instrument, a bio-ink preparation instrument and a bio-ink preparation system. The bio-ink cartridge comprises an ink cartridge shell with a cavity; a dividing wall arranged in the cavity and dividing the cavity into a first accommodating chamber and a second accommodating chamber independently; a first ink cartridge inlet and a first ink cartridge outlet which are arranged on the ink cartridge shell, communicated with the first accommodating chamber and separated from the second accommodating chamber; a second ink cartridge inlet and a second ink cartridge outlet which are arranged on the ink cartridge shell and communicated with the second accommodating chamber; and an inner flow channel located in the second accommodating chamber and communicated with the first accommodating chamber and the first ink cartridge outlet. The present disclosure is conducive to improve efficiencies of microsphere preparation, shell assembly and bio-block preparation. Further, the present disclosure is also conducive to improving activity of bio-block and intermediate products thereof.
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