INTRACELLULAR DELIVERY OF BIOMOLECULES FOR TOLERANCE INDUCTION

摘要

FIELD: biotechnology. ;SUBSTANCE: present invention relates to the field of biotechnology, specifically to cellular technologies, and can be used to induce tolerance or suppress the immune response to an antigen in an individual. The method of inducing tolerance or suppressing the immune response to an antigen involves passing a cell suspension containing an immune cell through a constriction, and the constriction deforms the cell, thereby causing a disturbance in the cell, so that the antigen and/or the tolerogenic factor penetrates the cell, thereby forming a tolerant and/or immunosuppressive immune cell. ;EFFECT: invention provides effective delivery of a payload-an antigen or a tolerogenic factor to immune cells, which can be used to induce immunity of the body to the specified agent.;200 cl, 12 dwg, 9 tbl, 5 ex