GENERATION OF INDUCED PLURIPOTENT STEM CELLS WITH POLYCISTRONIC SOX2, KLF4, AND OPTIONALLY C-MYC

摘要

Described herein a polycistronic expression cassettes and expression vectors that include a promoter operably linked to a nucleic acid segment that encodes a Sox2 and Klf4 polypeptide. The nucleic acid segment can also encode a c-Myc polypeptide. Expression of such polycistronic expression cassettes/vectors in host cells can reprogram the host cells to stem cells or other types of reprogrammed cells.