Binding proteins targeting GASP-1 granules and chimeric antigen receptor T cells, and uses thereof

摘要

A method for detecting cells having granules expressing G protein bound receptor-related sorting protein 1 (GASP-1) or a fragment thereof is provided. The detection method comprises the steps of (a) contacting a cell with an effective amount of a binding protein, the binding protein comprising an antigen-binding fragment that specifically binds to GASP-1; And (b) identifying cells having granules bound to the binding protein. GASP-1 granules can be present on the surface of cells or in the cytoplasm. Also provided are methods for generating T-cells comprising chimeric antigen receptors, anti-GASP-1 antibodies or bispecific binding proteins. Further provided are methods for treating GASP-1-mediated diseases or inactivating exosomes, microvesicles or oncosomes.