APPLICATION OF BRANCHED-CHAIN α-KETOACID DEHYDROGENASE COMPLEX IN PREPARATION OF MALONYL COENZYME A

摘要

An application of a branched-chain α-ketoacid dehydrogenase complex in preparation of malonyl coenzyme A. A method for preparing malonyl coenzyme A by using the branched-chain α-ketoacid dehydrogenase complex. The method comprises: introducing genes encoding the branched-chain α-ketoacid dehydrogenase complex into biological cells, and expressing the genes encoding the branched-chain α-ketoacid dehydrogenase complex to obtain recombinant cells; culturing the recombinant cells to obtain malonyl coenzyme A. The branched-chain α-ketoacid dehydrogenase complex is a complete-set protein consisting of M1) or M2): M1): bkdF, bkdG, bkdH, and lpdA1; and M2): bkdA, bkdB, bkdC, and lpdA1. Experimental results show that by using the branched-chain α-ketoacid dehydrogenase complex, not only malonyl coenzyme A can be prepared, but also a target product using malonyl coenzyme A as an intermediate product can further be prepared.