A biophysicochemical procedure for obtaining ribonucleic acid for experimental, biological, diagnostic and therapeutic applications. (Machine-translation by Google Translate, not legally binding)

摘要

A biophysical chemical procedure for obtaining ribonucleic acid for experimental, biological, diagnostic and therapeutic applications, characterized by comprising two fundamental parts, one physical and the other chemical, which correspond to the two phases of the process, that of rupture of the cytoplasmic membrane and that of isolation and purification of the biological product, the first consisting in resorting to a pressure comprised between 0.1 and 200 tons, or its equivalent in any other unit, increasing the pressure until obtaining a variable dependent on the type of cell from the that one wishes to obtain the rna; and maintaining the constant by increasing the pressure and decompressing sharply, repeating the process described three times. (Machine-translation by Google Translate, not legally binding)