Reagent for radio-immunoassay of testosterone - by reacting testerone-oxime with tyrosine ester

摘要

Testosterone-3-(O-carboxymethyl)-Oximetyrosine methyl ester is prepared by reacting testosterone-3-(O-carboxymethyl)-oxime and tyrosine methyl ester in the presence of N,N-dicyclohexylcarbodiimide in an apolar solvent at 0-25 degrees C. Then the solvent is evaporated and the crude residue is crystallised.