A process for producing a enzympraeparates and its use for carrying out biotech such reactions

摘要

1404933 Enzyme-protein complexes GISTBROCADES NV 8 Sept 1972 [24 Sept 1971] 44730/71 Heading C3H [Also in Division C2] A water-insoluble enzyme preparation in semi-solid or solid particulate form with the enzyme dispersed in the particles comprises bringing a mixture of a non-proteolytic enzyme and a gelling protein aqueous solution into particulate form, treating the resulting mixture in particulate form to effect gelation of the gelling protein, and treating the gelled protein in particulate form having said enzyme dispersed therein with a bi- or poly-functional protein reagent to cross-link molecules of the gelling protein and for molecules of the enzyme and the gelling protein to produce water-insoluble particles having enzyme dispersed therein. The gelling protein is usually gelatin or fresh egg white. The enzyme may be an invertase, lactase, maltase, amylase, urease, lipase, esterase, glucose isomerase, glucose oxidase, dehydrogenase or penicillinase and the bi- or polyfunctional reagent may be glutaric dialdehyde, arcolein, crotonaldehyde, a chloroformic ester, an acid chloride, epichlorohydrin, a derivative of dimethyladipic acid, a carbodiimide, phenol-2, 4-disulphonyl chloride, bromocyanide, a bromocyanide-activated compound of an acid halide or a mixture thereof. The aqueous solution may be brought into particulate form by mixing with an organic liquid, poorly immiscible or immiscible with water in such as way that the particulate form is obtained.