Antiviral interferon fixed to polysaccharide support - act on exterior of cells without effecting pathological processes inside cell

摘要

Interferon fixed to a support so that the interferon acts on the exterior of the cell without penetrating and effecting the pathological processing taking place within the cell is characterised in that the support is (a) a homopolysaccharide chosen from D-fructose polymers esp. laevosan (main link beta-2, 6-D-fructosyl-D-fructose), D-mannose polymers esp. mannan (main linkage: alpha-1,2, alpha-1,3 and alpha-1,6-D-mannosyl -D-mannose), D-galacturonate polymers esp. pectin (main linkage: alpha-1, 4-D-galacturonosyl-D-galacturonate), pentose polymers esp. xylan (main linkage : beta-1,4-D-xylosyl-D-xylose), D-galactose polymers esp. galactan (main linkage; beta-1,4-D-galactosyl-D-galactose) (b) a heteropolysaccharide containing modified sugars esp. powdered agarose (main linkage : beta-1,4-D-galactosyl-D-galactose), heteropolysaccharides containing substd. sugars esp. glycerol-pectate (main linkage : that of pectin) and derivatives containing more than one type of monosaccharide residue esp. heparin (main linkage alpha-1, 4-D-2-deoxy-2-sulphoamino-glucosyl-6-sulphate-D-Glucuronate) Antivval, treatment of certain forms of cancer and leukemia. When bonding in this way the interferon is not degraded by the cell and can be used in much smaller amounts. The supports are soluble or may be solubilised and the interferon may be administered by normal routes. The interferon is pref. fixed to a support activated by diazotisation esp. by the method of S.A. Barker et al (Immunology (72 23 689). Insoluble supports with fixed interferon may be solubilised by various treatments e.g. Sepharose (RTM) with fixed interferon may be hydrolysed by heating in an autoclave under 1 atmosphere and 110 degrees C for 39 mins at pH2.