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TRIPEPTIDE DERIVATIVES AND METHOD FOR THE QUANTITATIVE DETERMINATION OF PLASMINOGEN ACTIVATORS,INHIBITORS THEREOF AND TRYPSIN,TRYPSIN INHIBITORS AND TRYPSINOGEN IN HUMAN AND MAMMAL BODY FLUIDS AND TISSUE EXTRACTS THEREWITH
TRIPEPTIDE DERIVATIVES AND METHOD FOR THE QUANTITATIVE DETERMINATION OF PLASMINOGEN ACTIVATORS,INHIBITORS THEREOF AND TRYPSIN,TRYPSIN INHIBITORS AND TRYPSINOGEN IN HUMAN AND MAMMAL BODY FLUIDS AND TISSUE EXTRACTS THEREWITH
The present invention relates to a method for the quantitative determination of enzymes in human and mammal body fluids and tissue extracts, using a substrate which has the structure R1-X-Y-Z-NH-R2 wherein X represents a group having the formula IMAGE in which R3 is a straight or branched alkyl radical having 1 to 7 carbon atoms or a cyclohexyl or cyclohexylmethyl radical, Y represents a seryl group or a group having the formula -NH-(CH2)n-CO- in which n is an integer from 1 to 7, Z represents an arginyl or lysyl group, R1 represents hydrogen or an acyl or sulfonyl group and R2 represents an aromatic hydrocarbon radical which optionally may carry substituents. The method includes measuring by photometric, spectrophotometric or fluorescence-photometric methods the quantity of the split product NH2R2 formed by the hydrolytic action of the biologically active factors on the substrate.
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机译:本发明涉及使用具有结构R1-XYZ-NH-R2的底物,其中X代表具有式的基团的底物,定量测定人和哺乳动物的体液和组织提取物中的酶的方法。 R 3为具有1至7个碳原子的直链或支链烷基或环己基或环己基甲基基团,Y表示丝氨酸基或具有式-NH-(CH 2)n -CO-的基团,其中n为1的整数。在图7至7中,Z代表精氨酰基或赖氨酰基,R1代表氢或酰基或磺酰基,R2代表可任选带有取代基的芳族烃基。该方法包括通过光度法,分光光度法或荧光光度法测量由生物活性因子在底物上的水解作用形成的分裂产物NH 2 R 2的量。
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