Desacetylthymosin alpha 1, its process of preparation and its use

摘要

The present invention concerns desacetylthymosin alpha 1, an immune restorative polypeptide hormone. This polypeptide is synthesized in E. coli using recombinant DNA cloning techniques. Based on the known amino acid sequence, a gene coding for thymosin alpha 1 was designed and enzymatically assembled from chemically synthesized oligodeoxyribonucleotide fragments. The gene was ligated into plasmid pBR322, placed under lac operon control, and thymosin alpha 1 expressed as part of a β-galactosidase chimeric protein. Cyanogen bromide cleavage of this protein gave a mixture of polypeptides among which thymosin alpha 1 was detected by radioimmunoassay. The E. coli product is identical to thymosin alpha 1 isolated from calf thymus in amino acid sequence and exhibits similar activity in a radioimmunoassay.;The compound is hence a candidate for administration in effective immunopotentiating dosage in the treatment of immuno-depressed and immuno-suppressed conditions.