PREPARATION OF LICHEN COMPONENT BY TISSUE CULTURE OF LICHEN

摘要

PURPOSE:To prepare a desired lichen component, stably, rapidly, in high yield, by the tissue culture of the undifferentiated symbiont derived from the tissue of lichen. CONSTITUTION:The tissue of lichen is cultured in a proper medium at a constant temperature between 0 and 40 deg.C usually in a light place, and the undifferentiated symbiont formed on the surface of the lichen is aseptically transplanted to a new medium having a proper composition and cultured at a constant temperature between 0 and 40 deg.C, preferably 20 and 35 deg.C, preferably in a liquid medium under shaking or agitation and aeration. The objective lichen component can be separated from the cultured and proliferated undifferentiated lichen symbiont.