首页> 外国专利> B-type hepatitis biruseu Cao Huan plasmid insert a gene, the method has been switched to Cao Huan plasmid and yeast hepatitis b surface antigen biruseu

B-type hepatitis biruseu Cao Huan plasmid insert a gene, the method has been switched to Cao Huan plasmid and yeast hepatitis b surface antigen biruseu

机译:B型丙型肝炎病毒曹焕质粒插入一个基因,该方法已转换为曹焕质粒和酵母乙型肝炎表面抗原biruseu

摘要

A method for preparing hepatitis B virus surface antigen is presented. Thus, saccharomyces cerevisiae pAH 203, which was cultured in Burk Holder minimal medium contg. 20 g/ml histidine at 30C for 24hr, was suspended in 50mM phosphate buffer (pH=7.2) contg. 1.2M sorbitol, 14mM 2-mercaptoethanol, and 100 g/ml zymolyase-60,000 and shaked at 30C for 30min to give spheroplast followed by suspending it in a 50mM phosphate buffer contg. 0.1% Triton X-100 to give yeast lysate.
机译:提供了一种制备乙型肝炎病毒表面抗原的方法。因此,在Burk Holder基本培养基中培养的酿酒酵母pAH 203。在30℃将20μg/ ml组氨酸悬浮24小时,将其悬浮在50mM磷酸盐缓冲液(pH = 7.2)中。将1.2M山梨糖醇,14mM 2-巯基乙醇和100μg/ ml 60,000 zymolyase酶在30℃下摇动30分钟,得到原生质球,然后将其悬浮在50mM磷酸缓冲液中。 0.1%Triton X-100产生酵母裂解物。

著录项

  • 公开/公告号KR840005745A

    专利类型

  • 公开/公告日1984-11-16

    原文格式PDF

  • 申请/专利权人 로꾸단다 도오끼찌;

    申请/专利号KR19830003794

  • 发明设计人 미야노하라 아쓰시 (외 5);

    申请日1983-08-13

  • 分类号C12P21/02;

  • 国家 KR

  • 入库时间 2022-08-22 08:01:16

相似文献

  • 专利
  • 外文文献
  • 中文文献
获取专利

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号